Cargando…
Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting
Layered Double Hydroxides (LDHs) are inorganic compounds of relevance to various domains, where their surface reactivity and/or intercalation capacities can be advantageously exploited for the retention/release of ionic and molecular species. In this study, we have explored specifically the applicab...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294808/ https://www.ncbi.nlm.nih.gov/pubmed/37370665 http://dx.doi.org/10.3390/bioengineering10060734 |
_version_ | 1785063270883786752 |
---|---|
author | Kim, Hyoung-Jun Lagarrigue, Prescillia Oh, Jae-Min Soulié, Jérémy Salles, Fabrice Cazalbou, Sophie Drouet, Christophe |
author_facet | Kim, Hyoung-Jun Lagarrigue, Prescillia Oh, Jae-Min Soulié, Jérémy Salles, Fabrice Cazalbou, Sophie Drouet, Christophe |
author_sort | Kim, Hyoung-Jun |
collection | PubMed |
description | Layered Double Hydroxides (LDHs) are inorganic compounds of relevance to various domains, where their surface reactivity and/or intercalation capacities can be advantageously exploited for the retention/release of ionic and molecular species. In this study, we have explored specifically the applicability in the field of bone regeneration of one LDH composition, denoted “MgFeCO(3)”, of which components are already present in vivo, so as to convey a biocompatibility character. The propensity to be used as a bone substitute depends, however, on their ability to allow the fabrication of 3D constructs able to be implanted in bone sites. In this work, we display two appealing approaches for the processing of MgFeCO(3) LDH particles to prepare (i) porous 3D scaffolds by freeze-casting, involving an alginate biopolymeric matrix, and (ii) pure MgFeCO(3) LDH monoliths by Spark Plasma Sintering (SPS) at low temperature. We then explored the capacity of such LDH particles or monoliths to interact quantitatively with molecular moieties/drugs in view of their local release. The experimental data were complemented by computational chemistry calculations (Monte Carlo) to examine in more detail the mineral–organic interactions at play. Finally, preliminary in vitro tests on osteoblastic MG63 cells confirmed the high biocompatible character of this LDH composition. It was confirmed that (i) thermodynamically metastable LDH could be successfully consolidated into a monolith through SPS, (ii) the LDH particles could be incorporated into a polymer matrix through freeze casting, and (iii) the LDH in the consolidated monolith could incorporate and release drug molecules in a controlled manner. In other words, our results indicate that the MgFeCO(3) LDH (pyroaurite structure) may be seen as a new promising compound for the setup of bone substitute biomaterials with tailorable drug delivery capacity, including for personalized medicine. |
format | Online Article Text |
id | pubmed-10294808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102948082023-06-28 Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting Kim, Hyoung-Jun Lagarrigue, Prescillia Oh, Jae-Min Soulié, Jérémy Salles, Fabrice Cazalbou, Sophie Drouet, Christophe Bioengineering (Basel) Article Layered Double Hydroxides (LDHs) are inorganic compounds of relevance to various domains, where their surface reactivity and/or intercalation capacities can be advantageously exploited for the retention/release of ionic and molecular species. In this study, we have explored specifically the applicability in the field of bone regeneration of one LDH composition, denoted “MgFeCO(3)”, of which components are already present in vivo, so as to convey a biocompatibility character. The propensity to be used as a bone substitute depends, however, on their ability to allow the fabrication of 3D constructs able to be implanted in bone sites. In this work, we display two appealing approaches for the processing of MgFeCO(3) LDH particles to prepare (i) porous 3D scaffolds by freeze-casting, involving an alginate biopolymeric matrix, and (ii) pure MgFeCO(3) LDH monoliths by Spark Plasma Sintering (SPS) at low temperature. We then explored the capacity of such LDH particles or monoliths to interact quantitatively with molecular moieties/drugs in view of their local release. The experimental data were complemented by computational chemistry calculations (Monte Carlo) to examine in more detail the mineral–organic interactions at play. Finally, preliminary in vitro tests on osteoblastic MG63 cells confirmed the high biocompatible character of this LDH composition. It was confirmed that (i) thermodynamically metastable LDH could be successfully consolidated into a monolith through SPS, (ii) the LDH particles could be incorporated into a polymer matrix through freeze casting, and (iii) the LDH in the consolidated monolith could incorporate and release drug molecules in a controlled manner. In other words, our results indicate that the MgFeCO(3) LDH (pyroaurite structure) may be seen as a new promising compound for the setup of bone substitute biomaterials with tailorable drug delivery capacity, including for personalized medicine. MDPI 2023-06-19 /pmc/articles/PMC10294808/ /pubmed/37370665 http://dx.doi.org/10.3390/bioengineering10060734 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Hyoung-Jun Lagarrigue, Prescillia Oh, Jae-Min Soulié, Jérémy Salles, Fabrice Cazalbou, Sophie Drouet, Christophe Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
title | Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
title_full | Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
title_fullStr | Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
title_full_unstemmed | Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
title_short | Biocompatible MgFeCO(3) Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
title_sort | biocompatible mgfeco(3) layered double hydroxide (ldh) for bone regeneration—low-temperature processing through cold sintering and freeze-casting |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294808/ https://www.ncbi.nlm.nih.gov/pubmed/37370665 http://dx.doi.org/10.3390/bioengineering10060734 |
work_keys_str_mv | AT kimhyoungjun biocompatiblemgfeco3layereddoublehydroxideldhforboneregenerationlowtemperatureprocessingthroughcoldsinteringandfreezecasting AT lagarrigueprescillia biocompatiblemgfeco3layereddoublehydroxideldhforboneregenerationlowtemperatureprocessingthroughcoldsinteringandfreezecasting AT ohjaemin biocompatiblemgfeco3layereddoublehydroxideldhforboneregenerationlowtemperatureprocessingthroughcoldsinteringandfreezecasting AT souliejeremy biocompatiblemgfeco3layereddoublehydroxideldhforboneregenerationlowtemperatureprocessingthroughcoldsinteringandfreezecasting AT sallesfabrice biocompatiblemgfeco3layereddoublehydroxideldhforboneregenerationlowtemperatureprocessingthroughcoldsinteringandfreezecasting AT cazalbousophie biocompatiblemgfeco3layereddoublehydroxideldhforboneregenerationlowtemperatureprocessingthroughcoldsinteringandfreezecasting AT drouetchristophe biocompatiblemgfeco3layereddoublehydroxideldhforboneregenerationlowtemperatureprocessingthroughcoldsinteringandfreezecasting |