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The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database

Tuberculosis (TB) is a multisystemic contagious disease produced by Mycobacterium tuberculosis complex bacteria (MTBC), with a prevalence of 65:100,000 inhabitants in Romania (six times higher than the European average). The diagnosis usually relies on the detection of MTBC in culture. Although this...

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Autores principales: Sava, Cristian, Sava, Mihaela, Drăgan, Ana-Maria, Iuhas, Alin, Niulaș, Larisa, Marinău, Cristian Phillip, Balmoș, Andreea Bianca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298219/
https://www.ncbi.nlm.nih.gov/pubmed/37372411
http://dx.doi.org/10.3390/genes14061231
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author Sava, Cristian
Sava, Mihaela
Drăgan, Ana-Maria
Iuhas, Alin
Niulaș, Larisa
Marinău, Cristian Phillip
Balmoș, Andreea Bianca
author_facet Sava, Cristian
Sava, Mihaela
Drăgan, Ana-Maria
Iuhas, Alin
Niulaș, Larisa
Marinău, Cristian Phillip
Balmoș, Andreea Bianca
author_sort Sava, Cristian
collection PubMed
description Tuberculosis (TB) is a multisystemic contagious disease produced by Mycobacterium tuberculosis complex bacteria (MTBC), with a prevalence of 65:100,000 inhabitants in Romania (six times higher than the European average). The diagnosis usually relies on the detection of MTBC in culture. Although this is a sensitive method of detection and remains the “gold standard”, the results are obtained after several weeks. Nucleic acid amplification tests (NAATs), being a quick and sensitive method, represent progress in the diagnosis of TB. The aim of this study is to assess the assumption that NAAT using Xpert MTB/RIF is an efficient method of TB diagnosis and has the capacity to reduce false-positive results. Pathological samples from 862 patients with TB suspicion were tested using microscopic examination, molecular testing and bacterial culture. The results show that the Xpert MTB/RIF Ultra test has a sensitivity of 95% and a specificity of 96.4% compared with 54.8% sensitivity and 99.5% specificity for Ziehl–Neelsen stain microscopy, and an average of 30 days gained in the diagnosis of TB compared with bacterial culture. The implementation of molecular testing in TB laboratories leads to an important increase in early diagnostics of the disease and the prompter isolation and treatment of infected patients.
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spelling pubmed-102982192023-06-28 The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database Sava, Cristian Sava, Mihaela Drăgan, Ana-Maria Iuhas, Alin Niulaș, Larisa Marinău, Cristian Phillip Balmoș, Andreea Bianca Genes (Basel) Article Tuberculosis (TB) is a multisystemic contagious disease produced by Mycobacterium tuberculosis complex bacteria (MTBC), with a prevalence of 65:100,000 inhabitants in Romania (six times higher than the European average). The diagnosis usually relies on the detection of MTBC in culture. Although this is a sensitive method of detection and remains the “gold standard”, the results are obtained after several weeks. Nucleic acid amplification tests (NAATs), being a quick and sensitive method, represent progress in the diagnosis of TB. The aim of this study is to assess the assumption that NAAT using Xpert MTB/RIF is an efficient method of TB diagnosis and has the capacity to reduce false-positive results. Pathological samples from 862 patients with TB suspicion were tested using microscopic examination, molecular testing and bacterial culture. The results show that the Xpert MTB/RIF Ultra test has a sensitivity of 95% and a specificity of 96.4% compared with 54.8% sensitivity and 99.5% specificity for Ziehl–Neelsen stain microscopy, and an average of 30 days gained in the diagnosis of TB compared with bacterial culture. The implementation of molecular testing in TB laboratories leads to an important increase in early diagnostics of the disease and the prompter isolation and treatment of infected patients. MDPI 2023-06-07 /pmc/articles/PMC10298219/ /pubmed/37372411 http://dx.doi.org/10.3390/genes14061231 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sava, Cristian
Sava, Mihaela
Drăgan, Ana-Maria
Iuhas, Alin
Niulaș, Larisa
Marinău, Cristian Phillip
Balmoș, Andreea Bianca
The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database
title The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database
title_full The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database
title_fullStr The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database
title_full_unstemmed The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database
title_short The Use of Xpert MTB/RIF Ultra Testing for Early Diagnosis of Tuberculosis: A Retrospective Study from a Single-Center Database
title_sort use of xpert mtb/rif ultra testing for early diagnosis of tuberculosis: a retrospective study from a single-center database
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298219/
https://www.ncbi.nlm.nih.gov/pubmed/37372411
http://dx.doi.org/10.3390/genes14061231
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