Cargando…

Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism

BAHD acyltransferases (BAHDs), especially those present in plant epidermal wax metabolism, are crucial for environmental adaptation. Epidermal waxes primarily comprise very-long-chain fatty acids (VLCFAs) and their derivatives, serving as significant components of aboveground plant organs. These wax...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Lecheng, Xu, Huanhuan, Zhang, Wanyue, Xing, Jiayi, Zhu, Mingzhao, Zhang, Yuchen, Wang, Yongqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298236/
https://www.ncbi.nlm.nih.gov/pubmed/37372466
http://dx.doi.org/10.3390/genes14061286
_version_ 1785064065507262464
author Liu, Lecheng
Xu, Huanhuan
Zhang, Wanyue
Xing, Jiayi
Zhu, Mingzhao
Zhang, Yuchen
Wang, Yongqin
author_facet Liu, Lecheng
Xu, Huanhuan
Zhang, Wanyue
Xing, Jiayi
Zhu, Mingzhao
Zhang, Yuchen
Wang, Yongqin
author_sort Liu, Lecheng
collection PubMed
description BAHD acyltransferases (BAHDs), especially those present in plant epidermal wax metabolism, are crucial for environmental adaptation. Epidermal waxes primarily comprise very-long-chain fatty acids (VLCFAs) and their derivatives, serving as significant components of aboveground plant organs. These waxes play an essential role in resisting biotic and abiotic stresses. In this study, we identified the BAHD family in Welsh onion (Allium fistulosum). Our analysis revealed the presence of AfBAHDs in all chromosomes, with a distinct concentration in Chr3. Furthermore, the cis-acting elements of AfBAHDs were associated with abiotic/biotic stress, hormones, and light. The motif of Welsh onion BAHDs indicated the presence of a specific BAHDs motif. We also established the phylogenetic relationships of AfBAHDs, identifying three homologous genes of CER2. Subsequently, we characterized the expression of AfCER2-LIKEs in a Welsh onion mutant deficient in wax and found that AfCER2-LIKE1 plays a critical role in leaf wax metabolism, while all AfCER2-LIKEs respond to abiotic stress. Our findings provide new insights into the BAHD family and lay a foundation for future studies on the regulation of wax metabolism in Welsh onion.
format Online
Article
Text
id pubmed-10298236
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102982362023-06-28 Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism Liu, Lecheng Xu, Huanhuan Zhang, Wanyue Xing, Jiayi Zhu, Mingzhao Zhang, Yuchen Wang, Yongqin Genes (Basel) Article BAHD acyltransferases (BAHDs), especially those present in plant epidermal wax metabolism, are crucial for environmental adaptation. Epidermal waxes primarily comprise very-long-chain fatty acids (VLCFAs) and their derivatives, serving as significant components of aboveground plant organs. These waxes play an essential role in resisting biotic and abiotic stresses. In this study, we identified the BAHD family in Welsh onion (Allium fistulosum). Our analysis revealed the presence of AfBAHDs in all chromosomes, with a distinct concentration in Chr3. Furthermore, the cis-acting elements of AfBAHDs were associated with abiotic/biotic stress, hormones, and light. The motif of Welsh onion BAHDs indicated the presence of a specific BAHDs motif. We also established the phylogenetic relationships of AfBAHDs, identifying three homologous genes of CER2. Subsequently, we characterized the expression of AfCER2-LIKEs in a Welsh onion mutant deficient in wax and found that AfCER2-LIKE1 plays a critical role in leaf wax metabolism, while all AfCER2-LIKEs respond to abiotic stress. Our findings provide new insights into the BAHD family and lay a foundation for future studies on the regulation of wax metabolism in Welsh onion. MDPI 2023-06-18 /pmc/articles/PMC10298236/ /pubmed/37372466 http://dx.doi.org/10.3390/genes14061286 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Lecheng
Xu, Huanhuan
Zhang, Wanyue
Xing, Jiayi
Zhu, Mingzhao
Zhang, Yuchen
Wang, Yongqin
Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism
title Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism
title_full Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism
title_fullStr Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism
title_full_unstemmed Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism
title_short Genome-Wide Analysis of the BAHD Family in Welsh Onion and CER2-LIKEs Involved in Wax Metabolism
title_sort genome-wide analysis of the bahd family in welsh onion and cer2-likes involved in wax metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298236/
https://www.ncbi.nlm.nih.gov/pubmed/37372466
http://dx.doi.org/10.3390/genes14061286
work_keys_str_mv AT liulecheng genomewideanalysisofthebahdfamilyinwelshonionandcer2likesinvolvedinwaxmetabolism
AT xuhuanhuan genomewideanalysisofthebahdfamilyinwelshonionandcer2likesinvolvedinwaxmetabolism
AT zhangwanyue genomewideanalysisofthebahdfamilyinwelshonionandcer2likesinvolvedinwaxmetabolism
AT xingjiayi genomewideanalysisofthebahdfamilyinwelshonionandcer2likesinvolvedinwaxmetabolism
AT zhumingzhao genomewideanalysisofthebahdfamilyinwelshonionandcer2likesinvolvedinwaxmetabolism
AT zhangyuchen genomewideanalysisofthebahdfamilyinwelshonionandcer2likesinvolvedinwaxmetabolism
AT wangyongqin genomewideanalysisofthebahdfamilyinwelshonionandcer2likesinvolvedinwaxmetabolism