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Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions
A newly developed analytical strategy was applied to profile the total serum N-glycome of 64 colorectal cancer (CRC) patients before and after surgical intervention. In this cohort, it was previously found that serum N-glycome alterations in CRC were associated with patient survival. Here, fluoresce...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295937/ https://www.ncbi.nlm.nih.gov/pubmed/37371476 http://dx.doi.org/10.3390/biom13060896 |
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author | Moran, Alan B. Elgood-Hunt, Georgia van der Burgt, Yuri E. M. Wuhrer, Manfred Mesker, Wilma E. Tollenaar, Rob A. E. M. Spencer, Daniel I. R. Lageveen-Kammeijer, Guinevere S. M. |
author_facet | Moran, Alan B. Elgood-Hunt, Georgia van der Burgt, Yuri E. M. Wuhrer, Manfred Mesker, Wilma E. Tollenaar, Rob A. E. M. Spencer, Daniel I. R. Lageveen-Kammeijer, Guinevere S. M. |
author_sort | Moran, Alan B. |
collection | PubMed |
description | A newly developed analytical strategy was applied to profile the total serum N-glycome of 64 colorectal cancer (CRC) patients before and after surgical intervention. In this cohort, it was previously found that serum N-glycome alterations in CRC were associated with patient survival. Here, fluorescent labeling of serum N-glycans was applied using procainamide and followed by sialic acid derivatization specific for α2,6- and α2,3-linkage types via ethyl esterification and amidation, respectively. This strategy allowed efficient separation of specific positional isomers on reversed-phase liquid chromatography–fluorescence detection–mass spectrometry (RPLC-FD-MS) and complemented the previous glycomics data based on matrix-assisted laser desorption/ionization (MALDI)-MS that did not include such separations. The results from comparing pre-operative CRC to post-operative samples were in agreement with studies that identified a decrease in di-antennary structures with core fucosylation and an increase in sialylated tri- and tetra-antennary N-glycans in CRC patient sera. Pre-operative abundances of N-glycans showed good performance for the classification of adenocarcinoma and led to the revisit of the previous MALDI-MS dataset with regard to histological and clinical data. This strategy has the potential to monitor patient profiles before, during, and after clinical events such as treatment, therapy, or surgery and should also be further explored. |
format | Online Article Text |
id | pubmed-10295937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102959372023-06-28 Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions Moran, Alan B. Elgood-Hunt, Georgia van der Burgt, Yuri E. M. Wuhrer, Manfred Mesker, Wilma E. Tollenaar, Rob A. E. M. Spencer, Daniel I. R. Lageveen-Kammeijer, Guinevere S. M. Biomolecules Article A newly developed analytical strategy was applied to profile the total serum N-glycome of 64 colorectal cancer (CRC) patients before and after surgical intervention. In this cohort, it was previously found that serum N-glycome alterations in CRC were associated with patient survival. Here, fluorescent labeling of serum N-glycans was applied using procainamide and followed by sialic acid derivatization specific for α2,6- and α2,3-linkage types via ethyl esterification and amidation, respectively. This strategy allowed efficient separation of specific positional isomers on reversed-phase liquid chromatography–fluorescence detection–mass spectrometry (RPLC-FD-MS) and complemented the previous glycomics data based on matrix-assisted laser desorption/ionization (MALDI)-MS that did not include such separations. The results from comparing pre-operative CRC to post-operative samples were in agreement with studies that identified a decrease in di-antennary structures with core fucosylation and an increase in sialylated tri- and tetra-antennary N-glycans in CRC patient sera. Pre-operative abundances of N-glycans showed good performance for the classification of adenocarcinoma and led to the revisit of the previous MALDI-MS dataset with regard to histological and clinical data. This strategy has the potential to monitor patient profiles before, during, and after clinical events such as treatment, therapy, or surgery and should also be further explored. MDPI 2023-05-27 /pmc/articles/PMC10295937/ /pubmed/37371476 http://dx.doi.org/10.3390/biom13060896 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moran, Alan B. Elgood-Hunt, Georgia van der Burgt, Yuri E. M. Wuhrer, Manfred Mesker, Wilma E. Tollenaar, Rob A. E. M. Spencer, Daniel I. R. Lageveen-Kammeijer, Guinevere S. M. Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions |
title | Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions |
title_full | Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions |
title_fullStr | Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions |
title_full_unstemmed | Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions |
title_short | Serum N-Glycosylation RPLC-FD-MS Assay to Assess Colorectal Cancer Surgical Interventions |
title_sort | serum n-glycosylation rplc-fd-ms assay to assess colorectal cancer surgical interventions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295937/ https://www.ncbi.nlm.nih.gov/pubmed/37371476 http://dx.doi.org/10.3390/biom13060896 |
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