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A Systematic Review on Quiescent State Research Approaches in S. cerevisiae

Quiescence, the temporary and reversible arrest of cell growth, is a fundamental biological process. However, the lack of standardization in terms of reporting the experimental details of quiescent cells and populations can cause confusion and hinder knowledge transfer. We employ the systematic revi...

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Autores principales: Opalek, Monika, Tutaj, Hanna, Pirog, Adrian, Smug, Bogna J., Rutkowska, Joanna, Wloch-Salamon, Dominika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297366/
https://www.ncbi.nlm.nih.gov/pubmed/37371078
http://dx.doi.org/10.3390/cells12121608
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author Opalek, Monika
Tutaj, Hanna
Pirog, Adrian
Smug, Bogna J.
Rutkowska, Joanna
Wloch-Salamon, Dominika
author_facet Opalek, Monika
Tutaj, Hanna
Pirog, Adrian
Smug, Bogna J.
Rutkowska, Joanna
Wloch-Salamon, Dominika
author_sort Opalek, Monika
collection PubMed
description Quiescence, the temporary and reversible arrest of cell growth, is a fundamental biological process. However, the lack of standardization in terms of reporting the experimental details of quiescent cells and populations can cause confusion and hinder knowledge transfer. We employ the systematic review methodology to comprehensively analyze the diversity of approaches used to study the quiescent state, focusing on all published research addressing the budding yeast Saccharomyces cerevisiae. We group research articles into those that consider all cells comprising the stationary-phase (SP) population as quiescent and those that recognize heterogeneity within the SP by distinguishing phenotypically distinct subpopulations. Furthermore, we investigate the chronological age of the quiescent populations under study and the methods used to induce the quiescent state, such as gradual starvation or abrupt environmental change. We also assess whether the strains used in research are prototrophic or auxotrophic. By combining the above features, we identify 48 possible experimental setups that can be used to study quiescence, which can be misleading when drawing general conclusions. We therefore summarize our review by proposing guidelines and recommendations pertaining to the information included in research articles. We believe that more rigorous reporting on the features of quiescent populations will facilitate knowledge transfer within and between disciplines, thereby stimulating valuable scientific discussion.
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spelling pubmed-102973662023-06-28 A Systematic Review on Quiescent State Research Approaches in S. cerevisiae Opalek, Monika Tutaj, Hanna Pirog, Adrian Smug, Bogna J. Rutkowska, Joanna Wloch-Salamon, Dominika Cells Systematic Review Quiescence, the temporary and reversible arrest of cell growth, is a fundamental biological process. However, the lack of standardization in terms of reporting the experimental details of quiescent cells and populations can cause confusion and hinder knowledge transfer. We employ the systematic review methodology to comprehensively analyze the diversity of approaches used to study the quiescent state, focusing on all published research addressing the budding yeast Saccharomyces cerevisiae. We group research articles into those that consider all cells comprising the stationary-phase (SP) population as quiescent and those that recognize heterogeneity within the SP by distinguishing phenotypically distinct subpopulations. Furthermore, we investigate the chronological age of the quiescent populations under study and the methods used to induce the quiescent state, such as gradual starvation or abrupt environmental change. We also assess whether the strains used in research are prototrophic or auxotrophic. By combining the above features, we identify 48 possible experimental setups that can be used to study quiescence, which can be misleading when drawing general conclusions. We therefore summarize our review by proposing guidelines and recommendations pertaining to the information included in research articles. We believe that more rigorous reporting on the features of quiescent populations will facilitate knowledge transfer within and between disciplines, thereby stimulating valuable scientific discussion. MDPI 2023-06-12 /pmc/articles/PMC10297366/ /pubmed/37371078 http://dx.doi.org/10.3390/cells12121608 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Systematic Review
Opalek, Monika
Tutaj, Hanna
Pirog, Adrian
Smug, Bogna J.
Rutkowska, Joanna
Wloch-Salamon, Dominika
A Systematic Review on Quiescent State Research Approaches in S. cerevisiae
title A Systematic Review on Quiescent State Research Approaches in S. cerevisiae
title_full A Systematic Review on Quiescent State Research Approaches in S. cerevisiae
title_fullStr A Systematic Review on Quiescent State Research Approaches in S. cerevisiae
title_full_unstemmed A Systematic Review on Quiescent State Research Approaches in S. cerevisiae
title_short A Systematic Review on Quiescent State Research Approaches in S. cerevisiae
title_sort systematic review on quiescent state research approaches in s. cerevisiae
topic Systematic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297366/
https://www.ncbi.nlm.nih.gov/pubmed/37371078
http://dx.doi.org/10.3390/cells12121608
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