Cargando…

Differential gene expression in the calvarial and cortical bone of juvenile female mice

INTRODUCTION: Both the calvarial and the cortical bones develop through intramembranous ossification, yet they have very different structures and functions. The calvaria enables the rapid while protected growth of the brain, whereas the cortical bone takes part in locomotion. Both types of bones und...

Descripción completa

Detalles Bibliográficos
Autores principales: Janssen, Jerome Nicolas, Kalev-Altman, Rotem, Shalit, Tali, Sela-Donenfeld, Dalit, Monsonego-Ornan, Efrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291685/
https://www.ncbi.nlm.nih.gov/pubmed/37378024
http://dx.doi.org/10.3389/fendo.2023.1127536
_version_ 1785062738258558976
author Janssen, Jerome Nicolas
Kalev-Altman, Rotem
Shalit, Tali
Sela-Donenfeld, Dalit
Monsonego-Ornan, Efrat
author_facet Janssen, Jerome Nicolas
Kalev-Altman, Rotem
Shalit, Tali
Sela-Donenfeld, Dalit
Monsonego-Ornan, Efrat
author_sort Janssen, Jerome Nicolas
collection PubMed
description INTRODUCTION: Both the calvarial and the cortical bones develop through intramembranous ossification, yet they have very different structures and functions. The calvaria enables the rapid while protected growth of the brain, whereas the cortical bone takes part in locomotion. Both types of bones undergo extensive modeling during embryonic and post-natal growth, while bone remodeling is the most dominant process in adults. Their shared formation mechanism and their highly distinct functions raise the fundamental question of how similar or diverse the molecular pathways that act in each bone type are. METHODS: To answer this question, we aimed to compare the transcriptomes of calvaria and cortices from 21-day old mice by bulk RNA-Seq analysis. RESULTS: The results revealed clear differences in expression levels of genes related to bone pathologies, craniosynostosis, mechanical loading and bone-relevant signaling pathways like WNT and IHH, emphasizing the functional differences between these bones. We further discussed the less expected candidate genes and gene sets in the context of bone. Finally, we compared differences between juvenile and mature bone, highlighting commonalities and dissimilarities of gene expression between calvaria and cortices during post-natal bone growth and adult bone remodeling. DISCUSSION: Altogether, this study revealed significant differences between the transcriptome of calvaria and cortical bones in juvenile female mice, highlighting the most important pathway mediators for the development and function of two different bone types that originate both through intramembranous ossification.
format Online
Article
Text
id pubmed-10291685
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102916852023-06-27 Differential gene expression in the calvarial and cortical bone of juvenile female mice Janssen, Jerome Nicolas Kalev-Altman, Rotem Shalit, Tali Sela-Donenfeld, Dalit Monsonego-Ornan, Efrat Front Endocrinol (Lausanne) Endocrinology INTRODUCTION: Both the calvarial and the cortical bones develop through intramembranous ossification, yet they have very different structures and functions. The calvaria enables the rapid while protected growth of the brain, whereas the cortical bone takes part in locomotion. Both types of bones undergo extensive modeling during embryonic and post-natal growth, while bone remodeling is the most dominant process in adults. Their shared formation mechanism and their highly distinct functions raise the fundamental question of how similar or diverse the molecular pathways that act in each bone type are. METHODS: To answer this question, we aimed to compare the transcriptomes of calvaria and cortices from 21-day old mice by bulk RNA-Seq analysis. RESULTS: The results revealed clear differences in expression levels of genes related to bone pathologies, craniosynostosis, mechanical loading and bone-relevant signaling pathways like WNT and IHH, emphasizing the functional differences between these bones. We further discussed the less expected candidate genes and gene sets in the context of bone. Finally, we compared differences between juvenile and mature bone, highlighting commonalities and dissimilarities of gene expression between calvaria and cortices during post-natal bone growth and adult bone remodeling. DISCUSSION: Altogether, this study revealed significant differences between the transcriptome of calvaria and cortical bones in juvenile female mice, highlighting the most important pathway mediators for the development and function of two different bone types that originate both through intramembranous ossification. Frontiers Media S.A. 2023-06-12 /pmc/articles/PMC10291685/ /pubmed/37378024 http://dx.doi.org/10.3389/fendo.2023.1127536 Text en Copyright © 2023 Janssen, Kalev-Altman, Shalit, Sela-Donenfeld and Monsonego-Ornan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Janssen, Jerome Nicolas
Kalev-Altman, Rotem
Shalit, Tali
Sela-Donenfeld, Dalit
Monsonego-Ornan, Efrat
Differential gene expression in the calvarial and cortical bone of juvenile female mice
title Differential gene expression in the calvarial and cortical bone of juvenile female mice
title_full Differential gene expression in the calvarial and cortical bone of juvenile female mice
title_fullStr Differential gene expression in the calvarial and cortical bone of juvenile female mice
title_full_unstemmed Differential gene expression in the calvarial and cortical bone of juvenile female mice
title_short Differential gene expression in the calvarial and cortical bone of juvenile female mice
title_sort differential gene expression in the calvarial and cortical bone of juvenile female mice
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291685/
https://www.ncbi.nlm.nih.gov/pubmed/37378024
http://dx.doi.org/10.3389/fendo.2023.1127536
work_keys_str_mv AT janssenjeromenicolas differentialgeneexpressioninthecalvarialandcorticalboneofjuvenilefemalemice
AT kalevaltmanrotem differentialgeneexpressioninthecalvarialandcorticalboneofjuvenilefemalemice
AT shalittali differentialgeneexpressioninthecalvarialandcorticalboneofjuvenilefemalemice
AT seladonenfelddalit differentialgeneexpressioninthecalvarialandcorticalboneofjuvenilefemalemice
AT monsonegoornanefrat differentialgeneexpressioninthecalvarialandcorticalboneofjuvenilefemalemice