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Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation

One of the strategies to overcome diseases or abiotic stress in crops is the use of improved varieties. Genetic improvement could be accomplished through different methods, including conventional breeding, induced mutation, genetic transformation, or gene editing. The gene function and regulated exp...

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Autores principales: Villao-Uzho, Liliana, Chávez-Navarrete, Tatiana, Pacheco-Coello, Ricardo, Sánchez-Timm, Eduardo, Santos-Ordóñez, Efrén
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298551/
https://www.ncbi.nlm.nih.gov/pubmed/37372407
http://dx.doi.org/10.3390/genes14061226
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author Villao-Uzho, Liliana
Chávez-Navarrete, Tatiana
Pacheco-Coello, Ricardo
Sánchez-Timm, Eduardo
Santos-Ordóñez, Efrén
author_facet Villao-Uzho, Liliana
Chávez-Navarrete, Tatiana
Pacheco-Coello, Ricardo
Sánchez-Timm, Eduardo
Santos-Ordóñez, Efrén
author_sort Villao-Uzho, Liliana
collection PubMed
description One of the strategies to overcome diseases or abiotic stress in crops is the use of improved varieties. Genetic improvement could be accomplished through different methods, including conventional breeding, induced mutation, genetic transformation, or gene editing. The gene function and regulated expression through promoters are necessary for transgenic crops to improve specific traits. The variety of promoter sequences has increased in the generation of genetically modified crops because they could lead to the expression of the gene responsible for the improved trait in a specific manner. Therefore, the characterization of the promoter activity is necessary for the generation of biotechnological crops. That is why several analyses have focused on identifying and isolating promoters using techniques such as reverse transcriptase-polymerase chain reaction (RT-PCR), genetic libraries, cloning, and sequencing. Promoter analysis involves the plant genetic transformation method, a potent tool for determining the promoter activity and function of genes in plants, contributing to understanding gene regulation and plant development. Furthermore, the study of promoters that play a fundamental role in gene regulation is highly relevant. The study of regulation and development in transgenic organisms has made it possible to understand the benefits of directing gene expression in a temporal, spatial, and even controlled manner, confirming the great diversity of promoters discovered and developed. Therefore, promoters are a crucial tool in biotechnological processes to ensure the correct expression of a gene. This review highlights various types of promoters and their functionality in the generation of genetically modified crops.
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spelling pubmed-102985512023-06-28 Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation Villao-Uzho, Liliana Chávez-Navarrete, Tatiana Pacheco-Coello, Ricardo Sánchez-Timm, Eduardo Santos-Ordóñez, Efrén Genes (Basel) Review One of the strategies to overcome diseases or abiotic stress in crops is the use of improved varieties. Genetic improvement could be accomplished through different methods, including conventional breeding, induced mutation, genetic transformation, or gene editing. The gene function and regulated expression through promoters are necessary for transgenic crops to improve specific traits. The variety of promoter sequences has increased in the generation of genetically modified crops because they could lead to the expression of the gene responsible for the improved trait in a specific manner. Therefore, the characterization of the promoter activity is necessary for the generation of biotechnological crops. That is why several analyses have focused on identifying and isolating promoters using techniques such as reverse transcriptase-polymerase chain reaction (RT-PCR), genetic libraries, cloning, and sequencing. Promoter analysis involves the plant genetic transformation method, a potent tool for determining the promoter activity and function of genes in plants, contributing to understanding gene regulation and plant development. Furthermore, the study of promoters that play a fundamental role in gene regulation is highly relevant. The study of regulation and development in transgenic organisms has made it possible to understand the benefits of directing gene expression in a temporal, spatial, and even controlled manner, confirming the great diversity of promoters discovered and developed. Therefore, promoters are a crucial tool in biotechnological processes to ensure the correct expression of a gene. This review highlights various types of promoters and their functionality in the generation of genetically modified crops. MDPI 2023-06-06 /pmc/articles/PMC10298551/ /pubmed/37372407 http://dx.doi.org/10.3390/genes14061226 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Villao-Uzho, Liliana
Chávez-Navarrete, Tatiana
Pacheco-Coello, Ricardo
Sánchez-Timm, Eduardo
Santos-Ordóñez, Efrén
Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation
title Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation
title_full Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation
title_fullStr Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation
title_full_unstemmed Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation
title_short Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation
title_sort plant promoters: their identification, characterization, and role in gene regulation
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298551/
https://www.ncbi.nlm.nih.gov/pubmed/37372407
http://dx.doi.org/10.3390/genes14061226
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