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Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy
OBJECTIVE: Glomerular filtration rate (GFR) is a key indicator of renal function. In both clinical practice and pre-clinical research, serum levels of endogenous filtration markers, such as creatinine, are often used to estimate GFR. However, these markers often do not reflect minor changes in renal...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294388/ https://www.ncbi.nlm.nih.gov/pubmed/37365638 http://dx.doi.org/10.1186/s13104-023-06387-y |
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author | Jensen, Michael Schou de Araujo, Isabela Bastos Binotti Abreu Mutsaers, Henricus A.M. Nørregaard, Rikke |
author_facet | Jensen, Michael Schou de Araujo, Isabela Bastos Binotti Abreu Mutsaers, Henricus A.M. Nørregaard, Rikke |
author_sort | Jensen, Michael Schou |
collection | PubMed |
description | OBJECTIVE: Glomerular filtration rate (GFR) is a key indicator of renal function. In both clinical practice and pre-clinical research, serum levels of endogenous filtration markers, such as creatinine, are often used to estimate GFR. However, these markers often do not reflect minor changes in renal function. In this study, we therefore set out to evaluate the applicability of transcutaneous GFR (tGFR) measurements to monitor the changes in renal function, as compared to plasma creatinine (pCreatinine), in two models of obstructive nephropathy, namely unilateral ureteral obstruction (UUO) or bilateral ureteral obstruction followed by release (BUO-R) in male Wistar rats. RESULTS: UUO animals showed a significant reduction in tGFR compared to baseline; whereas pCreatinine levels were not significantly changed. In BUO animals, tGFR drops 24 h post BUO and remains lower upon release of the obstruction until day 11. Concomitantly, pCreatinine levels were also increased 24 h after obstruction and 24 h post release, however after 4 days, pCreatinine returned to baseline levels. In conclusion, this study revealed that the tGFR method is superior at detecting minor changes in renal function as compared to pCreatinine measurements. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-023-06387-y. |
format | Online Article Text |
id | pubmed-10294388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102943882023-06-28 Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy Jensen, Michael Schou de Araujo, Isabela Bastos Binotti Abreu Mutsaers, Henricus A.M. Nørregaard, Rikke BMC Res Notes Research Note OBJECTIVE: Glomerular filtration rate (GFR) is a key indicator of renal function. In both clinical practice and pre-clinical research, serum levels of endogenous filtration markers, such as creatinine, are often used to estimate GFR. However, these markers often do not reflect minor changes in renal function. In this study, we therefore set out to evaluate the applicability of transcutaneous GFR (tGFR) measurements to monitor the changes in renal function, as compared to plasma creatinine (pCreatinine), in two models of obstructive nephropathy, namely unilateral ureteral obstruction (UUO) or bilateral ureteral obstruction followed by release (BUO-R) in male Wistar rats. RESULTS: UUO animals showed a significant reduction in tGFR compared to baseline; whereas pCreatinine levels were not significantly changed. In BUO animals, tGFR drops 24 h post BUO and remains lower upon release of the obstruction until day 11. Concomitantly, pCreatinine levels were also increased 24 h after obstruction and 24 h post release, however after 4 days, pCreatinine returned to baseline levels. In conclusion, this study revealed that the tGFR method is superior at detecting minor changes in renal function as compared to pCreatinine measurements. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-023-06387-y. BioMed Central 2023-06-26 /pmc/articles/PMC10294388/ /pubmed/37365638 http://dx.doi.org/10.1186/s13104-023-06387-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Jensen, Michael Schou de Araujo, Isabela Bastos Binotti Abreu Mutsaers, Henricus A.M. Nørregaard, Rikke Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy |
title | Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy |
title_full | Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy |
title_fullStr | Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy |
title_full_unstemmed | Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy |
title_short | Transcutaneous measurement of renal function in two rodent models of obstructive nephropathy |
title_sort | transcutaneous measurement of renal function in two rodent models of obstructive nephropathy |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294388/ https://www.ncbi.nlm.nih.gov/pubmed/37365638 http://dx.doi.org/10.1186/s13104-023-06387-y |
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