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In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis
SIMPLE SUMMARY: Alphaherpesviruses cause genital lesions in both animals and humans. Ozone (O(3)) has a strong virucidal action on enveloped and naked viruses. The aim of this study was to test the in vitro virucidal and antiviral activity of an ozone/oxygen (O(3)/O(2)) gaseous mixture against capri...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295554/ https://www.ncbi.nlm.nih.gov/pubmed/37370430 http://dx.doi.org/10.3390/ani13121920 |
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author | Lillo, Edoardo Pellegrini, Francesco Rizzo, Annalisa Lanave, Gianvito Zizzadoro, Claudia Cicirelli, Vincenzo Catella, Cristiana Losurdo, Michele Martella, Vito Tempesta, Maria Camero, Michele |
author_facet | Lillo, Edoardo Pellegrini, Francesco Rizzo, Annalisa Lanave, Gianvito Zizzadoro, Claudia Cicirelli, Vincenzo Catella, Cristiana Losurdo, Michele Martella, Vito Tempesta, Maria Camero, Michele |
author_sort | Lillo, Edoardo |
collection | PubMed |
description | SIMPLE SUMMARY: Alphaherpesviruses cause genital lesions in both animals and humans. Ozone (O(3)) has a strong virucidal action on enveloped and naked viruses. The aim of this study was to test the in vitro virucidal and antiviral activity of an ozone/oxygen (O(3)/O(2)) gaseous mixture against caprine herpesvirus type 1 (CpHV-1). To test the virucidal activity, the virus was exposed to different concentrations (20 and 50 μg/mL) of the gaseous mixture at different time points, and a decrease in the viral titer by up to 2.0 log10 TCID(50)/50 μL was observed. To test the antiviral activity, the virus was exposed to different non-cytotoxic concentrations of the gaseous mixture. When MDBK cell monolayers were treated with the gas mixture after infection with CpHV-1 at a concentration of 50 μg/mL, significant antiviral activity was observed with a decrease in viral titer of 2.0 log10 TCID(50)/50 μL. These findings aid future studies aimed at assessing if topical treatment of genital herpes lesions in vivo with O(3)/O(2) gaseous mixture could be a valid and safe therapeutic option in an animal model, with possible translational applications in the therapy of human herpes simplex virus type 2 (HSV-2), which shares several biological similarities with CpHV-1. ABSTRACT: Alphaherpesviruses cause genital lesions and reproductive failure in both humans and animals. Their control is mainly based on prevention using hygienic prophylactic measures due to the absence of vaccines and limitations of antiviral drug therapy. Ozone is an oxidating gas showing a strong microbicidal activity on bacteria, fungi, viruses, and protozoa. The present study assessed the in vitro virucidal and antiviral activity of ozone against caprine herpesvirus type 1 (CpHV-1). The virucidal activity of a gaseous mixture containing O(3) at 20 and 50 μg/mL was assessed against the virus at different contact times (30 s, 60 s, 90 s, 120 s, 180 s, and 300 s). Antiviral activity of a gaseous mixture containing O(3) at 20 and 50 μg/mL was evaluated against the virus after 30 s and 60 s. Ozone displayed significant virucidal activity when used at all the tested concentrations whilst significant antiviral activity was observed using ozone at 50 μg/mL. The gaseous mixture, tested in the present study, showed virucidal and antiviral activity against CpHV-1 in a dose- and time contact-dependent fashion. Ozone therapy could be evaluated in vivo for the treatment of CpHV-1-induced genital lesions in goats using topical applications. |
format | Online Article Text |
id | pubmed-10295554 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102955542023-06-28 In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis Lillo, Edoardo Pellegrini, Francesco Rizzo, Annalisa Lanave, Gianvito Zizzadoro, Claudia Cicirelli, Vincenzo Catella, Cristiana Losurdo, Michele Martella, Vito Tempesta, Maria Camero, Michele Animals (Basel) Article SIMPLE SUMMARY: Alphaherpesviruses cause genital lesions in both animals and humans. Ozone (O(3)) has a strong virucidal action on enveloped and naked viruses. The aim of this study was to test the in vitro virucidal and antiviral activity of an ozone/oxygen (O(3)/O(2)) gaseous mixture against caprine herpesvirus type 1 (CpHV-1). To test the virucidal activity, the virus was exposed to different concentrations (20 and 50 μg/mL) of the gaseous mixture at different time points, and a decrease in the viral titer by up to 2.0 log10 TCID(50)/50 μL was observed. To test the antiviral activity, the virus was exposed to different non-cytotoxic concentrations of the gaseous mixture. When MDBK cell monolayers were treated with the gas mixture after infection with CpHV-1 at a concentration of 50 μg/mL, significant antiviral activity was observed with a decrease in viral titer of 2.0 log10 TCID(50)/50 μL. These findings aid future studies aimed at assessing if topical treatment of genital herpes lesions in vivo with O(3)/O(2) gaseous mixture could be a valid and safe therapeutic option in an animal model, with possible translational applications in the therapy of human herpes simplex virus type 2 (HSV-2), which shares several biological similarities with CpHV-1. ABSTRACT: Alphaherpesviruses cause genital lesions and reproductive failure in both humans and animals. Their control is mainly based on prevention using hygienic prophylactic measures due to the absence of vaccines and limitations of antiviral drug therapy. Ozone is an oxidating gas showing a strong microbicidal activity on bacteria, fungi, viruses, and protozoa. The present study assessed the in vitro virucidal and antiviral activity of ozone against caprine herpesvirus type 1 (CpHV-1). The virucidal activity of a gaseous mixture containing O(3) at 20 and 50 μg/mL was assessed against the virus at different contact times (30 s, 60 s, 90 s, 120 s, 180 s, and 300 s). Antiviral activity of a gaseous mixture containing O(3) at 20 and 50 μg/mL was evaluated against the virus after 30 s and 60 s. Ozone displayed significant virucidal activity when used at all the tested concentrations whilst significant antiviral activity was observed using ozone at 50 μg/mL. The gaseous mixture, tested in the present study, showed virucidal and antiviral activity against CpHV-1 in a dose- and time contact-dependent fashion. Ozone therapy could be evaluated in vivo for the treatment of CpHV-1-induced genital lesions in goats using topical applications. MDPI 2023-06-08 /pmc/articles/PMC10295554/ /pubmed/37370430 http://dx.doi.org/10.3390/ani13121920 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lillo, Edoardo Pellegrini, Francesco Rizzo, Annalisa Lanave, Gianvito Zizzadoro, Claudia Cicirelli, Vincenzo Catella, Cristiana Losurdo, Michele Martella, Vito Tempesta, Maria Camero, Michele In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis |
title | In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis |
title_full | In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis |
title_fullStr | In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis |
title_full_unstemmed | In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis |
title_short | In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis |
title_sort | in vitro activity of ozone/oxygen gaseous mixture against a caprine herpesvirus type 1 strain isolated from a goat with vaginitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295554/ https://www.ncbi.nlm.nih.gov/pubmed/37370430 http://dx.doi.org/10.3390/ani13121920 |
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