Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant Sublineages

The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer...

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Detalles Bibliográficos
Autores principales: Esman, Anna, Dubodelov, Dmitry, Khafizov, Kamil, Kotov, Ivan, Roev, German, Golubeva, Anna, Gasanov, Gasan, Korabelnikova, Marina, Turashev, Askar, Cherkashin, Evgeniy, Mironov, Konstantin, Cherkashina, Anna, Akimkin, Vasily
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298075/
https://www.ncbi.nlm.nih.gov/pubmed/37372398
http://dx.doi.org/10.3390/genes14061218
Descripción
Sumario:The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer panels for RT-PCR and Sanger and NGS sequencing methods. For the centralized collection and analysis of samples, the VGARus database was developed, which currently includes more than 300,000 viral sequences.

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