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Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm

Basal stem rot due to a fungal pathogen, Ganoderma boninense, is one of the most devastating diseases in oil palm throughout the major palm oil producer countries. This study investigated the potential of polypore fungi as biological control agents against pathogenic G. boninense in oil palm. In vit...

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Autores principales: Darlis, Darwana, Jalloh, Mohamadu Boyie, Chin, Clament Fui Seung, Basri, Noor Khairani Mohamad, Besar, Normah Awang, Ahmad, Khairulmazmi, Rakib, Mohd. Rashid Mohd.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293268/
https://www.ncbi.nlm.nih.gov/pubmed/37365214
http://dx.doi.org/10.1038/s41598-023-37507-0
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author Darlis, Darwana
Jalloh, Mohamadu Boyie
Chin, Clament Fui Seung
Basri, Noor Khairani Mohamad
Besar, Normah Awang
Ahmad, Khairulmazmi
Rakib, Mohd. Rashid Mohd.
author_facet Darlis, Darwana
Jalloh, Mohamadu Boyie
Chin, Clament Fui Seung
Basri, Noor Khairani Mohamad
Besar, Normah Awang
Ahmad, Khairulmazmi
Rakib, Mohd. Rashid Mohd.
author_sort Darlis, Darwana
collection PubMed
description Basal stem rot due to a fungal pathogen, Ganoderma boninense, is one of the most devastating diseases in oil palm throughout the major palm oil producer countries. This study investigated the potential of polypore fungi as biological control agents against pathogenic G. boninense in oil palm. In vitro antagonistic screening of selected non-pathogenic polypore fungi was performed. Based on in planta fungi inoculation on oil palm seedlings, eight of the 21 fungi isolates tested (GL01, GL01, RDC06, RDC24, SRP11, SRP12, SRP17, and SRP18) were non-pathogenic. In vitro antagonistic assays against G. boninense revealed that the percentage inhibition of radial growth (PIRG) in dual culture assay for SRP11 (69.7%), SRP17 (67.3%), and SRP18 (72.7%) was relatively high. Percentage inhibition of diameter growth (PIDG) in volatile organic compounds (VOCs) in dual plate assay of SRP11, SRP17, and SRP18 isolates were 43.2%, 51.6%, and 52.1%, respectively. Molecular identification using the internal transcribed spacer gene sequences of SRP11, SRP17, and SRP18 isolates revealed that they were Fomes sp., Trametes elegans, and Trametes lactinea, respectively.
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spelling pubmed-102932682023-06-28 Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm Darlis, Darwana Jalloh, Mohamadu Boyie Chin, Clament Fui Seung Basri, Noor Khairani Mohamad Besar, Normah Awang Ahmad, Khairulmazmi Rakib, Mohd. Rashid Mohd. Sci Rep Article Basal stem rot due to a fungal pathogen, Ganoderma boninense, is one of the most devastating diseases in oil palm throughout the major palm oil producer countries. This study investigated the potential of polypore fungi as biological control agents against pathogenic G. boninense in oil palm. In vitro antagonistic screening of selected non-pathogenic polypore fungi was performed. Based on in planta fungi inoculation on oil palm seedlings, eight of the 21 fungi isolates tested (GL01, GL01, RDC06, RDC24, SRP11, SRP12, SRP17, and SRP18) were non-pathogenic. In vitro antagonistic assays against G. boninense revealed that the percentage inhibition of radial growth (PIRG) in dual culture assay for SRP11 (69.7%), SRP17 (67.3%), and SRP18 (72.7%) was relatively high. Percentage inhibition of diameter growth (PIDG) in volatile organic compounds (VOCs) in dual plate assay of SRP11, SRP17, and SRP18 isolates were 43.2%, 51.6%, and 52.1%, respectively. Molecular identification using the internal transcribed spacer gene sequences of SRP11, SRP17, and SRP18 isolates revealed that they were Fomes sp., Trametes elegans, and Trametes lactinea, respectively. Nature Publishing Group UK 2023-06-26 /pmc/articles/PMC10293268/ /pubmed/37365214 http://dx.doi.org/10.1038/s41598-023-37507-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Darlis, Darwana
Jalloh, Mohamadu Boyie
Chin, Clament Fui Seung
Basri, Noor Khairani Mohamad
Besar, Normah Awang
Ahmad, Khairulmazmi
Rakib, Mohd. Rashid Mohd.
Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm
title Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm
title_full Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm
title_fullStr Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm
title_full_unstemmed Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm
title_short Exploring the potential of Bornean polypore fungi as biological control agents against pathogenic Ganoderma boninense causing basal stem rot in oil palm
title_sort exploring the potential of bornean polypore fungi as biological control agents against pathogenic ganoderma boninense causing basal stem rot in oil palm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293268/
https://www.ncbi.nlm.nih.gov/pubmed/37365214
http://dx.doi.org/10.1038/s41598-023-37507-0
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