Cargando…

Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice

Systemic lupus erythematosus (SLE) is a chronic immune-inflammatory disease characterized by multiorgan affectation and lowered self-tolerance. Additionally, epigenetic changes have been described as playing a pivotal role in SLE. This work aims to assess the effects of oleacein (OLA), one of the ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Muñoz-García, Rocío, Sánchez-Hidalgo, Marina, Alcarranza, Manuel, Vazquéz-Román, María Victoria, de Sotomayor, María Alvarez, González-Rodríguez, María Luisa, de Andrés, María C., Alarcón-de-la-Lastra, Catalina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294986/
https://www.ncbi.nlm.nih.gov/pubmed/37372034
http://dx.doi.org/10.3390/antiox12061303
_version_ 1785063313101553664
author Muñoz-García, Rocío
Sánchez-Hidalgo, Marina
Alcarranza, Manuel
Vazquéz-Román, María Victoria
de Sotomayor, María Alvarez
González-Rodríguez, María Luisa
de Andrés, María C.
Alarcón-de-la-Lastra, Catalina
author_facet Muñoz-García, Rocío
Sánchez-Hidalgo, Marina
Alcarranza, Manuel
Vazquéz-Román, María Victoria
de Sotomayor, María Alvarez
González-Rodríguez, María Luisa
de Andrés, María C.
Alarcón-de-la-Lastra, Catalina
author_sort Muñoz-García, Rocío
collection PubMed
description Systemic lupus erythematosus (SLE) is a chronic immune-inflammatory disease characterized by multiorgan affectation and lowered self-tolerance. Additionally, epigenetic changes have been described as playing a pivotal role in SLE. This work aims to assess the effects of oleacein (OLA), one of the main extra virgin olive oil secoiridoids, when used to supplement the diet of a murine pristane-induced SLE model. In the study, 12-week-old female BALB/c mice were injected with pristane and fed with an OLA-enriched diet (0.01 % (w/w)) for 24 weeks. The presence of immune complexes was evaluated by immunohistochemistry and immunofluorescence. Endothelial dysfunction was studied in thoracic aortas. Signaling pathways and oxidative-inflammatory-related mediators were evaluated by Western blotting. Moreover, we studied epigenetic changes such as DNA methyltransferase (DNMT-1) and micro(mi)RNAs expression in renal tissue. Nutritional treatment with OLA reduced the deposition of immune complexes, ameliorating kidney damage. These protective effects could be related to the modulation of mitogen-activated protein kinases, the Janus kinase/signal transducer and transcription activator of transcription, nuclear factor kappa, nuclear-factor-erythroid-2-related factor 2, inflammasome signaling pathways, and the regulation of miRNAs (miRNA-126, miRNA-146a, miRNA-24-3p, and miRNA-123) and DNMT-1 expression. Moreover, the OLA-enriched diet normalized endothelial nitric oxide synthase and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-1 overexpression. These preliminary results suggest that an OLA-supplemented diet could constitute a new alternative nutraceutical therapy in the management of SLE, supporting this compound as a novel epigenetic modulator of the immunoinflammatory response.
format Online
Article
Text
id pubmed-10294986
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102949862023-06-28 Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice Muñoz-García, Rocío Sánchez-Hidalgo, Marina Alcarranza, Manuel Vazquéz-Román, María Victoria de Sotomayor, María Alvarez González-Rodríguez, María Luisa de Andrés, María C. Alarcón-de-la-Lastra, Catalina Antioxidants (Basel) Article Systemic lupus erythematosus (SLE) is a chronic immune-inflammatory disease characterized by multiorgan affectation and lowered self-tolerance. Additionally, epigenetic changes have been described as playing a pivotal role in SLE. This work aims to assess the effects of oleacein (OLA), one of the main extra virgin olive oil secoiridoids, when used to supplement the diet of a murine pristane-induced SLE model. In the study, 12-week-old female BALB/c mice were injected with pristane and fed with an OLA-enriched diet (0.01 % (w/w)) for 24 weeks. The presence of immune complexes was evaluated by immunohistochemistry and immunofluorescence. Endothelial dysfunction was studied in thoracic aortas. Signaling pathways and oxidative-inflammatory-related mediators were evaluated by Western blotting. Moreover, we studied epigenetic changes such as DNA methyltransferase (DNMT-1) and micro(mi)RNAs expression in renal tissue. Nutritional treatment with OLA reduced the deposition of immune complexes, ameliorating kidney damage. These protective effects could be related to the modulation of mitogen-activated protein kinases, the Janus kinase/signal transducer and transcription activator of transcription, nuclear factor kappa, nuclear-factor-erythroid-2-related factor 2, inflammasome signaling pathways, and the regulation of miRNAs (miRNA-126, miRNA-146a, miRNA-24-3p, and miRNA-123) and DNMT-1 expression. Moreover, the OLA-enriched diet normalized endothelial nitric oxide synthase and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-1 overexpression. These preliminary results suggest that an OLA-supplemented diet could constitute a new alternative nutraceutical therapy in the management of SLE, supporting this compound as a novel epigenetic modulator of the immunoinflammatory response. MDPI 2023-06-19 /pmc/articles/PMC10294986/ /pubmed/37372034 http://dx.doi.org/10.3390/antiox12061303 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muñoz-García, Rocío
Sánchez-Hidalgo, Marina
Alcarranza, Manuel
Vazquéz-Román, María Victoria
de Sotomayor, María Alvarez
González-Rodríguez, María Luisa
de Andrés, María C.
Alarcón-de-la-Lastra, Catalina
Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice
title Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice
title_full Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice
title_fullStr Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice
title_full_unstemmed Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice
title_short Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice
title_sort effects of dietary oleacein treatment on endothelial dysfunction and lupus nephritis in balb/c pristane-induced mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294986/
https://www.ncbi.nlm.nih.gov/pubmed/37372034
http://dx.doi.org/10.3390/antiox12061303
work_keys_str_mv AT munozgarciarocio effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice
AT sanchezhidalgomarina effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice
AT alcarranzamanuel effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice
AT vazquezromanmariavictoria effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice
AT desotomayormariaalvarez effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice
AT gonzalezrodriguezmarialuisa effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice
AT deandresmariac effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice
AT alarcondelalastracatalina effectsofdietaryoleaceintreatmentonendothelialdysfunctionandlupusnephritisinbalbcpristaneinducedmice