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35 Years of TFAM Research: Old Protein, New Puzzles
SIMPLE SUMMARY: Transcription Factor A Mitochondrial (TFAM) was first purified 35 years ago as an activator of mitochondrial transcription. Because of its critical contributions to mitochondrial DNA transcription and replication, this protein was viewed as a master regulator of mitochondrial biogene...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295803/ https://www.ncbi.nlm.nih.gov/pubmed/37372108 http://dx.doi.org/10.3390/biology12060823 |
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author | Kozhukhar, Natalya Alexeyev, Mikhail F. |
author_facet | Kozhukhar, Natalya Alexeyev, Mikhail F. |
author_sort | Kozhukhar, Natalya |
collection | PubMed |
description | SIMPLE SUMMARY: Transcription Factor A Mitochondrial (TFAM) was first purified 35 years ago as an activator of mitochondrial transcription. Because of its critical contributions to mitochondrial DNA transcription and replication, this protein was viewed as a master regulator of mitochondrial biogenesis. However, now it is clear that TFAM’s cellular role is more nuanced than previously thought. In this review, we attempted to compile and assess various, at times contradictory, lines of evidence supporting this protein’s diverse roles. It is carried out in the belief that the resolution of the existing contradictions is a prerequisite for further advancements in the field. ABSTRACT: Transcription Factor A Mitochondrial (TFAM), through its contributions to mtDNA maintenance and expression, is essential for cellular bioenergetics and, therefore, for the very survival of cells. Thirty-five years of research on TFAM structure and function generated a considerable body of experimental evidence, some of which remains to be fully reconciled. Recent advancements allowed an unprecedented glimpse into the structure of TFAM complexed with promoter DNA and TFAM within the open promoter complexes. These novel insights, however, raise new questions about the function of this remarkable protein. In our review, we compile the available literature on TFAM structure and function and provide some critical analysis of the available data. |
format | Online Article Text |
id | pubmed-10295803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102958032023-06-28 35 Years of TFAM Research: Old Protein, New Puzzles Kozhukhar, Natalya Alexeyev, Mikhail F. Biology (Basel) Review SIMPLE SUMMARY: Transcription Factor A Mitochondrial (TFAM) was first purified 35 years ago as an activator of mitochondrial transcription. Because of its critical contributions to mitochondrial DNA transcription and replication, this protein was viewed as a master regulator of mitochondrial biogenesis. However, now it is clear that TFAM’s cellular role is more nuanced than previously thought. In this review, we attempted to compile and assess various, at times contradictory, lines of evidence supporting this protein’s diverse roles. It is carried out in the belief that the resolution of the existing contradictions is a prerequisite for further advancements in the field. ABSTRACT: Transcription Factor A Mitochondrial (TFAM), through its contributions to mtDNA maintenance and expression, is essential for cellular bioenergetics and, therefore, for the very survival of cells. Thirty-five years of research on TFAM structure and function generated a considerable body of experimental evidence, some of which remains to be fully reconciled. Recent advancements allowed an unprecedented glimpse into the structure of TFAM complexed with promoter DNA and TFAM within the open promoter complexes. These novel insights, however, raise new questions about the function of this remarkable protein. In our review, we compile the available literature on TFAM structure and function and provide some critical analysis of the available data. MDPI 2023-06-06 /pmc/articles/PMC10295803/ /pubmed/37372108 http://dx.doi.org/10.3390/biology12060823 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Kozhukhar, Natalya Alexeyev, Mikhail F. 35 Years of TFAM Research: Old Protein, New Puzzles |
title | 35 Years of TFAM Research: Old Protein, New Puzzles |
title_full | 35 Years of TFAM Research: Old Protein, New Puzzles |
title_fullStr | 35 Years of TFAM Research: Old Protein, New Puzzles |
title_full_unstemmed | 35 Years of TFAM Research: Old Protein, New Puzzles |
title_short | 35 Years of TFAM Research: Old Protein, New Puzzles |
title_sort | 35 years of tfam research: old protein, new puzzles |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295803/ https://www.ncbi.nlm.nih.gov/pubmed/37372108 http://dx.doi.org/10.3390/biology12060823 |
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