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Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model

Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the in vitro and in vivo anticancer activity of hemocyanins isolated from Helix aspersa, Helix lucorum,...

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Autores principales: Georgieva, Ani, Todorova, Katerina, Iliev, Ivan, Dilcheva, Valeria, Vladov, Ivelin, Petkova, Svetlozara, Dolashki, Aleksandar, Velkova, Lyudmila, Dolashka, Pavlina, Toshkova, Reneta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295749/
https://www.ncbi.nlm.nih.gov/pubmed/37371641
http://dx.doi.org/10.3390/biomedicines11061545
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author Georgieva, Ani
Todorova, Katerina
Iliev, Ivan
Dilcheva, Valeria
Vladov, Ivelin
Petkova, Svetlozara
Dolashki, Aleksandar
Velkova, Lyudmila
Dolashka, Pavlina
Toshkova, Reneta
author_facet Georgieva, Ani
Todorova, Katerina
Iliev, Ivan
Dilcheva, Valeria
Vladov, Ivelin
Petkova, Svetlozara
Dolashki, Aleksandar
Velkova, Lyudmila
Dolashka, Pavlina
Toshkova, Reneta
author_sort Georgieva, Ani
collection PubMed
description Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the in vitro and in vivo anticancer activity of hemocyanins isolated from Helix aspersa, Helix lucorum, and Rapana venosa in the Graffi myeloid tumor model. The in vitro antitumor activity of the hemocyanins was determined by a MTT test and cytomorphological analysis by fluorescent and transmission electron microscopy. The in vivo effects of the hemocyanins were examined in hamsters transplanted with Graffi tumor. The serum antibody titers against the tested hemocyanins and tumor antigen were determined by ELISA. Histopathological assessment of the morphological features related to antitumor effect, immune system response, and toxicity in some internal organs was performed. The results of in vitro studies indicated that the tested hemocyanins induced significant antiproliferative and apoptogenic effects. The in vivo investigations demonstrated a protective antitumor effect, expressed in reduced transplantability, suppression of tumor growth and metastasis, reduced mortality, prolonged survival time, and absence of toxic side effects. The present study indicated that the antitumor activity of the studied hemocyanins was due to both immune stimulation and direct effects on the tumor cells, and they displayed their potential as therapeutic agents against hematological malignances.
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spelling pubmed-102957492023-06-28 Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model Georgieva, Ani Todorova, Katerina Iliev, Ivan Dilcheva, Valeria Vladov, Ivelin Petkova, Svetlozara Dolashki, Aleksandar Velkova, Lyudmila Dolashka, Pavlina Toshkova, Reneta Biomedicines Article Hemocyanins are oxygen-transporting glycoproteins in the hemolymph of some invertebrate species that attracted scientific interest as potential anticancer agents. The present study aims to assess the in vitro and in vivo anticancer activity of hemocyanins isolated from Helix aspersa, Helix lucorum, and Rapana venosa in the Graffi myeloid tumor model. The in vitro antitumor activity of the hemocyanins was determined by a MTT test and cytomorphological analysis by fluorescent and transmission electron microscopy. The in vivo effects of the hemocyanins were examined in hamsters transplanted with Graffi tumor. The serum antibody titers against the tested hemocyanins and tumor antigen were determined by ELISA. Histopathological assessment of the morphological features related to antitumor effect, immune system response, and toxicity in some internal organs was performed. The results of in vitro studies indicated that the tested hemocyanins induced significant antiproliferative and apoptogenic effects. The in vivo investigations demonstrated a protective antitumor effect, expressed in reduced transplantability, suppression of tumor growth and metastasis, reduced mortality, prolonged survival time, and absence of toxic side effects. The present study indicated that the antitumor activity of the studied hemocyanins was due to both immune stimulation and direct effects on the tumor cells, and they displayed their potential as therapeutic agents against hematological malignances. MDPI 2023-05-26 /pmc/articles/PMC10295749/ /pubmed/37371641 http://dx.doi.org/10.3390/biomedicines11061545 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Georgieva, Ani
Todorova, Katerina
Iliev, Ivan
Dilcheva, Valeria
Vladov, Ivelin
Petkova, Svetlozara
Dolashki, Aleksandar
Velkova, Lyudmila
Dolashka, Pavlina
Toshkova, Reneta
Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model
title Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model
title_full Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model
title_fullStr Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model
title_full_unstemmed Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model
title_short Assessment of the In Vitro and In Vivo Antitumor Activity of Hemocyanins from Helix aspersa, Helix lucorum, and Rapana venosa in a Graffi Myeloid Tumor Model
title_sort assessment of the in vitro and in vivo antitumor activity of hemocyanins from helix aspersa, helix lucorum, and rapana venosa in a graffi myeloid tumor model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295749/
https://www.ncbi.nlm.nih.gov/pubmed/37371641
http://dx.doi.org/10.3390/biomedicines11061545
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