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Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaralde...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295976/ https://www.ncbi.nlm.nih.gov/pubmed/37366947 http://dx.doi.org/10.3390/bios13060582 |
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author | Navarro-Nateras, L. Diaz-Gonzalez, Jancarlo Aguas-Chantes, Diana Coria-Oriundo, Lucy L. Battaglini, Fernando Ventura-Gallegos, José Luis Zentella-Dehesa, Alejandro Oza, Goldie Arriaga, L. G. Casanova-Moreno, Jannu R. |
author_facet | Navarro-Nateras, L. Diaz-Gonzalez, Jancarlo Aguas-Chantes, Diana Coria-Oriundo, Lucy L. Battaglini, Fernando Ventura-Gallegos, José Luis Zentella-Dehesa, Alejandro Oza, Goldie Arriaga, L. G. Casanova-Moreno, Jannu R. |
author_sort | Navarro-Nateras, L. |
collection | PubMed |
description | The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H(2)O(2) diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM(−1) cm(−2). |
format | Online Article Text |
id | pubmed-10295976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102959762023-06-28 Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems Navarro-Nateras, L. Diaz-Gonzalez, Jancarlo Aguas-Chantes, Diana Coria-Oriundo, Lucy L. Battaglini, Fernando Ventura-Gallegos, José Luis Zentella-Dehesa, Alejandro Oza, Goldie Arriaga, L. G. Casanova-Moreno, Jannu R. Biosensors (Basel) Article The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H(2)O(2) diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM(−1) cm(−2). MDPI 2023-05-27 /pmc/articles/PMC10295976/ /pubmed/37366947 http://dx.doi.org/10.3390/bios13060582 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Navarro-Nateras, L. Diaz-Gonzalez, Jancarlo Aguas-Chantes, Diana Coria-Oriundo, Lucy L. Battaglini, Fernando Ventura-Gallegos, José Luis Zentella-Dehesa, Alejandro Oza, Goldie Arriaga, L. G. Casanova-Moreno, Jannu R. Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems |
title | Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems |
title_full | Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems |
title_fullStr | Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems |
title_full_unstemmed | Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems |
title_short | Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems |
title_sort | development of a redox-polymer-based electrochemical glucose biosensor suitable for integration in microfluidic 3d cell culture systems |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10295976/ https://www.ncbi.nlm.nih.gov/pubmed/37366947 http://dx.doi.org/10.3390/bios13060582 |
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