Cargando…

Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle

INTRODUCTION: The objectives of this study were to evaluate the impacts of two modified-live virus (MLV) vaccination protocols and respiratory disease (BRD) occurrence on the microbial community composition of the nasopharynx in feedlot cattle. METHODS: The treatment groups included in this randomiz...

Descripción completa

Detalles Bibliográficos
Autores principales: McAtee, Taylor B., Pinnell, Lee J., Powledge, Sherri A., Wolfe, Cory A., Morley, Paul S., Richeson, John T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294429/
https://www.ncbi.nlm.nih.gov/pubmed/37383638
http://dx.doi.org/10.3389/fmicb.2023.1203498
_version_ 1785063194989953024
author McAtee, Taylor B.
Pinnell, Lee J.
Powledge, Sherri A.
Wolfe, Cory A.
Morley, Paul S.
Richeson, John T.
author_facet McAtee, Taylor B.
Pinnell, Lee J.
Powledge, Sherri A.
Wolfe, Cory A.
Morley, Paul S.
Richeson, John T.
author_sort McAtee, Taylor B.
collection PubMed
description INTRODUCTION: The objectives of this study were to evaluate the impacts of two modified-live virus (MLV) vaccination protocols and respiratory disease (BRD) occurrence on the microbial community composition of the nasopharynx in feedlot cattle. METHODS: The treatment groups included in this randomized controlled trial included: 1) no viral respiratory vaccination (CON), 2) intranasal, trivalent, MLV respiratory vaccine in addition to a parenteral BVDV type I and II vaccine (INT), and 3) parenteral, pentavalent, MLV respiratory vaccination against the same agents (INJ). Calves (n = 525) arrived in 5 truckload blocks and were stratified by body weight, sex, and presence of a pre-existing identification ear-tag. A total of 600 nasal swab samples were selected for DNA extraction and subsequent 16S rRNA gene sequencing to characterize the microbiome of the upper respiratory tract. Nasal swabs collected on d 28 from healthy cattle were used to evaluate the impact of vaccination on upper respiratory tract (URT) microbial communities. RESULTS: Firmicutes were less abundant in INT calves (n = 114; P < 0.05) and this difference was attributed to decreased relative abundance (RA) of Mycoplasma spp. (P = 0.04). Mannheimia and Pasteurella had lower RA in INT (P < 0.05). The microbiome in healthy animals on d 28 had increased Proteobacteria (largely Moraxella spp.) and decreased Firmicutes (comprised almost exclusively of Mycoplasma spp.) compared to animals that were treated for or died from BRD (P < 0.05). Cattle that died had a greater RA of Mycoplasma spp. in their respiratory microbiome on d 0 (P < 0.02). Richness was similar on d 0 and 28, but diversity increased for all animals on d 28 (P>0.05).
format Online
Article
Text
id pubmed-10294429
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102944292023-06-28 Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle McAtee, Taylor B. Pinnell, Lee J. Powledge, Sherri A. Wolfe, Cory A. Morley, Paul S. Richeson, John T. Front Microbiol Microbiology INTRODUCTION: The objectives of this study were to evaluate the impacts of two modified-live virus (MLV) vaccination protocols and respiratory disease (BRD) occurrence on the microbial community composition of the nasopharynx in feedlot cattle. METHODS: The treatment groups included in this randomized controlled trial included: 1) no viral respiratory vaccination (CON), 2) intranasal, trivalent, MLV respiratory vaccine in addition to a parenteral BVDV type I and II vaccine (INT), and 3) parenteral, pentavalent, MLV respiratory vaccination against the same agents (INJ). Calves (n = 525) arrived in 5 truckload blocks and were stratified by body weight, sex, and presence of a pre-existing identification ear-tag. A total of 600 nasal swab samples were selected for DNA extraction and subsequent 16S rRNA gene sequencing to characterize the microbiome of the upper respiratory tract. Nasal swabs collected on d 28 from healthy cattle were used to evaluate the impact of vaccination on upper respiratory tract (URT) microbial communities. RESULTS: Firmicutes were less abundant in INT calves (n = 114; P < 0.05) and this difference was attributed to decreased relative abundance (RA) of Mycoplasma spp. (P = 0.04). Mannheimia and Pasteurella had lower RA in INT (P < 0.05). The microbiome in healthy animals on d 28 had increased Proteobacteria (largely Moraxella spp.) and decreased Firmicutes (comprised almost exclusively of Mycoplasma spp.) compared to animals that were treated for or died from BRD (P < 0.05). Cattle that died had a greater RA of Mycoplasma spp. in their respiratory microbiome on d 0 (P < 0.02). Richness was similar on d 0 and 28, but diversity increased for all animals on d 28 (P>0.05). Frontiers Media S.A. 2023-06-13 /pmc/articles/PMC10294429/ /pubmed/37383638 http://dx.doi.org/10.3389/fmicb.2023.1203498 Text en Copyright © 2023 McAtee, Pinnell, Powledge, Wolfe, Morley and Richeson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
McAtee, Taylor B.
Pinnell, Lee J.
Powledge, Sherri A.
Wolfe, Cory A.
Morley, Paul S.
Richeson, John T.
Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle
title Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle
title_full Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle
title_fullStr Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle
title_full_unstemmed Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle
title_short Effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle
title_sort effects of respiratory virus vaccination and bovine respiratory disease on the respiratory microbiome of feedlot cattle
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294429/
https://www.ncbi.nlm.nih.gov/pubmed/37383638
http://dx.doi.org/10.3389/fmicb.2023.1203498
work_keys_str_mv AT mcateetaylorb effectsofrespiratoryvirusvaccinationandbovinerespiratorydiseaseontherespiratorymicrobiomeoffeedlotcattle
AT pinnellleej effectsofrespiratoryvirusvaccinationandbovinerespiratorydiseaseontherespiratorymicrobiomeoffeedlotcattle
AT powledgesherria effectsofrespiratoryvirusvaccinationandbovinerespiratorydiseaseontherespiratorymicrobiomeoffeedlotcattle
AT wolfecorya effectsofrespiratoryvirusvaccinationandbovinerespiratorydiseaseontherespiratorymicrobiomeoffeedlotcattle
AT morleypauls effectsofrespiratoryvirusvaccinationandbovinerespiratorydiseaseontherespiratorymicrobiomeoffeedlotcattle
AT richesonjohnt effectsofrespiratoryvirusvaccinationandbovinerespiratorydiseaseontherespiratorymicrobiomeoffeedlotcattle