Cargando…
High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma
BACKGROUND: Bromodomain-containing protein 4 (BRD4) is a reader of histone acetylation and is associated with a variety of diseases. AIM: To investigate the expression level of BRD4 in esophageal squamous cell carcinoma (ESCC), its prognostic value and its relationship with immune infiltration. METH...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293338/ https://www.ncbi.nlm.nih.gov/pubmed/36933111 http://dx.doi.org/10.1007/s10620-023-07907-3 |
_version_ | 1785062980132536320 |
---|---|
author | Li, Li Gao, Lin Zhou, Hong Shi, Chao Zhang, Xiaojuan Zhang, Dongmei Liu, Hongbin |
author_facet | Li, Li Gao, Lin Zhou, Hong Shi, Chao Zhang, Xiaojuan Zhang, Dongmei Liu, Hongbin |
author_sort | Li, Li |
collection | PubMed |
description | BACKGROUND: Bromodomain-containing protein 4 (BRD4) is a reader of histone acetylation and is associated with a variety of diseases. AIM: To investigate the expression level of BRD4 in esophageal squamous cell carcinoma (ESCC), its prognostic value and its relationship with immune infiltration. METHODS: The study included 94 ESCC patients from The Cancer Genome Atlas (TCGA) database and 179 ESCC patients from Affiliated Hospital 2 of Nantong University. The expression levels of proteins in tissue microarray were detected by immunohistochemistry. The prognostic factors were analyzed by Kaplan–Meier curve and univariate and multivariate cox regression. The ESTIMATE website was used to calculate the stromal, immune and ESTIMATE score. CIBERSORT was used to calculate the abundance of immune infiltrates. Spearman and Phi coefficient were used for correlation analysis. The TIDE algorithm was used to predict treatment response to immune checkpoint blockade. RESULTS: BRD4 is up-regulated in ESCC, and high BRD4 expression level is associated with poor prognosis and adverse clinicopathological features. In addition, the monocyte count, systemic inflammatory-immunologic index, platelet-lymphocyte ratio, and monocyte-lymphocyte ratio in the BRD4 high expression level group were higher than in the low expression level group. Finally, we found that BRD4 expression level correlated with immune infiltration and that it was inversely correlated with infiltration of CD8 + T cells. Higher TIDE scores in the BRD4 high expression group than in the low expression group. CONCLUSION: BRD4 is associated with poor prognosis and immune infiltration in ESCC, and may be a potential biomarker for prognosis and immunotherapy application. |
format | Online Article Text |
id | pubmed-10293338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-102933382023-06-28 High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma Li, Li Gao, Lin Zhou, Hong Shi, Chao Zhang, Xiaojuan Zhang, Dongmei Liu, Hongbin Dig Dis Sci Original Article BACKGROUND: Bromodomain-containing protein 4 (BRD4) is a reader of histone acetylation and is associated with a variety of diseases. AIM: To investigate the expression level of BRD4 in esophageal squamous cell carcinoma (ESCC), its prognostic value and its relationship with immune infiltration. METHODS: The study included 94 ESCC patients from The Cancer Genome Atlas (TCGA) database and 179 ESCC patients from Affiliated Hospital 2 of Nantong University. The expression levels of proteins in tissue microarray were detected by immunohistochemistry. The prognostic factors were analyzed by Kaplan–Meier curve and univariate and multivariate cox regression. The ESTIMATE website was used to calculate the stromal, immune and ESTIMATE score. CIBERSORT was used to calculate the abundance of immune infiltrates. Spearman and Phi coefficient were used for correlation analysis. The TIDE algorithm was used to predict treatment response to immune checkpoint blockade. RESULTS: BRD4 is up-regulated in ESCC, and high BRD4 expression level is associated with poor prognosis and adverse clinicopathological features. In addition, the monocyte count, systemic inflammatory-immunologic index, platelet-lymphocyte ratio, and monocyte-lymphocyte ratio in the BRD4 high expression level group were higher than in the low expression level group. Finally, we found that BRD4 expression level correlated with immune infiltration and that it was inversely correlated with infiltration of CD8 + T cells. Higher TIDE scores in the BRD4 high expression group than in the low expression group. CONCLUSION: BRD4 is associated with poor prognosis and immune infiltration in ESCC, and may be a potential biomarker for prognosis and immunotherapy application. Springer US 2023-03-18 2023 /pmc/articles/PMC10293338/ /pubmed/36933111 http://dx.doi.org/10.1007/s10620-023-07907-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/Open AccessThis article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Original Article Li, Li Gao, Lin Zhou, Hong Shi, Chao Zhang, Xiaojuan Zhang, Dongmei Liu, Hongbin High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma |
title | High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma |
title_full | High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma |
title_fullStr | High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma |
title_full_unstemmed | High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma |
title_short | High Expression Level of BRD4 Is Associated with a Poor Prognosis and Immune Infiltration in Esophageal Squamous Cell Carcinoma |
title_sort | high expression level of brd4 is associated with a poor prognosis and immune infiltration in esophageal squamous cell carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293338/ https://www.ncbi.nlm.nih.gov/pubmed/36933111 http://dx.doi.org/10.1007/s10620-023-07907-3 |
work_keys_str_mv | AT lili highexpressionlevelofbrd4isassociatedwithapoorprognosisandimmuneinfiltrationinesophagealsquamouscellcarcinoma AT gaolin highexpressionlevelofbrd4isassociatedwithapoorprognosisandimmuneinfiltrationinesophagealsquamouscellcarcinoma AT zhouhong highexpressionlevelofbrd4isassociatedwithapoorprognosisandimmuneinfiltrationinesophagealsquamouscellcarcinoma AT shichao highexpressionlevelofbrd4isassociatedwithapoorprognosisandimmuneinfiltrationinesophagealsquamouscellcarcinoma AT zhangxiaojuan highexpressionlevelofbrd4isassociatedwithapoorprognosisandimmuneinfiltrationinesophagealsquamouscellcarcinoma AT zhangdongmei highexpressionlevelofbrd4isassociatedwithapoorprognosisandimmuneinfiltrationinesophagealsquamouscellcarcinoma AT liuhongbin highexpressionlevelofbrd4isassociatedwithapoorprognosisandimmuneinfiltrationinesophagealsquamouscellcarcinoma |