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Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World

Aeromonas veronii is a Gram-negative bacterial species that causes disease in fish and is nowadays increasingly recurrent in enteric infections of humans. This study was performed to characterize newly sequenced isolates by comparing them with complete genomes deposited at the NCBI (National Center...

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Autores principales: Maia, José Cleves da Silva, Silva, Gabriel Amorim de Albuquerque, Cunha, Letícia Stheffany de Barros, Gouveia, Gisele Veneroni, Góes-Neto, Aristóteles, Brenig, Bertram, Araújo, Fabrício Almeida, Aburjaile, Flávia, Ramos, Rommel Thiago Jucá, Soares, Siomar Castro, Azevedo, Vasco Ariston de Carvalho, da Costa, Mateus Matiuzzi, Gouveia, João José de Simoni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295418/
https://www.ncbi.nlm.nih.gov/pubmed/37370358
http://dx.doi.org/10.3390/antibiotics12061039
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author Maia, José Cleves da Silva
Silva, Gabriel Amorim de Albuquerque
Cunha, Letícia Stheffany de Barros
Gouveia, Gisele Veneroni
Góes-Neto, Aristóteles
Brenig, Bertram
Araújo, Fabrício Almeida
Aburjaile, Flávia
Ramos, Rommel Thiago Jucá
Soares, Siomar Castro
Azevedo, Vasco Ariston de Carvalho
da Costa, Mateus Matiuzzi
Gouveia, João José de Simoni
author_facet Maia, José Cleves da Silva
Silva, Gabriel Amorim de Albuquerque
Cunha, Letícia Stheffany de Barros
Gouveia, Gisele Veneroni
Góes-Neto, Aristóteles
Brenig, Bertram
Araújo, Fabrício Almeida
Aburjaile, Flávia
Ramos, Rommel Thiago Jucá
Soares, Siomar Castro
Azevedo, Vasco Ariston de Carvalho
da Costa, Mateus Matiuzzi
Gouveia, João José de Simoni
author_sort Maia, José Cleves da Silva
collection PubMed
description Aeromonas veronii is a Gram-negative bacterial species that causes disease in fish and is nowadays increasingly recurrent in enteric infections of humans. This study was performed to characterize newly sequenced isolates by comparing them with complete genomes deposited at the NCBI (National Center for Biotechnology Information). Nine isolates from fish, environments, and humans from the São Francisco Valley (Petrolina, Pernambuco, Brazil) were sequenced and compared with complete genomes available in public databases to gain insight into taxonomic assignment and to better understand virulence and resistance profiles of this species within the One Health context. One local genome and four NCBI genomes were misidentified as A. veronii. A total of 239 virulence genes were identified in the local genomes, with most encoding adhesion, motility, and secretion systems. In total, 60 genes involved with resistance to 22 classes of antibiotics were identified in the genomes, including mcr-7 and cphA. The results suggest that the use of methods such as ANI is essential to avoid misclassification of the genomes. The virulence content of A. veronii from local isolates is similar to those complete genomes deposited at the NCBI. Genes encoding colistin resistance are widespread in the species, requiring greater attention for surveillance systems.
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spelling pubmed-102954182023-06-28 Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World Maia, José Cleves da Silva Silva, Gabriel Amorim de Albuquerque Cunha, Letícia Stheffany de Barros Gouveia, Gisele Veneroni Góes-Neto, Aristóteles Brenig, Bertram Araújo, Fabrício Almeida Aburjaile, Flávia Ramos, Rommel Thiago Jucá Soares, Siomar Castro Azevedo, Vasco Ariston de Carvalho da Costa, Mateus Matiuzzi Gouveia, João José de Simoni Antibiotics (Basel) Article Aeromonas veronii is a Gram-negative bacterial species that causes disease in fish and is nowadays increasingly recurrent in enteric infections of humans. This study was performed to characterize newly sequenced isolates by comparing them with complete genomes deposited at the NCBI (National Center for Biotechnology Information). Nine isolates from fish, environments, and humans from the São Francisco Valley (Petrolina, Pernambuco, Brazil) were sequenced and compared with complete genomes available in public databases to gain insight into taxonomic assignment and to better understand virulence and resistance profiles of this species within the One Health context. One local genome and four NCBI genomes were misidentified as A. veronii. A total of 239 virulence genes were identified in the local genomes, with most encoding adhesion, motility, and secretion systems. In total, 60 genes involved with resistance to 22 classes of antibiotics were identified in the genomes, including mcr-7 and cphA. The results suggest that the use of methods such as ANI is essential to avoid misclassification of the genomes. The virulence content of A. veronii from local isolates is similar to those complete genomes deposited at the NCBI. Genes encoding colistin resistance are widespread in the species, requiring greater attention for surveillance systems. MDPI 2023-06-11 /pmc/articles/PMC10295418/ /pubmed/37370358 http://dx.doi.org/10.3390/antibiotics12061039 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Maia, José Cleves da Silva
Silva, Gabriel Amorim de Albuquerque
Cunha, Letícia Stheffany de Barros
Gouveia, Gisele Veneroni
Góes-Neto, Aristóteles
Brenig, Bertram
Araújo, Fabrício Almeida
Aburjaile, Flávia
Ramos, Rommel Thiago Jucá
Soares, Siomar Castro
Azevedo, Vasco Ariston de Carvalho
da Costa, Mateus Matiuzzi
Gouveia, João José de Simoni
Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World
title Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World
title_full Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World
title_fullStr Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World
title_full_unstemmed Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World
title_short Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World
title_sort genomic characterization of aeromonas veronii provides insights into taxonomic assignment and reveals widespread virulence and resistance genes throughout the world
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295418/
https://www.ncbi.nlm.nih.gov/pubmed/37370358
http://dx.doi.org/10.3390/antibiotics12061039
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