Cargando…

The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage

Traumatic brain injury (TBI) affects millions of people annually; however, our knowledge of the diffuse pathologies associated with TBI is limited. As diffuse pathologies, including axonal injury and neuroinflammatory changes, are difficult to visualize in the clinical population, animal models are...

Descripción completa

Detalles Bibliográficos
Autores principales: Pavlichenko, Mark, Lafrenaye, Audrey D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295711/
https://www.ncbi.nlm.nih.gov/pubmed/37371777
http://dx.doi.org/10.3390/biomedicines11061682
_version_ 1785063486169022464
author Pavlichenko, Mark
Lafrenaye, Audrey D.
author_facet Pavlichenko, Mark
Lafrenaye, Audrey D.
author_sort Pavlichenko, Mark
collection PubMed
description Traumatic brain injury (TBI) affects millions of people annually; however, our knowledge of the diffuse pathologies associated with TBI is limited. As diffuse pathologies, including axonal injury and neuroinflammatory changes, are difficult to visualize in the clinical population, animal models are used. In the current study, we used the central fluid percussion injury (CFPI) model in a micro pig to study the potential scalability of these diffuse pathologies in a gyrencephalic brain of a species with inflammatory systems very similar to humans. We found that both axonal injury and microglia activation within the thalamus and corpus callosum are positively correlated with the weight-normalized pressure pulse, while subtle changes in blood gas and mean arterial blood pressure are not. We also found that the majority of tissue generated up to 10 years previously is viable for immunofluorescent labeling after long-term refrigeration storage. This study indicates that a micro pig CFPI model could allow for specific investigations of various degrees of diffuse pathological burdens following TBI.
format Online
Article
Text
id pubmed-10295711
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102957112023-06-28 The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage Pavlichenko, Mark Lafrenaye, Audrey D. Biomedicines Article Traumatic brain injury (TBI) affects millions of people annually; however, our knowledge of the diffuse pathologies associated with TBI is limited. As diffuse pathologies, including axonal injury and neuroinflammatory changes, are difficult to visualize in the clinical population, animal models are used. In the current study, we used the central fluid percussion injury (CFPI) model in a micro pig to study the potential scalability of these diffuse pathologies in a gyrencephalic brain of a species with inflammatory systems very similar to humans. We found that both axonal injury and microglia activation within the thalamus and corpus callosum are positively correlated with the weight-normalized pressure pulse, while subtle changes in blood gas and mean arterial blood pressure are not. We also found that the majority of tissue generated up to 10 years previously is viable for immunofluorescent labeling after long-term refrigeration storage. This study indicates that a micro pig CFPI model could allow for specific investigations of various degrees of diffuse pathological burdens following TBI. MDPI 2023-06-10 /pmc/articles/PMC10295711/ /pubmed/37371777 http://dx.doi.org/10.3390/biomedicines11061682 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pavlichenko, Mark
Lafrenaye, Audrey D.
The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage
title The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage
title_full The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage
title_fullStr The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage
title_full_unstemmed The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage
title_short The Central Fluid Percussion Brain Injury in a Gyrencephalic Pig Brain: Scalable Diffuse Injury and Tissue Viability for Glial Cell Immunolabeling following Long-Term Refrigerated Storage
title_sort central fluid percussion brain injury in a gyrencephalic pig brain: scalable diffuse injury and tissue viability for glial cell immunolabeling following long-term refrigerated storage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295711/
https://www.ncbi.nlm.nih.gov/pubmed/37371777
http://dx.doi.org/10.3390/biomedicines11061682
work_keys_str_mv AT pavlichenkomark thecentralfluidpercussionbraininjuryinagyrencephalicpigbrainscalablediffuseinjuryandtissueviabilityforglialcellimmunolabelingfollowinglongtermrefrigeratedstorage
AT lafrenayeaudreyd thecentralfluidpercussionbraininjuryinagyrencephalicpigbrainscalablediffuseinjuryandtissueviabilityforglialcellimmunolabelingfollowinglongtermrefrigeratedstorage
AT pavlichenkomark centralfluidpercussionbraininjuryinagyrencephalicpigbrainscalablediffuseinjuryandtissueviabilityforglialcellimmunolabelingfollowinglongtermrefrigeratedstorage
AT lafrenayeaudreyd centralfluidpercussionbraininjuryinagyrencephalicpigbrainscalablediffuseinjuryandtissueviabilityforglialcellimmunolabelingfollowinglongtermrefrigeratedstorage