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Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World
Aeromonas veronii is a Gram-negative bacterial species that causes disease in fish and is nowadays increasingly recurrent in enteric infections of humans. This study was performed to characterize newly sequenced isolates by comparing them with complete genomes deposited at the NCBI (National Center...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295418/ https://www.ncbi.nlm.nih.gov/pubmed/37370358 http://dx.doi.org/10.3390/antibiotics12061039 |
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author | Maia, José Cleves da Silva Silva, Gabriel Amorim de Albuquerque Cunha, Letícia Stheffany de Barros Gouveia, Gisele Veneroni Góes-Neto, Aristóteles Brenig, Bertram Araújo, Fabrício Almeida Aburjaile, Flávia Ramos, Rommel Thiago Jucá Soares, Siomar Castro Azevedo, Vasco Ariston de Carvalho da Costa, Mateus Matiuzzi Gouveia, João José de Simoni |
author_facet | Maia, José Cleves da Silva Silva, Gabriel Amorim de Albuquerque Cunha, Letícia Stheffany de Barros Gouveia, Gisele Veneroni Góes-Neto, Aristóteles Brenig, Bertram Araújo, Fabrício Almeida Aburjaile, Flávia Ramos, Rommel Thiago Jucá Soares, Siomar Castro Azevedo, Vasco Ariston de Carvalho da Costa, Mateus Matiuzzi Gouveia, João José de Simoni |
author_sort | Maia, José Cleves da Silva |
collection | PubMed |
description | Aeromonas veronii is a Gram-negative bacterial species that causes disease in fish and is nowadays increasingly recurrent in enteric infections of humans. This study was performed to characterize newly sequenced isolates by comparing them with complete genomes deposited at the NCBI (National Center for Biotechnology Information). Nine isolates from fish, environments, and humans from the São Francisco Valley (Petrolina, Pernambuco, Brazil) were sequenced and compared with complete genomes available in public databases to gain insight into taxonomic assignment and to better understand virulence and resistance profiles of this species within the One Health context. One local genome and four NCBI genomes were misidentified as A. veronii. A total of 239 virulence genes were identified in the local genomes, with most encoding adhesion, motility, and secretion systems. In total, 60 genes involved with resistance to 22 classes of antibiotics were identified in the genomes, including mcr-7 and cphA. The results suggest that the use of methods such as ANI is essential to avoid misclassification of the genomes. The virulence content of A. veronii from local isolates is similar to those complete genomes deposited at the NCBI. Genes encoding colistin resistance are widespread in the species, requiring greater attention for surveillance systems. |
format | Online Article Text |
id | pubmed-10295418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102954182023-06-28 Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World Maia, José Cleves da Silva Silva, Gabriel Amorim de Albuquerque Cunha, Letícia Stheffany de Barros Gouveia, Gisele Veneroni Góes-Neto, Aristóteles Brenig, Bertram Araújo, Fabrício Almeida Aburjaile, Flávia Ramos, Rommel Thiago Jucá Soares, Siomar Castro Azevedo, Vasco Ariston de Carvalho da Costa, Mateus Matiuzzi Gouveia, João José de Simoni Antibiotics (Basel) Article Aeromonas veronii is a Gram-negative bacterial species that causes disease in fish and is nowadays increasingly recurrent in enteric infections of humans. This study was performed to characterize newly sequenced isolates by comparing them with complete genomes deposited at the NCBI (National Center for Biotechnology Information). Nine isolates from fish, environments, and humans from the São Francisco Valley (Petrolina, Pernambuco, Brazil) were sequenced and compared with complete genomes available in public databases to gain insight into taxonomic assignment and to better understand virulence and resistance profiles of this species within the One Health context. One local genome and four NCBI genomes were misidentified as A. veronii. A total of 239 virulence genes were identified in the local genomes, with most encoding adhesion, motility, and secretion systems. In total, 60 genes involved with resistance to 22 classes of antibiotics were identified in the genomes, including mcr-7 and cphA. The results suggest that the use of methods such as ANI is essential to avoid misclassification of the genomes. The virulence content of A. veronii from local isolates is similar to those complete genomes deposited at the NCBI. Genes encoding colistin resistance are widespread in the species, requiring greater attention for surveillance systems. MDPI 2023-06-11 /pmc/articles/PMC10295418/ /pubmed/37370358 http://dx.doi.org/10.3390/antibiotics12061039 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Maia, José Cleves da Silva Silva, Gabriel Amorim de Albuquerque Cunha, Letícia Stheffany de Barros Gouveia, Gisele Veneroni Góes-Neto, Aristóteles Brenig, Bertram Araújo, Fabrício Almeida Aburjaile, Flávia Ramos, Rommel Thiago Jucá Soares, Siomar Castro Azevedo, Vasco Ariston de Carvalho da Costa, Mateus Matiuzzi Gouveia, João José de Simoni Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World |
title | Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World |
title_full | Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World |
title_fullStr | Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World |
title_full_unstemmed | Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World |
title_short | Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World |
title_sort | genomic characterization of aeromonas veronii provides insights into taxonomic assignment and reveals widespread virulence and resistance genes throughout the world |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295418/ https://www.ncbi.nlm.nih.gov/pubmed/37370358 http://dx.doi.org/10.3390/antibiotics12061039 |
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