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Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris

Cordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of Cordyceps militaris with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with...

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Autores principales: Turk, Ayman, Lee, Solip, Yeon, Sang Won, Ryu, Se Hwan, Han, Yoo Kyong, Kim, Young Jun, Ko, Sung Min, Kim, Beom Seok, Hwang, Bang Yeon, Lee, Ki Yong, Lee, Mi Kyeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294830/
https://www.ncbi.nlm.nih.gov/pubmed/37371990
http://dx.doi.org/10.3390/antiox12061260
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author Turk, Ayman
Lee, Solip
Yeon, Sang Won
Ryu, Se Hwan
Han, Yoo Kyong
Kim, Young Jun
Ko, Sung Min
Kim, Beom Seok
Hwang, Bang Yeon
Lee, Ki Yong
Lee, Mi Kyeong
author_facet Turk, Ayman
Lee, Solip
Yeon, Sang Won
Ryu, Se Hwan
Han, Yoo Kyong
Kim, Young Jun
Ko, Sung Min
Kim, Beom Seok
Hwang, Bang Yeon
Lee, Ki Yong
Lee, Mi Kyeong
author_sort Turk, Ayman
collection PubMed
description Cordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of Cordyceps militaris with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with cultivation substrates of Cordyceps to increase the cordycepin content. Cordyceps cultivated on brown rice supplemented with Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigantis Radix exhibited increased cordycepin content compared to a brown rice control. Among them, the addition of 25% Mori Folium increased the cordycepin content up to 4 times. Adenosine deaminase (ADA) modulates the deamination of adenosine and deoxyadenosine, and the inhibitors have therapeutic potential with anti-proliferative and anti-inflammatory properties. As ADA is also known to be involved in converting cordycepin to 3′-deoxyinosine, the inhibitory activity of medicinal plants on ADA was measured by spectrophotometric analysis using cordycepin as a substrate. As expected, Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigas Radix strongly inhibited ADA activity. Molecular docking analysis also showed the correlation between ADA and the major components of these medicinal plants. Conclusively, our research suggests a new strategy of using medicinal plants to enhance cordycepin production in C. militaris.
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spelling pubmed-102948302023-06-28 Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris Turk, Ayman Lee, Solip Yeon, Sang Won Ryu, Se Hwan Han, Yoo Kyong Kim, Young Jun Ko, Sung Min Kim, Beom Seok Hwang, Bang Yeon Lee, Ki Yong Lee, Mi Kyeong Antioxidants (Basel) Article Cordycepin, also known as 3′-deoxyadenosine, is a major active ingredient of Cordyceps militaris with diverse pharmacological effects. Due to its limited supply, many attempts have been conducted to enhance the cordycepin content. As part of this study, eight medicinal plants were supplemented with cultivation substrates of Cordyceps to increase the cordycepin content. Cordyceps cultivated on brown rice supplemented with Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigantis Radix exhibited increased cordycepin content compared to a brown rice control. Among them, the addition of 25% Mori Folium increased the cordycepin content up to 4 times. Adenosine deaminase (ADA) modulates the deamination of adenosine and deoxyadenosine, and the inhibitors have therapeutic potential with anti-proliferative and anti-inflammatory properties. As ADA is also known to be involved in converting cordycepin to 3′-deoxyinosine, the inhibitory activity of medicinal plants on ADA was measured by spectrophotometric analysis using cordycepin as a substrate. As expected, Mori Folium, Curcumae Rhizoma, Saururi Herba, and Angelicae Gigas Radix strongly inhibited ADA activity. Molecular docking analysis also showed the correlation between ADA and the major components of these medicinal plants. Conclusively, our research suggests a new strategy of using medicinal plants to enhance cordycepin production in C. militaris. MDPI 2023-06-12 /pmc/articles/PMC10294830/ /pubmed/37371990 http://dx.doi.org/10.3390/antiox12061260 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turk, Ayman
Lee, Solip
Yeon, Sang Won
Ryu, Se Hwan
Han, Yoo Kyong
Kim, Young Jun
Ko, Sung Min
Kim, Beom Seok
Hwang, Bang Yeon
Lee, Ki Yong
Lee, Mi Kyeong
Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
title Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
title_full Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
title_fullStr Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
title_full_unstemmed Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
title_short Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
title_sort adenosine deaminase inhibitory activity of medicinal plants: boost the production of cordycepin in cordyceps militaris
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294830/
https://www.ncbi.nlm.nih.gov/pubmed/37371990
http://dx.doi.org/10.3390/antiox12061260
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