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Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway
Clear cell renal cell carcinoma (ccRCC) is the most common solid renal tumor. NSUN5, a gene encoding cytosine-5 RNA methyltransferase, has rarely been reported associated with cancer. A bioinformatics analysis revealed that NSUN5 was overexpressed in ccRCC. Gene Ontology and gene set variation analy...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10292888/ https://www.ncbi.nlm.nih.gov/pubmed/37263638 http://dx.doi.org/10.18632/aging.204761 |
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author | Li, Lei Li, Mingyang Zheng, Jianyi Li, Zeyu Chen, Xiaonan |
author_facet | Li, Lei Li, Mingyang Zheng, Jianyi Li, Zeyu Chen, Xiaonan |
author_sort | Li, Lei |
collection | PubMed |
description | Clear cell renal cell carcinoma (ccRCC) is the most common solid renal tumor. NSUN5, a gene encoding cytosine-5 RNA methyltransferase, has rarely been reported associated with cancer. A bioinformatics analysis revealed that NSUN5 was overexpressed in ccRCC. Gene Ontology and gene set variation analyses showed that NSUN5 was associated with tumor immunity in ccRCC. The effect of immunosuppressive treatment was superior in the low-risk group compared to the high-risk group, and higher stromal score in the high-risk group relative to the low-risk group. A drug sensitivity analysis revealed that the high-risk group was more sensitive to 5-fluorouracil, mitomycin C, methotrexate, and 17-AAG, whereas the low-risk group was more sensitive to crizotinib, sorafenib, foretinib, and ivozanib. NSUN5 knockout decreased ccRCC cell proliferation. The migration speed and number of invasive cells further decreased. The percentage of apoptotic cells increased. In NSUN5-knockout cells, the levels of BAX, caspase-8, caspase-9, and p53 increased significantly, whereas those of Bcl2, CCND1, CCND3, and MMP9 decreased significantly. NSUN5 is highly expressed in ccRCC and inhibits cancer cell invasion, proliferation, and migration while promoting apoptosis by activating the p53 signaling pathway. This study provides insights into the mechanisms of action of NSUN5 in urological tumors and may contribute to improving ccRCC treatment options. |
format | Online Article Text |
id | pubmed-10292888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-102928882023-06-27 Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway Li, Lei Li, Mingyang Zheng, Jianyi Li, Zeyu Chen, Xiaonan Aging (Albany NY) Research Paper Clear cell renal cell carcinoma (ccRCC) is the most common solid renal tumor. NSUN5, a gene encoding cytosine-5 RNA methyltransferase, has rarely been reported associated with cancer. A bioinformatics analysis revealed that NSUN5 was overexpressed in ccRCC. Gene Ontology and gene set variation analyses showed that NSUN5 was associated with tumor immunity in ccRCC. The effect of immunosuppressive treatment was superior in the low-risk group compared to the high-risk group, and higher stromal score in the high-risk group relative to the low-risk group. A drug sensitivity analysis revealed that the high-risk group was more sensitive to 5-fluorouracil, mitomycin C, methotrexate, and 17-AAG, whereas the low-risk group was more sensitive to crizotinib, sorafenib, foretinib, and ivozanib. NSUN5 knockout decreased ccRCC cell proliferation. The migration speed and number of invasive cells further decreased. The percentage of apoptotic cells increased. In NSUN5-knockout cells, the levels of BAX, caspase-8, caspase-9, and p53 increased significantly, whereas those of Bcl2, CCND1, CCND3, and MMP9 decreased significantly. NSUN5 is highly expressed in ccRCC and inhibits cancer cell invasion, proliferation, and migration while promoting apoptosis by activating the p53 signaling pathway. This study provides insights into the mechanisms of action of NSUN5 in urological tumors and may contribute to improving ccRCC treatment options. Impact Journals 2023-06-01 /pmc/articles/PMC10292888/ /pubmed/37263638 http://dx.doi.org/10.18632/aging.204761 Text en Copyright: © 2023 Li et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Li, Lei Li, Mingyang Zheng, Jianyi Li, Zeyu Chen, Xiaonan Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway |
title | Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway |
title_full | Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway |
title_fullStr | Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway |
title_full_unstemmed | Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway |
title_short | Knocking down NSUN5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway |
title_sort | knocking down nsun5 inhibits the development of clear cell renal cell carcinoma by inhibiting the p53 pathway |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10292888/ https://www.ncbi.nlm.nih.gov/pubmed/37263638 http://dx.doi.org/10.18632/aging.204761 |
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