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Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation

Cell identity is determined by the chromatin structure and profiles of gene expression, which are dependent on chromatin accessibility and DNA methylation of the regions critical for gene expression, such as enhancers and promoters. These epigenetic modifications are required for mammalian developme...

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Autores principales: Improda, Teresa, Morgera, Valentina, Vitale, Maria, Chiariotti, Lorenzo, Passaro, Fabiana, Feola, Antonia, Porcellini, Antonio, Cuomo, Mariella, Pezone, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298370/
https://www.ncbi.nlm.nih.gov/pubmed/37373098
http://dx.doi.org/10.3390/ijms24129951
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author Improda, Teresa
Morgera, Valentina
Vitale, Maria
Chiariotti, Lorenzo
Passaro, Fabiana
Feola, Antonia
Porcellini, Antonio
Cuomo, Mariella
Pezone, Antonio
author_facet Improda, Teresa
Morgera, Valentina
Vitale, Maria
Chiariotti, Lorenzo
Passaro, Fabiana
Feola, Antonia
Porcellini, Antonio
Cuomo, Mariella
Pezone, Antonio
author_sort Improda, Teresa
collection PubMed
description Cell identity is determined by the chromatin structure and profiles of gene expression, which are dependent on chromatin accessibility and DNA methylation of the regions critical for gene expression, such as enhancers and promoters. These epigenetic modifications are required for mammalian development and are essential for the establishment and maintenance of the cellular identity. DNA methylation was once thought to be a permanent repressive epigenetic mark, but systematic analyses in various genomic contexts have revealed a more dynamic regulation than previously thought. In fact, both active DNA methylation and demethylation occur during cell fate commitment and terminal differentiation. To link methylation signatures of specific genes to their expression profiles, we determined the methyl-CpG configurations of the promoters of five genes switched on and off during murine postnatal brain differentiation by bisulfite-targeted sequencing. Here, we report the structure of significant, dynamic, and stable methyl-CpG profiles associated with silencing or activation of the expression of genes during neural stem cell and brain postnatal differentiation. Strikingly, these methylation cores mark different mouse brain areas and cell types derived from the same areas during differentiation.
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spelling pubmed-102983702023-06-28 Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation Improda, Teresa Morgera, Valentina Vitale, Maria Chiariotti, Lorenzo Passaro, Fabiana Feola, Antonia Porcellini, Antonio Cuomo, Mariella Pezone, Antonio Int J Mol Sci Article Cell identity is determined by the chromatin structure and profiles of gene expression, which are dependent on chromatin accessibility and DNA methylation of the regions critical for gene expression, such as enhancers and promoters. These epigenetic modifications are required for mammalian development and are essential for the establishment and maintenance of the cellular identity. DNA methylation was once thought to be a permanent repressive epigenetic mark, but systematic analyses in various genomic contexts have revealed a more dynamic regulation than previously thought. In fact, both active DNA methylation and demethylation occur during cell fate commitment and terminal differentiation. To link methylation signatures of specific genes to their expression profiles, we determined the methyl-CpG configurations of the promoters of five genes switched on and off during murine postnatal brain differentiation by bisulfite-targeted sequencing. Here, we report the structure of significant, dynamic, and stable methyl-CpG profiles associated with silencing or activation of the expression of genes during neural stem cell and brain postnatal differentiation. Strikingly, these methylation cores mark different mouse brain areas and cell types derived from the same areas during differentiation. MDPI 2023-06-09 /pmc/articles/PMC10298370/ /pubmed/37373098 http://dx.doi.org/10.3390/ijms24129951 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Improda, Teresa
Morgera, Valentina
Vitale, Maria
Chiariotti, Lorenzo
Passaro, Fabiana
Feola, Antonia
Porcellini, Antonio
Cuomo, Mariella
Pezone, Antonio
Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation
title Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation
title_full Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation
title_fullStr Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation
title_full_unstemmed Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation
title_short Specific Methyl-CpG Configurations Define Cell Identity through Gene Expression Regulation
title_sort specific methyl-cpg configurations define cell identity through gene expression regulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298370/
https://www.ncbi.nlm.nih.gov/pubmed/37373098
http://dx.doi.org/10.3390/ijms24129951
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