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Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement

Currently, platelet-rich plasma (PRP) is an attractive additive for bone repair materials. PRP could enhance the osteoconductive and osteoinductive of bone cement, as well as modulate the degradation rate of calcium sulfate hemihydrate (CSH). The focus of this study was to investigate the effect of...

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Autores principales: Liu, Jingyu, Wang, Yifan, Liang, Yanqin, Zhu, Shengli, Jiang, Hui, Wu, Shuilin, Ge, Xiang, Li, Zhaoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295830/
https://www.ncbi.nlm.nih.gov/pubmed/37366857
http://dx.doi.org/10.3390/biomimetics8020262
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author Liu, Jingyu
Wang, Yifan
Liang, Yanqin
Zhu, Shengli
Jiang, Hui
Wu, Shuilin
Ge, Xiang
Li, Zhaoyang
author_facet Liu, Jingyu
Wang, Yifan
Liang, Yanqin
Zhu, Shengli
Jiang, Hui
Wu, Shuilin
Ge, Xiang
Li, Zhaoyang
author_sort Liu, Jingyu
collection PubMed
description Currently, platelet-rich plasma (PRP) is an attractive additive for bone repair materials. PRP could enhance the osteoconductive and osteoinductive of bone cement, as well as modulate the degradation rate of calcium sulfate hemihydrate (CSH). The focus of this study was to investigate the effect of different PRP ratios (P1: 20 vol%, P2: 40 vol%, and P3: 60 vol%) on the chemical properties and biological activity of bone cement. The injectability and compressive strength of the experimental group were significantly higher than those of the control. On the other hand, the addition of PRP decreased the crystal size of CSH and prolonged the degradation time. More importantly, the cell proliferation of L929 and MC3T3-E1 cells was promoted. Furthermore, qRT-PCR, alizarin red staining, and western blot analyses showed that the expressions of osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) genes and β-catenin protein were up-regulated, and mineralization of extracellular matrix was enhanced. Overall, this study provided insight into how to improve the biological activity of bone cement through PRP incorporation.
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spelling pubmed-102958302023-06-28 Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement Liu, Jingyu Wang, Yifan Liang, Yanqin Zhu, Shengli Jiang, Hui Wu, Shuilin Ge, Xiang Li, Zhaoyang Biomimetics (Basel) Article Currently, platelet-rich plasma (PRP) is an attractive additive for bone repair materials. PRP could enhance the osteoconductive and osteoinductive of bone cement, as well as modulate the degradation rate of calcium sulfate hemihydrate (CSH). The focus of this study was to investigate the effect of different PRP ratios (P1: 20 vol%, P2: 40 vol%, and P3: 60 vol%) on the chemical properties and biological activity of bone cement. The injectability and compressive strength of the experimental group were significantly higher than those of the control. On the other hand, the addition of PRP decreased the crystal size of CSH and prolonged the degradation time. More importantly, the cell proliferation of L929 and MC3T3-E1 cells was promoted. Furthermore, qRT-PCR, alizarin red staining, and western blot analyses showed that the expressions of osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) genes and β-catenin protein were up-regulated, and mineralization of extracellular matrix was enhanced. Overall, this study provided insight into how to improve the biological activity of bone cement through PRP incorporation. MDPI 2023-06-15 /pmc/articles/PMC10295830/ /pubmed/37366857 http://dx.doi.org/10.3390/biomimetics8020262 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Jingyu
Wang, Yifan
Liang, Yanqin
Zhu, Shengli
Jiang, Hui
Wu, Shuilin
Ge, Xiang
Li, Zhaoyang
Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement
title Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement
title_full Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement
title_fullStr Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement
title_full_unstemmed Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement
title_short Effect of Platelet-Rich Plasma Addition on the Chemical Properties and Biological Activity of Calcium Sulfate Hemihydrate Bone Cement
title_sort effect of platelet-rich plasma addition on the chemical properties and biological activity of calcium sulfate hemihydrate bone cement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295830/
https://www.ncbi.nlm.nih.gov/pubmed/37366857
http://dx.doi.org/10.3390/biomimetics8020262
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