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A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding

A collection of 30 melon introgression lines (ILs) was developed from the wild accession Ames 24297 (TRI) into ‘Piel de Sapo’ (PS) genetic background. Each IL carried an average of 1.4 introgressions from TRI, and the introgressions represented 91.4% of the TRI genome. Twenty-two ILs, representing 7...

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Autores principales: Campos, Manuel, Gonzalo, Maria José, Díaz, Aurora, Picó, Belén, Gómez-Guillamón, Maria Luisa, Monforte, Antonio José, Esteras, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298652/
https://www.ncbi.nlm.nih.gov/pubmed/37373247
http://dx.doi.org/10.3390/ijms241210099
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author Campos, Manuel
Gonzalo, Maria José
Díaz, Aurora
Picó, Belén
Gómez-Guillamón, Maria Luisa
Monforte, Antonio José
Esteras, Cristina
author_facet Campos, Manuel
Gonzalo, Maria José
Díaz, Aurora
Picó, Belén
Gómez-Guillamón, Maria Luisa
Monforte, Antonio José
Esteras, Cristina
author_sort Campos, Manuel
collection PubMed
description A collection of 30 melon introgression lines (ILs) was developed from the wild accession Ames 24297 (TRI) into ‘Piel de Sapo’ (PS) genetic background. Each IL carried an average of 1.4 introgressions from TRI, and the introgressions represented 91.4% of the TRI genome. Twenty-two ILs, representing 75% of the TRI genome, were evaluated in greenhouse (Algarrobo and Meliana) and field (Alcàsser) trials, mainly to study traits related to domestication syndrome such as fruit weight (FW) and flesh content (FFP), as well as other fruit quality traits as fruit shape (FS), flesh firmness (FF), soluble solid concentration (SSC), rind color and abscission layer. The IL collection showed an impressive variation in size-related traits, with FW ranging from 800 to 4100 g, reflecting the strong effect of the wild genome on these traits. Most of the ILs produced smaller fruits compared with PS; however, unexpectedly, the IL TRI05-2 produced bigger fruits, likely due to new epistatic interacions with the PS genetic background. In contrast, the genotypic effect for FS was smaller, and few QTLs with notable effects were detected. Interestingly, variability was also observed for FFP, FF and SSC, rind color and abscission layer formation. Genes in these introgressions are candidates for having been involved in melon domestication and diversification as well. These results confirm that the TRI IL collection is a very powerful tool for mapping traits of agronomic interest in melon, allowing the confirmation of previously reported QTLs and the identification of new ones to better understand the domestication process of this crop.
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spelling pubmed-102986522023-06-28 A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding Campos, Manuel Gonzalo, Maria José Díaz, Aurora Picó, Belén Gómez-Guillamón, Maria Luisa Monforte, Antonio José Esteras, Cristina Int J Mol Sci Article A collection of 30 melon introgression lines (ILs) was developed from the wild accession Ames 24297 (TRI) into ‘Piel de Sapo’ (PS) genetic background. Each IL carried an average of 1.4 introgressions from TRI, and the introgressions represented 91.4% of the TRI genome. Twenty-two ILs, representing 75% of the TRI genome, were evaluated in greenhouse (Algarrobo and Meliana) and field (Alcàsser) trials, mainly to study traits related to domestication syndrome such as fruit weight (FW) and flesh content (FFP), as well as other fruit quality traits as fruit shape (FS), flesh firmness (FF), soluble solid concentration (SSC), rind color and abscission layer. The IL collection showed an impressive variation in size-related traits, with FW ranging from 800 to 4100 g, reflecting the strong effect of the wild genome on these traits. Most of the ILs produced smaller fruits compared with PS; however, unexpectedly, the IL TRI05-2 produced bigger fruits, likely due to new epistatic interacions with the PS genetic background. In contrast, the genotypic effect for FS was smaller, and few QTLs with notable effects were detected. Interestingly, variability was also observed for FFP, FF and SSC, rind color and abscission layer formation. Genes in these introgressions are candidates for having been involved in melon domestication and diversification as well. These results confirm that the TRI IL collection is a very powerful tool for mapping traits of agronomic interest in melon, allowing the confirmation of previously reported QTLs and the identification of new ones to better understand the domestication process of this crop. MDPI 2023-06-14 /pmc/articles/PMC10298652/ /pubmed/37373247 http://dx.doi.org/10.3390/ijms241210099 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Campos, Manuel
Gonzalo, Maria José
Díaz, Aurora
Picó, Belén
Gómez-Guillamón, Maria Luisa
Monforte, Antonio José
Esteras, Cristina
A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding
title A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding
title_full A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding
title_fullStr A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding
title_full_unstemmed A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding
title_short A Novel Introgression Line Library Derived from a Wild Melon Gives Insights into the Genetics of Melon Domestication, Uncovering New Genetic Variability Useful for Breeding
title_sort novel introgression line library derived from a wild melon gives insights into the genetics of melon domestication, uncovering new genetic variability useful for breeding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298652/
https://www.ncbi.nlm.nih.gov/pubmed/37373247
http://dx.doi.org/10.3390/ijms241210099
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