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Dynamics of Urinary Extracellular DNA in Urosepsis

Extracellular DNA (ecDNA) is a promising candidate marker for the early diagnosis and monitoring of urinary tract infections (UTIs). The aim of our study is to describe the dynamics of ecDNA in the plasma and urine of patients with urosepsis as well as in a mouse model of UTI. Samples of blood and u...

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Autores principales: Mihaľová, Michaela, Šupčíková, Nadja, Kovalčíková, Alexandra Gaál, Breza, Ján, Tóthová, Ľubomíra, Celec, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295957/
https://www.ncbi.nlm.nih.gov/pubmed/37371588
http://dx.doi.org/10.3390/biom13061008
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author Mihaľová, Michaela
Šupčíková, Nadja
Kovalčíková, Alexandra Gaál
Breza, Ján
Tóthová, Ľubomíra
Celec, Peter
Breza, Ján
author_facet Mihaľová, Michaela
Šupčíková, Nadja
Kovalčíková, Alexandra Gaál
Breza, Ján
Tóthová, Ľubomíra
Celec, Peter
Breza, Ján
author_sort Mihaľová, Michaela
collection PubMed
description Extracellular DNA (ecDNA) is a promising candidate marker for the early diagnosis and monitoring of urinary tract infections (UTIs). The aim of our study is to describe the dynamics of ecDNA in the plasma and urine of patients with urosepsis as well as in a mouse model of UTI. Samples of blood and urine were collected from adult patients with UTIs and obstructive uropathy (n = 36) during the first 3 days at the hospital and during a follow-up. Bacterial burden and urinary ecDNA were evaluated in a mouse UTI model (n = 26) at baseline; 24, 48, and 72 h after UTI induction; and 7 days after UTI induction. The plasma ecDNA did not change during urosepsis, but the plasma DNase activity increased significantly at the follow-up. The urinary ecDNA decreased significantly during hospitalization and remained low until the follow-up (90% lower vs. admission). No change was seen in the urinary DNase activity. C-reactive protein (CRP) and procalcitonin are positively correlated with plasma and urinary ecDNA. A UTI caused sepsis in 23% of mice. The urinary ecDNA decreased by three-fold and remained low until day 7 post-infection. Urinary bacterial burden is correlated with urinary ecDNA. Urinary ecDNA is a potential non-invasive marker for monitoring the effects of treatment during urosepsis and is related to UTI progression in the experimental animal model.
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spelling pubmed-102959572023-06-28 Dynamics of Urinary Extracellular DNA in Urosepsis Mihaľová, Michaela Šupčíková, Nadja Kovalčíková, Alexandra Gaál Breza, Ján Tóthová, Ľubomíra Celec, Peter Breza, Ján Biomolecules Article Extracellular DNA (ecDNA) is a promising candidate marker for the early diagnosis and monitoring of urinary tract infections (UTIs). The aim of our study is to describe the dynamics of ecDNA in the plasma and urine of patients with urosepsis as well as in a mouse model of UTI. Samples of blood and urine were collected from adult patients with UTIs and obstructive uropathy (n = 36) during the first 3 days at the hospital and during a follow-up. Bacterial burden and urinary ecDNA were evaluated in a mouse UTI model (n = 26) at baseline; 24, 48, and 72 h after UTI induction; and 7 days after UTI induction. The plasma ecDNA did not change during urosepsis, but the plasma DNase activity increased significantly at the follow-up. The urinary ecDNA decreased significantly during hospitalization and remained low until the follow-up (90% lower vs. admission). No change was seen in the urinary DNase activity. C-reactive protein (CRP) and procalcitonin are positively correlated with plasma and urinary ecDNA. A UTI caused sepsis in 23% of mice. The urinary ecDNA decreased by three-fold and remained low until day 7 post-infection. Urinary bacterial burden is correlated with urinary ecDNA. Urinary ecDNA is a potential non-invasive marker for monitoring the effects of treatment during urosepsis and is related to UTI progression in the experimental animal model. MDPI 2023-06-17 /pmc/articles/PMC10295957/ /pubmed/37371588 http://dx.doi.org/10.3390/biom13061008 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mihaľová, Michaela
Šupčíková, Nadja
Kovalčíková, Alexandra Gaál
Breza, Ján
Tóthová, Ľubomíra
Celec, Peter
Breza, Ján
Dynamics of Urinary Extracellular DNA in Urosepsis
title Dynamics of Urinary Extracellular DNA in Urosepsis
title_full Dynamics of Urinary Extracellular DNA in Urosepsis
title_fullStr Dynamics of Urinary Extracellular DNA in Urosepsis
title_full_unstemmed Dynamics of Urinary Extracellular DNA in Urosepsis
title_short Dynamics of Urinary Extracellular DNA in Urosepsis
title_sort dynamics of urinary extracellular dna in urosepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295957/
https://www.ncbi.nlm.nih.gov/pubmed/37371588
http://dx.doi.org/10.3390/biom13061008
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