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Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A

Jujubosides are the major medicinal ingredients of Ziziphi Spinosae Semen (the seed of wild jujube). To date, a complete understanding of jujuboside’s metabolic pathways has not been attained. This study has systematically identified 35 β-glucosidase genes belonging to the glycoside hydrolase family...

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Autores principales: Yang, Mingjun, Ma, Yimian, Si, Xupeng, Liu, Xiaofeng, Geng, Xin, Wen, Xin, Li, Guoqiong, Zhang, Liping, Yang, Chengmin, Zhang, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298349/
https://www.ncbi.nlm.nih.gov/pubmed/37372316
http://dx.doi.org/10.3390/genes14061135
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author Yang, Mingjun
Ma, Yimian
Si, Xupeng
Liu, Xiaofeng
Geng, Xin
Wen, Xin
Li, Guoqiong
Zhang, Liping
Yang, Chengmin
Zhang, Zheng
author_facet Yang, Mingjun
Ma, Yimian
Si, Xupeng
Liu, Xiaofeng
Geng, Xin
Wen, Xin
Li, Guoqiong
Zhang, Liping
Yang, Chengmin
Zhang, Zheng
author_sort Yang, Mingjun
collection PubMed
description Jujubosides are the major medicinal ingredients of Ziziphi Spinosae Semen (the seed of wild jujube). To date, a complete understanding of jujuboside’s metabolic pathways has not been attained. This study has systematically identified 35 β-glucosidase genes belonging to the glycoside hydrolase family 1 (GH1) using bioinformatic methods based on the wild jujube genome. The conserved domains and motifs of the 35 putative β-glucosidases, along with the genome locations and exon–intron structures of 35 β-glucosidase genes were revealed. The potential functions of the putative proteins encoded by the 35 β-glucosidase genes are suggested based on their phylogenetic relationships with Arabidopsis homologs. Two wild jujube β-glucosidase genes were heterologously expressed in Escherichia coli, and the recombinant proteins were able to convert jujuboside A (JuA) into jujuboside B (JuB). Since it has been previously reported that JuA catabolites, including JuB and other rare jujubosides, may play crucial roles in the jujuboside’s pharmacological activity, it is suggested that these two proteins can be used to enhance the utilization potential of jujubosides. This study provides new insight into the metabolism of jujubosides in wild jujube. Furthermore, the characterization of β-glucosidase genes is expected to facilitate investigations involving the cultivation and breeding of wild jujube.
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spelling pubmed-102983492023-06-28 Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A Yang, Mingjun Ma, Yimian Si, Xupeng Liu, Xiaofeng Geng, Xin Wen, Xin Li, Guoqiong Zhang, Liping Yang, Chengmin Zhang, Zheng Genes (Basel) Article Jujubosides are the major medicinal ingredients of Ziziphi Spinosae Semen (the seed of wild jujube). To date, a complete understanding of jujuboside’s metabolic pathways has not been attained. This study has systematically identified 35 β-glucosidase genes belonging to the glycoside hydrolase family 1 (GH1) using bioinformatic methods based on the wild jujube genome. The conserved domains and motifs of the 35 putative β-glucosidases, along with the genome locations and exon–intron structures of 35 β-glucosidase genes were revealed. The potential functions of the putative proteins encoded by the 35 β-glucosidase genes are suggested based on their phylogenetic relationships with Arabidopsis homologs. Two wild jujube β-glucosidase genes were heterologously expressed in Escherichia coli, and the recombinant proteins were able to convert jujuboside A (JuA) into jujuboside B (JuB). Since it has been previously reported that JuA catabolites, including JuB and other rare jujubosides, may play crucial roles in the jujuboside’s pharmacological activity, it is suggested that these two proteins can be used to enhance the utilization potential of jujubosides. This study provides new insight into the metabolism of jujubosides in wild jujube. Furthermore, the characterization of β-glucosidase genes is expected to facilitate investigations involving the cultivation and breeding of wild jujube. MDPI 2023-05-24 /pmc/articles/PMC10298349/ /pubmed/37372316 http://dx.doi.org/10.3390/genes14061135 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Mingjun
Ma, Yimian
Si, Xupeng
Liu, Xiaofeng
Geng, Xin
Wen, Xin
Li, Guoqiong
Zhang, Liping
Yang, Chengmin
Zhang, Zheng
Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A
title Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A
title_full Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A
title_fullStr Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A
title_full_unstemmed Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A
title_short Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A
title_sort analysis of the glycoside hydrolase family 1 from wild jujube reveals genes involved in the degradation of jujuboside a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298349/
https://www.ncbi.nlm.nih.gov/pubmed/37372316
http://dx.doi.org/10.3390/genes14061135
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