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Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility

Industrial chicory (Cichorium intybus var. sativum) is a biannual crop mostly cultivated for extraction of inulin, a fructose polymer used as a dietary fiber. F1 hybrid breeding is a promising breeding strategy in chicory but relies on stable male sterile lines to prevent self-pollination. Here, we...

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Autores principales: Waegneer, Evelien, Rombauts, Stephane, Baert, Joost, Dauchot, Nicolas, De Keyser, Annick, Eeckhaut, Tom, Haegeman, Annelies, Liu, Chang, Maudoux, Olivier, Notté, Christine, Staelens, Ariane, Van der Veken, Jeroen, Van Laere, Katrijn, Ruttink, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298185/
https://www.ncbi.nlm.nih.gov/pubmed/37384353
http://dx.doi.org/10.3389/fpls.2023.1181529
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author Waegneer, Evelien
Rombauts, Stephane
Baert, Joost
Dauchot, Nicolas
De Keyser, Annick
Eeckhaut, Tom
Haegeman, Annelies
Liu, Chang
Maudoux, Olivier
Notté, Christine
Staelens, Ariane
Van der Veken, Jeroen
Van Laere, Katrijn
Ruttink, Tom
author_facet Waegneer, Evelien
Rombauts, Stephane
Baert, Joost
Dauchot, Nicolas
De Keyser, Annick
Eeckhaut, Tom
Haegeman, Annelies
Liu, Chang
Maudoux, Olivier
Notté, Christine
Staelens, Ariane
Van der Veken, Jeroen
Van Laere, Katrijn
Ruttink, Tom
author_sort Waegneer, Evelien
collection PubMed
description Industrial chicory (Cichorium intybus var. sativum) is a biannual crop mostly cultivated for extraction of inulin, a fructose polymer used as a dietary fiber. F1 hybrid breeding is a promising breeding strategy in chicory but relies on stable male sterile lines to prevent self-pollination. Here, we report the assembly and annotation of a new industrial chicory reference genome. Additionally, we performed RNA-Seq on subsequent stages of flower bud development of a fertile line and two cytoplasmic male sterile (CMS) clones. Comparison of fertile and CMS flower bud transcriptomes combined with morphological microscopic analysis of anthers, provided a molecular understanding of anther development and identified key genes in a range of underlying processes, including tapetum development, sink establishment, pollen wall development and anther dehiscence. We also described the role of phytohormones in the regulation of these processes under normal fertile flower bud development. In parallel, we evaluated which processes are disturbed in CMS clones and could contribute to the male sterile phenotype. Taken together, this study provides a state-of-the-art industrial chicory reference genome, an annotated and curated candidate gene set related to anther development and male sterility as well as a detailed molecular timetable of flower bud development in fertile and CMS lines.
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spelling pubmed-102981852023-06-28 Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility Waegneer, Evelien Rombauts, Stephane Baert, Joost Dauchot, Nicolas De Keyser, Annick Eeckhaut, Tom Haegeman, Annelies Liu, Chang Maudoux, Olivier Notté, Christine Staelens, Ariane Van der Veken, Jeroen Van Laere, Katrijn Ruttink, Tom Front Plant Sci Plant Science Industrial chicory (Cichorium intybus var. sativum) is a biannual crop mostly cultivated for extraction of inulin, a fructose polymer used as a dietary fiber. F1 hybrid breeding is a promising breeding strategy in chicory but relies on stable male sterile lines to prevent self-pollination. Here, we report the assembly and annotation of a new industrial chicory reference genome. Additionally, we performed RNA-Seq on subsequent stages of flower bud development of a fertile line and two cytoplasmic male sterile (CMS) clones. Comparison of fertile and CMS flower bud transcriptomes combined with morphological microscopic analysis of anthers, provided a molecular understanding of anther development and identified key genes in a range of underlying processes, including tapetum development, sink establishment, pollen wall development and anther dehiscence. We also described the role of phytohormones in the regulation of these processes under normal fertile flower bud development. In parallel, we evaluated which processes are disturbed in CMS clones and could contribute to the male sterile phenotype. Taken together, this study provides a state-of-the-art industrial chicory reference genome, an annotated and curated candidate gene set related to anther development and male sterility as well as a detailed molecular timetable of flower bud development in fertile and CMS lines. Frontiers Media S.A. 2023-06-13 /pmc/articles/PMC10298185/ /pubmed/37384353 http://dx.doi.org/10.3389/fpls.2023.1181529 Text en Copyright © 2023 Waegneer, Rombauts, Baert, Dauchot, De Keyser, Eeckhaut, Haegeman, Liu, Maudoux, Notté, Staelens, Van der Veken, Van Laere and Ruttink https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Waegneer, Evelien
Rombauts, Stephane
Baert, Joost
Dauchot, Nicolas
De Keyser, Annick
Eeckhaut, Tom
Haegeman, Annelies
Liu, Chang
Maudoux, Olivier
Notté, Christine
Staelens, Ariane
Van der Veken, Jeroen
Van Laere, Katrijn
Ruttink, Tom
Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility
title Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility
title_full Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility
title_fullStr Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility
title_full_unstemmed Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility
title_short Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility
title_sort industrial chicory genome gives insights into the molecular timetable of anther development and male sterility
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298185/
https://www.ncbi.nlm.nih.gov/pubmed/37384353
http://dx.doi.org/10.3389/fpls.2023.1181529
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