A Novel Fluorescent Probe for the Detection of Hydrogen Peroxide

Hydrogen peroxide (H(2)O(2)) is one of the important reactive oxygen species (ROS), which is closely related to many pathological and physiological processes in living organisms. Excessive H(2)O(2) can lead to cancer, diabetes, cardiovascular diseases, and other diseases, so it is necessary to detect H(2)O(2) in living cells. Since this work designed a novel fluorescent probe to detect the concentration of H(2)O(2), the H(2)O(2) reaction group arylboric acid was attached to the fluorescein 3-Acetyl-7-hydroxycoumarin as a specific recognition group for the selective detection of hydrogen peroxide. The experimental results show that the probe can effectively detect H(2)O(2) with high selectivity and measure cellular ROS levels. Therefore, this novel fluorescent probe provides a potential monitoring tool for a variety of diseases caused by H(2)O(2) excess.