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Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model

Gelatin-based hydrogels have emerged as a popular scaffold material for tissue engineering applications. The introduction of variable crosslinking methods has shown promise for fabricating stable cell-laden scaffolds. In this work, we examine promising composite biopolymer-based inks for extrusion-b...

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Autores principales: Shehzad, Ahmer, Mukasheva, Fariza, Moazzam, Muhammad, Sultanova, Dana, Abdikhan, Birzhan, Trifonov, Alexander, Akilbekova, Dana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295226/
https://www.ncbi.nlm.nih.gov/pubmed/37370635
http://dx.doi.org/10.3390/bioengineering10060704
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author Shehzad, Ahmer
Mukasheva, Fariza
Moazzam, Muhammad
Sultanova, Dana
Abdikhan, Birzhan
Trifonov, Alexander
Akilbekova, Dana
author_facet Shehzad, Ahmer
Mukasheva, Fariza
Moazzam, Muhammad
Sultanova, Dana
Abdikhan, Birzhan
Trifonov, Alexander
Akilbekova, Dana
author_sort Shehzad, Ahmer
collection PubMed
description Gelatin-based hydrogels have emerged as a popular scaffold material for tissue engineering applications. The introduction of variable crosslinking methods has shown promise for fabricating stable cell-laden scaffolds. In this work, we examine promising composite biopolymer-based inks for extrusion-based 3D bioprinting, using a dual crosslinking approach. A combination of carefully selected printable hydrogel ink compositions and the use of photoinduced covalent and ionic crosslinking mechanisms allows for the fabrication of scaffolds of high accuracy and low cytotoxicity, resulting in unimpeded cell proliferation, extracellular matrix deposition, and mineralization. Three selected bioink compositions were characterized and the respective cell-laden scaffolds were bioprinted. Temporal stability, morphology, swelling, and mechanical properties of the scaffolds were thoroughly studied and the biocompatibility of the constructs was assessed using rat mesenchymal stem cells while focusing on osteogenesis. Experimental results showed that the composition of 1% alginate, 4% gelatin, and 5% (w/v) gelatine methacrylate, was found to be optimal among the examined, with shape fidelity of 88%, large cell spreading area and cell viability at around 100% after 14 days. The large pore diameters that exceed 100 µm, and highly interconnected scaffold morphology, make these hydrogels extremely potent in bone tissue engineering and bone organoid fabrication.
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spelling pubmed-102952262023-06-28 Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model Shehzad, Ahmer Mukasheva, Fariza Moazzam, Muhammad Sultanova, Dana Abdikhan, Birzhan Trifonov, Alexander Akilbekova, Dana Bioengineering (Basel) Article Gelatin-based hydrogels have emerged as a popular scaffold material for tissue engineering applications. The introduction of variable crosslinking methods has shown promise for fabricating stable cell-laden scaffolds. In this work, we examine promising composite biopolymer-based inks for extrusion-based 3D bioprinting, using a dual crosslinking approach. A combination of carefully selected printable hydrogel ink compositions and the use of photoinduced covalent and ionic crosslinking mechanisms allows for the fabrication of scaffolds of high accuracy and low cytotoxicity, resulting in unimpeded cell proliferation, extracellular matrix deposition, and mineralization. Three selected bioink compositions were characterized and the respective cell-laden scaffolds were bioprinted. Temporal stability, morphology, swelling, and mechanical properties of the scaffolds were thoroughly studied and the biocompatibility of the constructs was assessed using rat mesenchymal stem cells while focusing on osteogenesis. Experimental results showed that the composition of 1% alginate, 4% gelatin, and 5% (w/v) gelatine methacrylate, was found to be optimal among the examined, with shape fidelity of 88%, large cell spreading area and cell viability at around 100% after 14 days. The large pore diameters that exceed 100 µm, and highly interconnected scaffold morphology, make these hydrogels extremely potent in bone tissue engineering and bone organoid fabrication. MDPI 2023-06-09 /pmc/articles/PMC10295226/ /pubmed/37370635 http://dx.doi.org/10.3390/bioengineering10060704 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shehzad, Ahmer
Mukasheva, Fariza
Moazzam, Muhammad
Sultanova, Dana
Abdikhan, Birzhan
Trifonov, Alexander
Akilbekova, Dana
Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model
title Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model
title_full Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model
title_fullStr Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model
title_full_unstemmed Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model
title_short Dual-Crosslinking of Gelatin-Based Hydrogels: Promising Compositions for a 3D Printed Organotypic Bone Model
title_sort dual-crosslinking of gelatin-based hydrogels: promising compositions for a 3d printed organotypic bone model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295226/
https://www.ncbi.nlm.nih.gov/pubmed/37370635
http://dx.doi.org/10.3390/bioengineering10060704
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