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Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action

Centipeda minima (CMX) has been widely investigated using network pharmacology and clinical studies for its effects on hair growth via the JAK/STAT signaling pathway. Human hair follicle papilla cells exhibit hair regrowth through the expression of Wnt signaling-related proteins. However, the mechan...

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Autores principales: Baek, Ji Yun, Kim, Byoung Ha, Kim, Dong-Wook, Lee, Won-Yung, Kim, Chang Eop, Kim, Hyun-Young, Pyo, Jaesung, Park, Eun-Seok, Kang, Ki Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297088/
https://www.ncbi.nlm.nih.gov/pubmed/37367071
http://dx.doi.org/10.3390/cimb45060322
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author Baek, Ji Yun
Kim, Byoung Ha
Kim, Dong-Wook
Lee, Won-Yung
Kim, Chang Eop
Kim, Hyun-Young
Pyo, Jaesung
Park, Eun-Seok
Kang, Ki Sung
author_facet Baek, Ji Yun
Kim, Byoung Ha
Kim, Dong-Wook
Lee, Won-Yung
Kim, Chang Eop
Kim, Hyun-Young
Pyo, Jaesung
Park, Eun-Seok
Kang, Ki Sung
author_sort Baek, Ji Yun
collection PubMed
description Centipeda minima (CMX) has been widely investigated using network pharmacology and clinical studies for its effects on hair growth via the JAK/STAT signaling pathway. Human hair follicle papilla cells exhibit hair regrowth through the expression of Wnt signaling-related proteins. However, the mechanism of action of CMX in animals has not been elucidated fully. This study examined the effect of induced hair loss and its side-effects on the skin, and observed the mechanism of action of an alcoholic extract of CMX (DN106212) on C57BL/6 mice. Our results showed that DN106212 was more effective in promoting hair growth than dimethyl sulfoxide in the negative control and tofacitinib (TF) in the positive control when mice were treated with DN106212 for 16 days. We confirmed that DN106212 promotes the formation of mature hair follicles through hematoxylin and eosin staining. We also found that the expression of vascular endothelial growth factor (Vegfa), insulin-like growth factor 1 (Igf1), and transforming growth factor beta 1 (Tgfb1) is related to hair growth using PCR. DN106212-treated mice had significantly higher expression of Vegfa and Igf1 than TF-treated ones, and inhibiting the expression of Tgfb1 had similar effects as TF treatment. In conclusion, we propose that DN106212 increases the expression of hair growth factors, promotes the development of hair follicles, and promotes hair growth. Although additional experiments are needed, DN106212 may serve as an experimental basis for research on natural hair growth-promoting agents.
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spelling pubmed-102970882023-06-28 Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action Baek, Ji Yun Kim, Byoung Ha Kim, Dong-Wook Lee, Won-Yung Kim, Chang Eop Kim, Hyun-Young Pyo, Jaesung Park, Eun-Seok Kang, Ki Sung Curr Issues Mol Biol Article Centipeda minima (CMX) has been widely investigated using network pharmacology and clinical studies for its effects on hair growth via the JAK/STAT signaling pathway. Human hair follicle papilla cells exhibit hair regrowth through the expression of Wnt signaling-related proteins. However, the mechanism of action of CMX in animals has not been elucidated fully. This study examined the effect of induced hair loss and its side-effects on the skin, and observed the mechanism of action of an alcoholic extract of CMX (DN106212) on C57BL/6 mice. Our results showed that DN106212 was more effective in promoting hair growth than dimethyl sulfoxide in the negative control and tofacitinib (TF) in the positive control when mice were treated with DN106212 for 16 days. We confirmed that DN106212 promotes the formation of mature hair follicles through hematoxylin and eosin staining. We also found that the expression of vascular endothelial growth factor (Vegfa), insulin-like growth factor 1 (Igf1), and transforming growth factor beta 1 (Tgfb1) is related to hair growth using PCR. DN106212-treated mice had significantly higher expression of Vegfa and Igf1 than TF-treated ones, and inhibiting the expression of Tgfb1 had similar effects as TF treatment. In conclusion, we propose that DN106212 increases the expression of hair growth factors, promotes the development of hair follicles, and promotes hair growth. Although additional experiments are needed, DN106212 may serve as an experimental basis for research on natural hair growth-promoting agents. MDPI 2023-06-09 /pmc/articles/PMC10297088/ /pubmed/37367071 http://dx.doi.org/10.3390/cimb45060322 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baek, Ji Yun
Kim, Byoung Ha
Kim, Dong-Wook
Lee, Won-Yung
Kim, Chang Eop
Kim, Hyun-Young
Pyo, Jaesung
Park, Eun-Seok
Kang, Ki Sung
Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action
title Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action
title_full Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action
title_fullStr Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action
title_full_unstemmed Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action
title_short Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action
title_sort hair growth effect of dn106212 in c57bl/6 mouse and its network pharmacological mechanism of action
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297088/
https://www.ncbi.nlm.nih.gov/pubmed/37367071
http://dx.doi.org/10.3390/cimb45060322
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