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Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation
Cell identity is determined by the chromatin structure and profiles of gene expression, which are dependent on chromatin accessibility and DNA methylation of the regions critical for gene expression, such as enhancers and promoters. These epigenetic modifications are required for mammalian developme...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298370/ https://www.ncbi.nlm.nih.gov/pubmed/37373098 http://dx.doi.org/10.3390/ijms24129951 |
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author | Improda, Teresa Morgera, Valentina Vitale, Maria Chiariotti, Lorenzo Passaro, Fabiana Feola, Antonia Porcellini, Antonio Cuomo, Mariella Pezone, Antonio |
author_facet | Improda, Teresa Morgera, Valentina Vitale, Maria Chiariotti, Lorenzo Passaro, Fabiana Feola, Antonia Porcellini, Antonio Cuomo, Mariella Pezone, Antonio |
author_sort | Improda, Teresa |
collection | PubMed |
description | Cell identity is determined by the chromatin structure and profiles of gene expression, which are dependent on chromatin accessibility and DNA methylation of the regions critical for gene expression, such as enhancers and promoters. These epigenetic modifications are required for mammalian development and are essential for the establishment and maintenance of the cellular identity. DNA methylation was once thought to be a permanent repressive epigenetic mark, but systematic analyses in various genomic contexts have revealed a more dynamic regulation than previously thought. In fact, both active DNA methylation and demethylation occur during cell fate commitment and terminal differentiation. To link methylation signatures of specific genes to their expression profiles, we determined the methyl-CpG configurations of the promoters of five genes switched on and off during murine postnatal brain differentiation by bisulfite-targeted sequencing. Here, we report the structure of significant, dynamic, and stable methyl-CpG profiles associated with silencing or activation of the expression of genes during neural stem cell and brain postnatal differentiation. Strikingly, these methylation cores mark different mouse brain areas and cell types derived from the same areas during differentiation. |
format | Online Article Text |
id | pubmed-10298370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102983702023-06-28 Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation Improda, Teresa Morgera, Valentina Vitale, Maria Chiariotti, Lorenzo Passaro, Fabiana Feola, Antonia Porcellini, Antonio Cuomo, Mariella Pezone, Antonio Int J Mol Sci Article Cell identity is determined by the chromatin structure and profiles of gene expression, which are dependent on chromatin accessibility and DNA methylation of the regions critical for gene expression, such as enhancers and promoters. These epigenetic modifications are required for mammalian development and are essential for the establishment and maintenance of the cellular identity. DNA methylation was once thought to be a permanent repressive epigenetic mark, but systematic analyses in various genomic contexts have revealed a more dynamic regulation than previously thought. In fact, both active DNA methylation and demethylation occur during cell fate commitment and terminal differentiation. To link methylation signatures of specific genes to their expression profiles, we determined the methyl-CpG configurations of the promoters of five genes switched on and off during murine postnatal brain differentiation by bisulfite-targeted sequencing. Here, we report the structure of significant, dynamic, and stable methyl-CpG profiles associated with silencing or activation of the expression of genes during neural stem cell and brain postnatal differentiation. Strikingly, these methylation cores mark different mouse brain areas and cell types derived from the same areas during differentiation. MDPI 2023-06-09 /pmc/articles/PMC10298370/ /pubmed/37373098 http://dx.doi.org/10.3390/ijms24129951 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Improda, Teresa Morgera, Valentina Vitale, Maria Chiariotti, Lorenzo Passaro, Fabiana Feola, Antonia Porcellini, Antonio Cuomo, Mariella Pezone, Antonio Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation |
title | Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation |
title_full | Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation |
title_fullStr | Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation |
title_full_unstemmed | Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation |
title_short | Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation |
title_sort | specific methyl-cpg configurations define cell identity through gene expression regulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298370/ https://www.ncbi.nlm.nih.gov/pubmed/37373098 http://dx.doi.org/10.3390/ijms24129951 |
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