Cargando…

Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report

BACKGROUND: The forming, blending, and characterization of materials at a size of one billionth of a meter or less is referred to as nanotechnology. The objective of the current study was to synthesize ecologically friendly gold nanoparticles (AuNPs) from Gymnosporia montana L. (G. montana) leaf ext...

Descripción completa

Detalles Bibliográficos
Autores principales: Patel, Krishnakumari N., Trivedi, Pooja G., Thakar, Milan S., Prajapati, Kush V., Prajapati, Dhruv K., Sindhav, Gaurang M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293534/
https://www.ncbi.nlm.nih.gov/pubmed/37358697
http://dx.doi.org/10.1186/s43141-023-00525-6
_version_ 1785063014341279744
author Patel, Krishnakumari N.
Trivedi, Pooja G.
Thakar, Milan S.
Prajapati, Kush V.
Prajapati, Dhruv K.
Sindhav, Gaurang M.
author_facet Patel, Krishnakumari N.
Trivedi, Pooja G.
Thakar, Milan S.
Prajapati, Kush V.
Prajapati, Dhruv K.
Sindhav, Gaurang M.
author_sort Patel, Krishnakumari N.
collection PubMed
description BACKGROUND: The forming, blending, and characterization of materials at a size of one billionth of a meter or less is referred to as nanotechnology. The objective of the current study was to synthesize ecologically friendly gold nanoparticles (AuNPs) from Gymnosporia montana L. (G. montana) leaf extract, characterize them, assess their interaction with different types of deoxyribonucleic acid (DNA), and investigate their antioxidant and toxic capabilities. RESULTS: The biosynthesized AuNPs presence was validated by a color change from yellow to reddish pink as well as using UV–visible spectrophotometer. Fourier transform infrared (FTIR) spectroscopy analysis showed the presence of phytoconstituents like, alcohols, phenols, and nitro compounds responsible for the reduction of AuNPs. Zeta sizer and zeta potential of 559.6 d. nm and − 4.5 mV, respectively, demonstrated potential stability. With an average size between 10 and 50 nm, X-ray diffraction (XRD), and high-resolution transmission electron microscope (HR-TEM), revealed the crystalline formation of AuNPs. Surface topology with 3D characterization, irregular spherical shape, and size with 6.48 nm of AuNPs was determined with the help of an atomic force microscope (AFM). AuNPs with some irregular and spherical shapes, and sizes between 2 and 20 nm, were revealed by field emission scanning electron microscope (FESEM) investigation. Shifts in the spectrum were visible when the bioavailability of AuNPs with calf-thymus DNA (CT-DNA) and Herring sperm DNA (HS-DNA) was tested. Additionally, the DNA nicking assay’s interaction with pBR322 DNA confirmed its physiochemical and antioxidant properties. The same was also found by using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, which showed a 70–80% inhibition rate. Finally, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay revealed that viability decreased with increasing dosage, going from 77.74 to 46.99% on MCF-7 cell line. CONCLUSION: Synthesizing AuNPs through biogenic processes and adopting G. montana for the first time revealed potential DNA interaction, antioxidant, and cytotoxicity capabilities. Thus, opening new possibilities in the turf of therapeutics as well as in other areas. GRAPHICAL ABSTRACT: [Image: see text]
format Online
Article
Text
id pubmed-10293534
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-102935342023-06-28 Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report Patel, Krishnakumari N. Trivedi, Pooja G. Thakar, Milan S. Prajapati, Kush V. Prajapati, Dhruv K. Sindhav, Gaurang M. J Genet Eng Biotechnol Research BACKGROUND: The forming, blending, and characterization of materials at a size of one billionth of a meter or less is referred to as nanotechnology. The objective of the current study was to synthesize ecologically friendly gold nanoparticles (AuNPs) from Gymnosporia montana L. (G. montana) leaf extract, characterize them, assess their interaction with different types of deoxyribonucleic acid (DNA), and investigate their antioxidant and toxic capabilities. RESULTS: The biosynthesized AuNPs presence was validated by a color change from yellow to reddish pink as well as using UV–visible spectrophotometer. Fourier transform infrared (FTIR) spectroscopy analysis showed the presence of phytoconstituents like, alcohols, phenols, and nitro compounds responsible for the reduction of AuNPs. Zeta sizer and zeta potential of 559.6 d. nm and − 4.5 mV, respectively, demonstrated potential stability. With an average size between 10 and 50 nm, X-ray diffraction (XRD), and high-resolution transmission electron microscope (HR-TEM), revealed the crystalline formation of AuNPs. Surface topology with 3D characterization, irregular spherical shape, and size with 6.48 nm of AuNPs was determined with the help of an atomic force microscope (AFM). AuNPs with some irregular and spherical shapes, and sizes between 2 and 20 nm, were revealed by field emission scanning electron microscope (FESEM) investigation. Shifts in the spectrum were visible when the bioavailability of AuNPs with calf-thymus DNA (CT-DNA) and Herring sperm DNA (HS-DNA) was tested. Additionally, the DNA nicking assay’s interaction with pBR322 DNA confirmed its physiochemical and antioxidant properties. The same was also found by using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, which showed a 70–80% inhibition rate. Finally, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay revealed that viability decreased with increasing dosage, going from 77.74 to 46.99% on MCF-7 cell line. CONCLUSION: Synthesizing AuNPs through biogenic processes and adopting G. montana for the first time revealed potential DNA interaction, antioxidant, and cytotoxicity capabilities. Thus, opening new possibilities in the turf of therapeutics as well as in other areas. GRAPHICAL ABSTRACT: [Image: see text] Springer Berlin Heidelberg 2023-06-26 /pmc/articles/PMC10293534/ /pubmed/37358697 http://dx.doi.org/10.1186/s43141-023-00525-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Patel, Krishnakumari N.
Trivedi, Pooja G.
Thakar, Milan S.
Prajapati, Kush V.
Prajapati, Dhruv K.
Sindhav, Gaurang M.
Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report
title Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report
title_full Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report
title_fullStr Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report
title_full_unstemmed Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report
title_short Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report
title_sort gold nanoparticles synthesis using gymnosporia montana l. and its biological profile: a pioneer report
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293534/
https://www.ncbi.nlm.nih.gov/pubmed/37358697
http://dx.doi.org/10.1186/s43141-023-00525-6
work_keys_str_mv AT patelkrishnakumarin goldnanoparticlessynthesisusinggymnosporiamontanalanditsbiologicalprofileapioneerreport
AT trivedipoojag goldnanoparticlessynthesisusinggymnosporiamontanalanditsbiologicalprofileapioneerreport
AT thakarmilans goldnanoparticlessynthesisusinggymnosporiamontanalanditsbiologicalprofileapioneerreport
AT prajapatikushv goldnanoparticlessynthesisusinggymnosporiamontanalanditsbiologicalprofileapioneerreport
AT prajapatidhruvk goldnanoparticlessynthesisusinggymnosporiamontanalanditsbiologicalprofileapioneerreport
AT sindhavgaurangm goldnanoparticlessynthesisusinggymnosporiamontanalanditsbiologicalprofileapioneerreport