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The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites

This study aimed to assess the bone regeneration of critical-size defects in rabbit calvaria filled with freshly crushed extracted teeth, comparing them with BTCP biomaterial and empty sites. Materials and methods: Twenty-one female New Zealand rabbits were used in this study. Two critical-size defe...

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Autores principales: Calvo-Guirado, José Luis, Cabo-Pastor, Marta Belén, Martínez-Martínez, Francisco, Garcés-Villalá, Miguel Ángel, de Carlos-Villafranca, Félix, García-Carrillo, Nuria, Fernández-Domínguez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294938/
https://www.ncbi.nlm.nih.gov/pubmed/37370569
http://dx.doi.org/10.3390/bioengineering10060638
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author Calvo-Guirado, José Luis
Cabo-Pastor, Marta Belén
Martínez-Martínez, Francisco
Garcés-Villalá, Miguel Ángel
de Carlos-Villafranca, Félix
García-Carrillo, Nuria
Fernández-Domínguez, Manuel
author_facet Calvo-Guirado, José Luis
Cabo-Pastor, Marta Belén
Martínez-Martínez, Francisco
Garcés-Villalá, Miguel Ángel
de Carlos-Villafranca, Félix
García-Carrillo, Nuria
Fernández-Domínguez, Manuel
author_sort Calvo-Guirado, José Luis
collection PubMed
description This study aimed to assess the bone regeneration of critical-size defects in rabbit calvaria filled with freshly crushed extracted teeth, comparing them with BTCP biomaterial and empty sites. Materials and methods: Twenty-one female New Zealand rabbits were used in this study. Two critical-size defects 6 mm in size were created in the skull bone, each with a 3 mm separation between them. Three experimental groups were evaluated: Group A (human sterilized crushed teeth granules alone), Group B (Bioner Bone, Bioner Sitemas Implantológicos), and Group C (unfilled defects). The animals were sacrificed at 4 and 8 weeks. Evaluation of the samples involved histological and histomorphometric analyses with radiographic evaluation. The histological evaluation showed a higher volume reduction in Group A compared with Group B (p < 0.05) and Control. Group A showed the highest values for cortical closure and bone formation around the particles, followed by Group B and Group C (p < 0.05). Within the limitations of this animal study, we can conclude that the use of human tooth particles leads to increased bone formation and reduced connective tissue in critical-size defects in rabbit calvaria when compared to BTCP biomaterial. The calvarial model is a robust base for the evaluation of different biomaterials.
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spelling pubmed-102949382023-06-28 The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites Calvo-Guirado, José Luis Cabo-Pastor, Marta Belén Martínez-Martínez, Francisco Garcés-Villalá, Miguel Ángel de Carlos-Villafranca, Félix García-Carrillo, Nuria Fernández-Domínguez, Manuel Bioengineering (Basel) Article This study aimed to assess the bone regeneration of critical-size defects in rabbit calvaria filled with freshly crushed extracted teeth, comparing them with BTCP biomaterial and empty sites. Materials and methods: Twenty-one female New Zealand rabbits were used in this study. Two critical-size defects 6 mm in size were created in the skull bone, each with a 3 mm separation between them. Three experimental groups were evaluated: Group A (human sterilized crushed teeth granules alone), Group B (Bioner Bone, Bioner Sitemas Implantológicos), and Group C (unfilled defects). The animals were sacrificed at 4 and 8 weeks. Evaluation of the samples involved histological and histomorphometric analyses with radiographic evaluation. The histological evaluation showed a higher volume reduction in Group A compared with Group B (p < 0.05) and Control. Group A showed the highest values for cortical closure and bone formation around the particles, followed by Group B and Group C (p < 0.05). Within the limitations of this animal study, we can conclude that the use of human tooth particles leads to increased bone formation and reduced connective tissue in critical-size defects in rabbit calvaria when compared to BTCP biomaterial. The calvarial model is a robust base for the evaluation of different biomaterials. MDPI 2023-05-24 /pmc/articles/PMC10294938/ /pubmed/37370569 http://dx.doi.org/10.3390/bioengineering10060638 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Calvo-Guirado, José Luis
Cabo-Pastor, Marta Belén
Martínez-Martínez, Francisco
Garcés-Villalá, Miguel Ángel
de Carlos-Villafranca, Félix
García-Carrillo, Nuria
Fernández-Domínguez, Manuel
The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites
title The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites
title_full The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites
title_fullStr The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites
title_full_unstemmed The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites
title_short The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites
title_sort use of human sterilized crushed tooth particles compared with btcp biomaterial and empty defects in bone formation inside critical rabbit calvaria sites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294938/
https://www.ncbi.nlm.nih.gov/pubmed/37370569
http://dx.doi.org/10.3390/bioengineering10060638
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