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The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites
This study aimed to assess the bone regeneration of critical-size defects in rabbit calvaria filled with freshly crushed extracted teeth, comparing them with BTCP biomaterial and empty sites. Materials and methods: Twenty-one female New Zealand rabbits were used in this study. Two critical-size defe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294938/ https://www.ncbi.nlm.nih.gov/pubmed/37370569 http://dx.doi.org/10.3390/bioengineering10060638 |
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author | Calvo-Guirado, José Luis Cabo-Pastor, Marta Belén Martínez-Martínez, Francisco Garcés-Villalá, Miguel Ángel de Carlos-Villafranca, Félix García-Carrillo, Nuria Fernández-Domínguez, Manuel |
author_facet | Calvo-Guirado, José Luis Cabo-Pastor, Marta Belén Martínez-Martínez, Francisco Garcés-Villalá, Miguel Ángel de Carlos-Villafranca, Félix García-Carrillo, Nuria Fernández-Domínguez, Manuel |
author_sort | Calvo-Guirado, José Luis |
collection | PubMed |
description | This study aimed to assess the bone regeneration of critical-size defects in rabbit calvaria filled with freshly crushed extracted teeth, comparing them with BTCP biomaterial and empty sites. Materials and methods: Twenty-one female New Zealand rabbits were used in this study. Two critical-size defects 6 mm in size were created in the skull bone, each with a 3 mm separation between them. Three experimental groups were evaluated: Group A (human sterilized crushed teeth granules alone), Group B (Bioner Bone, Bioner Sitemas Implantológicos), and Group C (unfilled defects). The animals were sacrificed at 4 and 8 weeks. Evaluation of the samples involved histological and histomorphometric analyses with radiographic evaluation. The histological evaluation showed a higher volume reduction in Group A compared with Group B (p < 0.05) and Control. Group A showed the highest values for cortical closure and bone formation around the particles, followed by Group B and Group C (p < 0.05). Within the limitations of this animal study, we can conclude that the use of human tooth particles leads to increased bone formation and reduced connective tissue in critical-size defects in rabbit calvaria when compared to BTCP biomaterial. The calvarial model is a robust base for the evaluation of different biomaterials. |
format | Online Article Text |
id | pubmed-10294938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102949382023-06-28 The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites Calvo-Guirado, José Luis Cabo-Pastor, Marta Belén Martínez-Martínez, Francisco Garcés-Villalá, Miguel Ángel de Carlos-Villafranca, Félix García-Carrillo, Nuria Fernández-Domínguez, Manuel Bioengineering (Basel) Article This study aimed to assess the bone regeneration of critical-size defects in rabbit calvaria filled with freshly crushed extracted teeth, comparing them with BTCP biomaterial and empty sites. Materials and methods: Twenty-one female New Zealand rabbits were used in this study. Two critical-size defects 6 mm in size were created in the skull bone, each with a 3 mm separation between them. Three experimental groups were evaluated: Group A (human sterilized crushed teeth granules alone), Group B (Bioner Bone, Bioner Sitemas Implantológicos), and Group C (unfilled defects). The animals were sacrificed at 4 and 8 weeks. Evaluation of the samples involved histological and histomorphometric analyses with radiographic evaluation. The histological evaluation showed a higher volume reduction in Group A compared with Group B (p < 0.05) and Control. Group A showed the highest values for cortical closure and bone formation around the particles, followed by Group B and Group C (p < 0.05). Within the limitations of this animal study, we can conclude that the use of human tooth particles leads to increased bone formation and reduced connective tissue in critical-size defects in rabbit calvaria when compared to BTCP biomaterial. The calvarial model is a robust base for the evaluation of different biomaterials. MDPI 2023-05-24 /pmc/articles/PMC10294938/ /pubmed/37370569 http://dx.doi.org/10.3390/bioengineering10060638 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Calvo-Guirado, José Luis Cabo-Pastor, Marta Belén Martínez-Martínez, Francisco Garcés-Villalá, Miguel Ángel de Carlos-Villafranca, Félix García-Carrillo, Nuria Fernández-Domínguez, Manuel The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites |
title | The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites |
title_full | The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites |
title_fullStr | The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites |
title_full_unstemmed | The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites |
title_short | The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites |
title_sort | use of human sterilized crushed tooth particles compared with btcp biomaterial and empty defects in bone formation inside critical rabbit calvaria sites |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294938/ https://www.ncbi.nlm.nih.gov/pubmed/37370569 http://dx.doi.org/10.3390/bioengineering10060638 |
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