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Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein
Urine-derived stem cells (USCs) are a promising source for regenerative medicine because of their advantages such as easy and non-invasive collection from the human body, stable expansion, and the potential to differentiate into multiple lineages, including osteoblasts. In this study, we propose a s...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293758/ https://www.ncbi.nlm.nih.gov/pubmed/37383520 http://dx.doi.org/10.3389/fbioe.2023.1215087 |
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author | Park, Jinhee Jeong, Kiho Kim, Manho Kim, Wijin Park, Ju Hyun |
author_facet | Park, Jinhee Jeong, Kiho Kim, Manho Kim, Wijin Park, Ju Hyun |
author_sort | Park, Jinhee |
collection | PubMed |
description | Urine-derived stem cells (USCs) are a promising source for regenerative medicine because of their advantages such as easy and non-invasive collection from the human body, stable expansion, and the potential to differentiate into multiple lineages, including osteoblasts. In this study, we propose a strategy to enhance the osteogenic potential of human USCs using Lin28A, a transcription factor that inhibits let-7 miRNA processing. To address concerns regarding the safety of foreign gene integration and potential risk of tumorigenicity, we intracellularly delivered Lin28A as a recombinant protein fused with a cell-penetrating and protein-stabilizing protein, 30Kc19α. 30Kc19α–Lin28A fusion protein exhibited improved thermal stability and was delivered into USCs without significant cytotoxicity. 30Kc19α–Lin28A treatment elevated calcium deposition and upregulated several osteoblast-specific gene expressions in USCs derived from multiple donors. Our results indicate that intracellularly delivered 30Kc19α–Lin28A enhances the osteoblastic differentiation of human USCs by affecting the transcriptional regulatory network involved in metabolic reprogramming and stem cell potency. Therefore, 30Kc19α–Lin28A may provide a technical advancement toward developing clinically feasible strategies for bone regeneration. |
format | Online Article Text |
id | pubmed-10293758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102937582023-06-28 Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein Park, Jinhee Jeong, Kiho Kim, Manho Kim, Wijin Park, Ju Hyun Front Bioeng Biotechnol Bioengineering and Biotechnology Urine-derived stem cells (USCs) are a promising source for regenerative medicine because of their advantages such as easy and non-invasive collection from the human body, stable expansion, and the potential to differentiate into multiple lineages, including osteoblasts. In this study, we propose a strategy to enhance the osteogenic potential of human USCs using Lin28A, a transcription factor that inhibits let-7 miRNA processing. To address concerns regarding the safety of foreign gene integration and potential risk of tumorigenicity, we intracellularly delivered Lin28A as a recombinant protein fused with a cell-penetrating and protein-stabilizing protein, 30Kc19α. 30Kc19α–Lin28A fusion protein exhibited improved thermal stability and was delivered into USCs without significant cytotoxicity. 30Kc19α–Lin28A treatment elevated calcium deposition and upregulated several osteoblast-specific gene expressions in USCs derived from multiple donors. Our results indicate that intracellularly delivered 30Kc19α–Lin28A enhances the osteoblastic differentiation of human USCs by affecting the transcriptional regulatory network involved in metabolic reprogramming and stem cell potency. Therefore, 30Kc19α–Lin28A may provide a technical advancement toward developing clinically feasible strategies for bone regeneration. Frontiers Media S.A. 2023-06-13 /pmc/articles/PMC10293758/ /pubmed/37383520 http://dx.doi.org/10.3389/fbioe.2023.1215087 Text en Copyright © 2023 Park, Jeong, Kim, Kim and Park. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Park, Jinhee Jeong, Kiho Kim, Manho Kim, Wijin Park, Ju Hyun Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein |
title | Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein |
title_full | Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein |
title_fullStr | Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein |
title_full_unstemmed | Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein |
title_short | Enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30Kc19α–Lin28A protein |
title_sort | enhanced osteogenesis of human urine-derived stem cells by direct delivery of 30kc19α–lin28a protein |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10293758/ https://www.ncbi.nlm.nih.gov/pubmed/37383520 http://dx.doi.org/10.3389/fbioe.2023.1215087 |
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