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Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study

Background: Specific polymorphisms might influence controlled ovarian stimulation in women undergoing assisted reproductive technologies (ARTs). Data regarding possible interactions of these polymorphisms are still scanty. The aim of this analysis was to evaluate the effect of polymorphisms of gonad...

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Autores principales: Alviggi, Carlo, Longobardi, Salvatore, Papaleo, Enrico, Santi, Daniele, Alfano, Simona, Vanni, Valeria Stella, Campitiello, Maria Rosaria, De Rosa, Pasquale, Strina, Ida, Huhtaniemi, Ilpo, Pursiheimo, Juha-Pekka, D’Hooghe, Thomas, Humaidan, Peter, Conforti, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298688/
https://www.ncbi.nlm.nih.gov/pubmed/37372449
http://dx.doi.org/10.3390/genes14061269
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author Alviggi, Carlo
Longobardi, Salvatore
Papaleo, Enrico
Santi, Daniele
Alfano, Simona
Vanni, Valeria Stella
Campitiello, Maria Rosaria
De Rosa, Pasquale
Strina, Ida
Huhtaniemi, Ilpo
Pursiheimo, Juha-Pekka
D’Hooghe, Thomas
Humaidan, Peter
Conforti, Alessandro
author_facet Alviggi, Carlo
Longobardi, Salvatore
Papaleo, Enrico
Santi, Daniele
Alfano, Simona
Vanni, Valeria Stella
Campitiello, Maria Rosaria
De Rosa, Pasquale
Strina, Ida
Huhtaniemi, Ilpo
Pursiheimo, Juha-Pekka
D’Hooghe, Thomas
Humaidan, Peter
Conforti, Alessandro
author_sort Alviggi, Carlo
collection PubMed
description Background: Specific polymorphisms might influence controlled ovarian stimulation in women undergoing assisted reproductive technologies (ARTs). Data regarding possible interactions of these polymorphisms are still scanty. The aim of this analysis was to evaluate the effect of polymorphisms of gonadotropins and their receptors in women undergoing ART. Methods: A total of 94 normogonadotropic patients from three public ART units were enrolled. Patients underwent a gonadotropin releasing hormone (GnRH) long down-regulation protocol with a starting dose of 150 IU of recombinant follicular stimulating hormone (FSH) daily. Eight polymorphisms were genotyped. Results: A total of 94 women (mean age 30.71 ± 2.61) were recruited. Fewer fertilized and mature oocytes were retrieved in homozygous carriers of luteinizing hormone/choriogonadotropin receptor (LHCGR) 291 (T/T) than in heterozygous C/T carriers (p = 0.035 and p = 0.05, respectively). In FSH receptor (FSHR) rs6165 and FSHR rs6166 carriers, the ratio between total gonadotropin consumption and number of oocytes retrieved differed significantly among three genotypes (p = 0.050), and the ratio was lower in homozygous A/A carriers than in homozygous G/G and heterozygous carriers. Women who co-expressed allele G in FSHR-29 rs1394205 and FSHR rs6166 and allele C LHCGR 291 rs12470652 are characterized by an increased ratio between total FSH dosage and number of oocytes collected after ovarian stimulation (risk ratio: 5.44, CI 95%: 3.18–7.71, p < 0.001). Conclusions: Our study demonstrated that specific polymorphisms affect the response to ovarian stimulation. Despite this finding, more robust studies are required to establish the clinical utility of genotype analysis before ovarian stimulation.
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spelling pubmed-102986882023-06-28 Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study Alviggi, Carlo Longobardi, Salvatore Papaleo, Enrico Santi, Daniele Alfano, Simona Vanni, Valeria Stella Campitiello, Maria Rosaria De Rosa, Pasquale Strina, Ida Huhtaniemi, Ilpo Pursiheimo, Juha-Pekka D’Hooghe, Thomas Humaidan, Peter Conforti, Alessandro Genes (Basel) Article Background: Specific polymorphisms might influence controlled ovarian stimulation in women undergoing assisted reproductive technologies (ARTs). Data regarding possible interactions of these polymorphisms are still scanty. The aim of this analysis was to evaluate the effect of polymorphisms of gonadotropins and their receptors in women undergoing ART. Methods: A total of 94 normogonadotropic patients from three public ART units were enrolled. Patients underwent a gonadotropin releasing hormone (GnRH) long down-regulation protocol with a starting dose of 150 IU of recombinant follicular stimulating hormone (FSH) daily. Eight polymorphisms were genotyped. Results: A total of 94 women (mean age 30.71 ± 2.61) were recruited. Fewer fertilized and mature oocytes were retrieved in homozygous carriers of luteinizing hormone/choriogonadotropin receptor (LHCGR) 291 (T/T) than in heterozygous C/T carriers (p = 0.035 and p = 0.05, respectively). In FSH receptor (FSHR) rs6165 and FSHR rs6166 carriers, the ratio between total gonadotropin consumption and number of oocytes retrieved differed significantly among three genotypes (p = 0.050), and the ratio was lower in homozygous A/A carriers than in homozygous G/G and heterozygous carriers. Women who co-expressed allele G in FSHR-29 rs1394205 and FSHR rs6166 and allele C LHCGR 291 rs12470652 are characterized by an increased ratio between total FSH dosage and number of oocytes collected after ovarian stimulation (risk ratio: 5.44, CI 95%: 3.18–7.71, p < 0.001). Conclusions: Our study demonstrated that specific polymorphisms affect the response to ovarian stimulation. Despite this finding, more robust studies are required to establish the clinical utility of genotype analysis before ovarian stimulation. MDPI 2023-06-15 /pmc/articles/PMC10298688/ /pubmed/37372449 http://dx.doi.org/10.3390/genes14061269 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alviggi, Carlo
Longobardi, Salvatore
Papaleo, Enrico
Santi, Daniele
Alfano, Simona
Vanni, Valeria Stella
Campitiello, Maria Rosaria
De Rosa, Pasquale
Strina, Ida
Huhtaniemi, Ilpo
Pursiheimo, Juha-Pekka
D’Hooghe, Thomas
Humaidan, Peter
Conforti, Alessandro
Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study
title Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study
title_full Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study
title_fullStr Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study
title_full_unstemmed Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study
title_short Genetic Variants of Gonadotropins and Their Receptors Could Influence Controlled Ovarian Stimulation: IVF Data from a Prospective Multicenter Study
title_sort genetic variants of gonadotropins and their receptors could influence controlled ovarian stimulation: ivf data from a prospective multicenter study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298688/
https://www.ncbi.nlm.nih.gov/pubmed/37372449
http://dx.doi.org/10.3390/genes14061269
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