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Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity

This study aimed to examine the effects of a 14-day treatment with lady’s bedstraw methanol extract on doxorubicin-induced cardiotoxicity through functional, biochemical and histological examinations. We used 24 male Wistar albino rats divided into the following groups: control (CTRL), doxorubicin (...

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Autores principales: Bradic, Jovana, Andjic, Marijana, Novakovic, Jovana, Kocovic, Aleksandar, Tomovic, Marina, Petrovic, Anica, Nikolic, Marina, Mitrovic, Slobodanka, Jakovljevic, Vladimir, Pecarski, Danijela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295214/
https://www.ncbi.nlm.nih.gov/pubmed/37372007
http://dx.doi.org/10.3390/antiox12061277
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author Bradic, Jovana
Andjic, Marijana
Novakovic, Jovana
Kocovic, Aleksandar
Tomovic, Marina
Petrovic, Anica
Nikolic, Marina
Mitrovic, Slobodanka
Jakovljevic, Vladimir
Pecarski, Danijela
author_facet Bradic, Jovana
Andjic, Marijana
Novakovic, Jovana
Kocovic, Aleksandar
Tomovic, Marina
Petrovic, Anica
Nikolic, Marina
Mitrovic, Slobodanka
Jakovljevic, Vladimir
Pecarski, Danijela
author_sort Bradic, Jovana
collection PubMed
description This study aimed to examine the effects of a 14-day treatment with lady’s bedstraw methanol extract on doxorubicin-induced cardiotoxicity through functional, biochemical and histological examinations. We used 24 male Wistar albino rats divided into the following groups: control (CTRL), doxorubicin (DOX), and DOX + GVE (Galium verum extract). GVE was administered orally at a dose of 50 mg/kg per day for 14 days, while a single dose of doxorubicin was injected into the DOX groups. After accomplishing treatment with GVE, cardiac function was assessed, which determined the redox state. During the autoregulation protocol on the Langendorff apparatus, ex vivo cardiodynamic parameters were measured. Our results demonstrated that the consumption of GVE effectively suppressed the disturbed response of the heart to changes in perfusion pressures caused by administration of DOX. Intake of GVE was associated with a reduction in most of the measured prooxidants in comparison to the DOX group. Moreover, this extract was capable of increasing the activity of the antioxidant defense system. Morphometric analyses showed that rat hearts treated with DOX showed more pronounced degenerative changes and necrosis compared to the CTRL group. However, GVE pretreatment seems to be able to prevent the pathological injuries caused by DOX injection via decrease in oxidative stress and apoptosis.
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spelling pubmed-102952142023-06-28 Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity Bradic, Jovana Andjic, Marijana Novakovic, Jovana Kocovic, Aleksandar Tomovic, Marina Petrovic, Anica Nikolic, Marina Mitrovic, Slobodanka Jakovljevic, Vladimir Pecarski, Danijela Antioxidants (Basel) Article This study aimed to examine the effects of a 14-day treatment with lady’s bedstraw methanol extract on doxorubicin-induced cardiotoxicity through functional, biochemical and histological examinations. We used 24 male Wistar albino rats divided into the following groups: control (CTRL), doxorubicin (DOX), and DOX + GVE (Galium verum extract). GVE was administered orally at a dose of 50 mg/kg per day for 14 days, while a single dose of doxorubicin was injected into the DOX groups. After accomplishing treatment with GVE, cardiac function was assessed, which determined the redox state. During the autoregulation protocol on the Langendorff apparatus, ex vivo cardiodynamic parameters were measured. Our results demonstrated that the consumption of GVE effectively suppressed the disturbed response of the heart to changes in perfusion pressures caused by administration of DOX. Intake of GVE was associated with a reduction in most of the measured prooxidants in comparison to the DOX group. Moreover, this extract was capable of increasing the activity of the antioxidant defense system. Morphometric analyses showed that rat hearts treated with DOX showed more pronounced degenerative changes and necrosis compared to the CTRL group. However, GVE pretreatment seems to be able to prevent the pathological injuries caused by DOX injection via decrease in oxidative stress and apoptosis. MDPI 2023-06-15 /pmc/articles/PMC10295214/ /pubmed/37372007 http://dx.doi.org/10.3390/antiox12061277 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bradic, Jovana
Andjic, Marijana
Novakovic, Jovana
Kocovic, Aleksandar
Tomovic, Marina
Petrovic, Anica
Nikolic, Marina
Mitrovic, Slobodanka
Jakovljevic, Vladimir
Pecarski, Danijela
Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity
title Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity
title_full Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity
title_fullStr Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity
title_full_unstemmed Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity
title_short Lady’s Bedstraw as a Powerful Antioxidant for Attenuation of Doxorubicin-Induced Cardiotoxicity
title_sort lady’s bedstraw as a powerful antioxidant for attenuation of doxorubicin-induced cardiotoxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295214/
https://www.ncbi.nlm.nih.gov/pubmed/37372007
http://dx.doi.org/10.3390/antiox12061277
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