A Review of Sperm Ultrastructural Characters in the Opecoelidae (Digenea) and Their Phylogenetic Implications, with New Data on Peracreadium characis, a Parasite of Diplodus puntazzo in Tunisia

SIMPLE SUMMARY: The mature spermatozoon of Peracreadium characis, an intestinal parasite of the sheephead bream, is described by means of transmission electron microscopy (TEM). It shares the common ultrastructural features described in the majority of digeneans (two 9+‘1’ axonemes, mitochondria, nucleus and parallel cortical microtubules). The absence of external ornamentation of the plasma membrane and spine-like bodies distinguishes the spermatozoon of P. characis from the spermatozoa of the remaining studied opecoelids. ABSTRACT: The spermatozoon ultrastructure of Peracreadium characis (Stossich, 1886) (Digenea: Opecoelidae), an intestinal parasite of the sheephead bream Diplodus puntazzo (Walbaum, 1792) (Sparidae), is described by means of transmission electron microscopy (TEM). The mature spermatozoon possesses two axonemes of the 9+‘1’ trepaxonematan pattern, an anterior electron-dense material, two mitochondria, a nucleus and parallel cortical microtubules distributed in two bundles. The absence of external ornamentation of the plasma membrane and spine-like bodies are the noteworthy characters that distinguish the spermatozoon of P. characis from those of most opecoelids. In fact, only Helicometra fasciata lacks external ornamentation in the spermatozoon. A comparative study with the remaining opecoelids described so far reveals similarities in the ultrastructural organization of their sperm cells. In addition, the current data on sperm ultrastructure in species of the recognized opecoelid subfamilies are compared, namely the Hamacreadiinae, Helicometrinae, Opecoelinae, Opistholebetinae and Plagioporinae.