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Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study

Microbiota composition might play a role in the pathophysiology and course of sepsis, and understanding its dynamics is of clinical interest. Invasive meningococcal disease (IMD) is an important cause of community-acquired serious infection, and there is no information regarding microbiota compositi...

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Autores principales: Bozan, Gurkan, Pérez-Brocal, Vicente, Aslan, Kaan, Kiral, Eylem, Sevketoglu, Esra, Uysal Yazici, Mutlu, Azapagasi, Ebru, Kendirli, Tanil, Emeksiz, Serhat, Dursun, Oguz, Yildizdas, Dincer, Anil, Ayse Berna, Akcay, Nihal, Kihtir, Hasan Serdar, Havan, Merve, Ulgen Tekerek, Nazan, Ekinci, Faruk, Kilic, Omer, Moya, Andres, Dinleyici, Ener Cagri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10296867/
https://www.ncbi.nlm.nih.gov/pubmed/37370879
http://dx.doi.org/10.3390/diagnostics13121984
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author Bozan, Gurkan
Pérez-Brocal, Vicente
Aslan, Kaan
Kiral, Eylem
Sevketoglu, Esra
Uysal Yazici, Mutlu
Azapagasi, Ebru
Kendirli, Tanil
Emeksiz, Serhat
Dursun, Oguz
Yildizdas, Dincer
Anil, Ayse Berna
Akcay, Nihal
Kihtir, Hasan Serdar
Havan, Merve
Ulgen Tekerek, Nazan
Ekinci, Faruk
Kilic, Omer
Moya, Andres
Dinleyici, Ener Cagri
author_facet Bozan, Gurkan
Pérez-Brocal, Vicente
Aslan, Kaan
Kiral, Eylem
Sevketoglu, Esra
Uysal Yazici, Mutlu
Azapagasi, Ebru
Kendirli, Tanil
Emeksiz, Serhat
Dursun, Oguz
Yildizdas, Dincer
Anil, Ayse Berna
Akcay, Nihal
Kihtir, Hasan Serdar
Havan, Merve
Ulgen Tekerek, Nazan
Ekinci, Faruk
Kilic, Omer
Moya, Andres
Dinleyici, Ener Cagri
author_sort Bozan, Gurkan
collection PubMed
description Microbiota composition might play a role in the pathophysiology and course of sepsis, and understanding its dynamics is of clinical interest. Invasive meningococcal disease (IMD) is an important cause of community-acquired serious infection, and there is no information regarding microbiota composition in children with meningococcemia. In this study, we aimed to evaluate the intestinal and nasopharyngeal microbiota composition of children with IMD. Materials and Methods: In this prospective, multi-center study, 10 children with meningococcemia and 10 age-matched healthy controls were included. Nasopharyngeal and fecal samples were obtained at admission to the intensive care unit and on the tenth day of their hospital stay. The V3 and V4 regions of the 16S rRNA gene were amplified following the 16S Metagenomic Sequencing Library Preparation. Results: Regarding the alpha diversity on the day of admission and on the tenth day at the PICU, the Shannon index was significantly lower in the IMD group compared to the control group (p = 0.002 at admission and p = 0.001, on the tenth day of PICU). A statistical difference in the stool samples was found between the IMD group at Day 0 vs. the controls in the results of the Bray–Curtis and Jaccard analyses (p = 0.005 and p = 0.001, respectively). There were differences in the intestinal microbiota composition between the children with IMD at admission and Day 10 and the healthy controls. Regarding the nasopharyngeal microbiota analysis, in the children with IMD at admission, at the genus level, Neisseria was significantly more abundant compared to the healthy children (p < 0.001). In the children with IMD at Day 10, genera Moraxella and Neisseria were decreased compared to the healthy children. In the children with IMD on Day 0, for paired samples, Moraxella, Neisseria, and Haemophilus were significantly more abundant compared to the children with IMD at Day 10. In the children with IMD at Day 10, the Moraxella and Neisseria genera were decreased, and 20 different genera were more abundant compared to Day 0. Conclusions: We first found alterations in the intestinal and nasopharyngeal microbiota composition in the children with IMD. The infection itself or the other care interventions also caused changes to the microbiota composition during the follow-up period. Understanding the interaction of microbiota with pathogens, e.g., N. meningitidis, could give us the opportunity to understand the disease’s dynamics.
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spelling pubmed-102968672023-06-28 Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study Bozan, Gurkan Pérez-Brocal, Vicente Aslan, Kaan Kiral, Eylem Sevketoglu, Esra Uysal Yazici, Mutlu Azapagasi, Ebru Kendirli, Tanil Emeksiz, Serhat Dursun, Oguz Yildizdas, Dincer Anil, Ayse Berna Akcay, Nihal Kihtir, Hasan Serdar Havan, Merve Ulgen Tekerek, Nazan Ekinci, Faruk Kilic, Omer Moya, Andres Dinleyici, Ener Cagri Diagnostics (Basel) Article Microbiota composition might play a role in the pathophysiology and course of sepsis, and understanding its dynamics is of clinical interest. Invasive meningococcal disease (IMD) is an important cause of community-acquired serious infection, and there is no information regarding microbiota composition in children with meningococcemia. In this study, we aimed to evaluate the intestinal and nasopharyngeal microbiota composition of children with IMD. Materials and Methods: In this prospective, multi-center study, 10 children with meningococcemia and 10 age-matched healthy controls were included. Nasopharyngeal and fecal samples were obtained at admission to the intensive care unit and on the tenth day of their hospital stay. The V3 and V4 regions of the 16S rRNA gene were amplified following the 16S Metagenomic Sequencing Library Preparation. Results: Regarding the alpha diversity on the day of admission and on the tenth day at the PICU, the Shannon index was significantly lower in the IMD group compared to the control group (p = 0.002 at admission and p = 0.001, on the tenth day of PICU). A statistical difference in the stool samples was found between the IMD group at Day 0 vs. the controls in the results of the Bray–Curtis and Jaccard analyses (p = 0.005 and p = 0.001, respectively). There were differences in the intestinal microbiota composition between the children with IMD at admission and Day 10 and the healthy controls. Regarding the nasopharyngeal microbiota analysis, in the children with IMD at admission, at the genus level, Neisseria was significantly more abundant compared to the healthy children (p < 0.001). In the children with IMD at Day 10, genera Moraxella and Neisseria were decreased compared to the healthy children. In the children with IMD on Day 0, for paired samples, Moraxella, Neisseria, and Haemophilus were significantly more abundant compared to the children with IMD at Day 10. In the children with IMD at Day 10, the Moraxella and Neisseria genera were decreased, and 20 different genera were more abundant compared to Day 0. Conclusions: We first found alterations in the intestinal and nasopharyngeal microbiota composition in the children with IMD. The infection itself or the other care interventions also caused changes to the microbiota composition during the follow-up period. Understanding the interaction of microbiota with pathogens, e.g., N. meningitidis, could give us the opportunity to understand the disease’s dynamics. MDPI 2023-06-06 /pmc/articles/PMC10296867/ /pubmed/37370879 http://dx.doi.org/10.3390/diagnostics13121984 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bozan, Gurkan
Pérez-Brocal, Vicente
Aslan, Kaan
Kiral, Eylem
Sevketoglu, Esra
Uysal Yazici, Mutlu
Azapagasi, Ebru
Kendirli, Tanil
Emeksiz, Serhat
Dursun, Oguz
Yildizdas, Dincer
Anil, Ayse Berna
Akcay, Nihal
Kihtir, Hasan Serdar
Havan, Merve
Ulgen Tekerek, Nazan
Ekinci, Faruk
Kilic, Omer
Moya, Andres
Dinleyici, Ener Cagri
Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study
title Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study
title_full Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study
title_fullStr Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study
title_full_unstemmed Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study
title_short Analysis of Intestinal and Nasopharyngeal Microbiota of Children with Meningococcemia in Pediatric Intensive Care Unit: INMACS-PICU Study
title_sort analysis of intestinal and nasopharyngeal microbiota of children with meningococcemia in pediatric intensive care unit: inmacs-picu study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10296867/
https://www.ncbi.nlm.nih.gov/pubmed/37370879
http://dx.doi.org/10.3390/diagnostics13121984
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