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Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation
Cells stabilize intracellular inorganic phosphate (P(i)) to compromise between large biosynthetic needs and detrimental bioenergetic effects of P(i). P(i) homeostasis in eukaryotes uses Syg1/Pho81/Xpr1 (SPX) domains, which are receptors for inositol pyrophosphates. We explored how polymerization and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Society for Microbiology
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294617/ https://www.ncbi.nlm.nih.gov/pubmed/37074217 http://dx.doi.org/10.1128/mbio.00102-23 |
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author | Kim, Geun-Don Qiu, Danye Jessen, Henning Jacob Mayer, Andreas |
author_facet | Kim, Geun-Don Qiu, Danye Jessen, Henning Jacob Mayer, Andreas |
author_sort | Kim, Geun-Don |
collection | PubMed |
description | Cells stabilize intracellular inorganic phosphate (P(i)) to compromise between large biosynthetic needs and detrimental bioenergetic effects of P(i). P(i) homeostasis in eukaryotes uses Syg1/Pho81/Xpr1 (SPX) domains, which are receptors for inositol pyrophosphates. We explored how polymerization and storage of P(i) in acidocalcisome-like vacuoles supports Saccharomyces cerevisiae metabolism and how these cells recognize P(i) scarcity. Whereas P(i) starvation affects numerous metabolic pathways, beginning P(i) scarcity affects few metabolites. These include inositol pyrophosphates and ATP, a low-affinity substrate for inositol pyrophosphate-synthesizing kinases. Declining ATP and inositol pyrophosphates may thus be indicators of impending P(i) limitation. Actual P(i) starvation triggers accumulation of the purine synthesis intermediate 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), which activates P(i)-dependent transcription factors. Cells lacking inorganic polyphosphate show P(i) starvation features already under P(i)-replete conditions, suggesting that vacuolar polyphosphate supplies P(i) for metabolism even when P(i) is abundant. However, polyphosphate deficiency also generates unique metabolic changes that are not observed in starving wild-type cells. Polyphosphate in acidocalcisome-like vacuoles may hence be more than a global phosphate reserve and channel P(i) to preferred cellular processes. |
format | Online Article Text |
id | pubmed-10294617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-102946172023-06-28 Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation Kim, Geun-Don Qiu, Danye Jessen, Henning Jacob Mayer, Andreas mBio Research Article Cells stabilize intracellular inorganic phosphate (P(i)) to compromise between large biosynthetic needs and detrimental bioenergetic effects of P(i). P(i) homeostasis in eukaryotes uses Syg1/Pho81/Xpr1 (SPX) domains, which are receptors for inositol pyrophosphates. We explored how polymerization and storage of P(i) in acidocalcisome-like vacuoles supports Saccharomyces cerevisiae metabolism and how these cells recognize P(i) scarcity. Whereas P(i) starvation affects numerous metabolic pathways, beginning P(i) scarcity affects few metabolites. These include inositol pyrophosphates and ATP, a low-affinity substrate for inositol pyrophosphate-synthesizing kinases. Declining ATP and inositol pyrophosphates may thus be indicators of impending P(i) limitation. Actual P(i) starvation triggers accumulation of the purine synthesis intermediate 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), which activates P(i)-dependent transcription factors. Cells lacking inorganic polyphosphate show P(i) starvation features already under P(i)-replete conditions, suggesting that vacuolar polyphosphate supplies P(i) for metabolism even when P(i) is abundant. However, polyphosphate deficiency also generates unique metabolic changes that are not observed in starving wild-type cells. Polyphosphate in acidocalcisome-like vacuoles may hence be more than a global phosphate reserve and channel P(i) to preferred cellular processes. American Society for Microbiology 2023-04-19 /pmc/articles/PMC10294617/ /pubmed/37074217 http://dx.doi.org/10.1128/mbio.00102-23 Text en Copyright © 2023 Kim et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Kim, Geun-Don Qiu, Danye Jessen, Henning Jacob Mayer, Andreas Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation |
title | Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation |
title_full | Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation |
title_fullStr | Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation |
title_full_unstemmed | Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation |
title_short | Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation |
title_sort | metabolic consequences of polyphosphate synthesis and imminent phosphate limitation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294617/ https://www.ncbi.nlm.nih.gov/pubmed/37074217 http://dx.doi.org/10.1128/mbio.00102-23 |
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