A high-performance electrochemical biosensor using an engineered urate oxidase

We constructed a high-performance biosensor for detecting uric acid by immobilizing an engineered urate oxidase on gold nanoparticles deposited on a carbon-glass electrode. This biosensor showed a low limit-of-detection (9.16 nM), a high sensitivity (14 μA/μM), a wide range of linearity (50 nM–1 mM)...

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Detalles Bibliográficos
Autores principales: Wei, Zheng, Knaus, Tanja, Liu, Yuxin, Zhai, Ziran, Gargano, Andrea F. G., Rothenberg, Gadi, Yan, Ning, Mutti, Francesco G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297830/
https://www.ncbi.nlm.nih.gov/pubmed/37285304
http://dx.doi.org/10.1039/d3cc01869e
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author Wei, Zheng
Knaus, Tanja
Liu, Yuxin
Zhai, Ziran
Gargano, Andrea F. G.
Rothenberg, Gadi
Yan, Ning
Mutti, Francesco G.
author_facet Wei, Zheng
Knaus, Tanja
Liu, Yuxin
Zhai, Ziran
Gargano, Andrea F. G.
Rothenberg, Gadi
Yan, Ning
Mutti, Francesco G.
author_sort Wei, Zheng
collection PubMed
description We constructed a high-performance biosensor for detecting uric acid by immobilizing an engineered urate oxidase on gold nanoparticles deposited on a carbon-glass electrode. This biosensor showed a low limit-of-detection (9.16 nM), a high sensitivity (14 μA/μM), a wide range of linearity (50 nM–1 mM), and more than 28 days lifetime.
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spelling pubmed-102978302023-06-28 A high-performance electrochemical biosensor using an engineered urate oxidase Wei, Zheng Knaus, Tanja Liu, Yuxin Zhai, Ziran Gargano, Andrea F. G. Rothenberg, Gadi Yan, Ning Mutti, Francesco G. Chem Commun (Camb) Chemistry We constructed a high-performance biosensor for detecting uric acid by immobilizing an engineered urate oxidase on gold nanoparticles deposited on a carbon-glass electrode. This biosensor showed a low limit-of-detection (9.16 nM), a high sensitivity (14 μA/μM), a wide range of linearity (50 nM–1 mM), and more than 28 days lifetime. The Royal Society of Chemistry 2023-05-30 /pmc/articles/PMC10297830/ /pubmed/37285304 http://dx.doi.org/10.1039/d3cc01869e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Wei, Zheng
Knaus, Tanja
Liu, Yuxin
Zhai, Ziran
Gargano, Andrea F. G.
Rothenberg, Gadi
Yan, Ning
Mutti, Francesco G.
A high-performance electrochemical biosensor using an engineered urate oxidase
title A high-performance electrochemical biosensor using an engineered urate oxidase
title_full A high-performance electrochemical biosensor using an engineered urate oxidase
title_fullStr A high-performance electrochemical biosensor using an engineered urate oxidase
title_full_unstemmed A high-performance electrochemical biosensor using an engineered urate oxidase
title_short A high-performance electrochemical biosensor using an engineered urate oxidase
title_sort high-performance electrochemical biosensor using an engineered urate oxidase
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10297830/
https://www.ncbi.nlm.nih.gov/pubmed/37285304
http://dx.doi.org/10.1039/d3cc01869e
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