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Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis

The prevalence of non-alcoholic fatty liver disease (NAFLD) and its severe form, non-alcoholic steatohepatitis (NASH), is higher in men than in women of reproductive age, and postmenopausal women are especially susceptible to developing the disease. Aim: we evaluated if female apolipoprotein E (ApoE...

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Autores principales: Araujo, Layanne C. C., Cruz, Alessandra G., Camargo, Felipe N., Sucupira, Felipe G., Moreira, Gabriela V., Matos, Sandro L., Amaral, Andressa G., Murata, Gilson Masahiro, Carvalho, Carla R. O., Camporez, Joao Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298710/
https://www.ncbi.nlm.nih.gov/pubmed/37372993
http://dx.doi.org/10.3390/ijms24129845
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author Araujo, Layanne C. C.
Cruz, Alessandra G.
Camargo, Felipe N.
Sucupira, Felipe G.
Moreira, Gabriela V.
Matos, Sandro L.
Amaral, Andressa G.
Murata, Gilson Masahiro
Carvalho, Carla R. O.
Camporez, Joao Paulo
author_facet Araujo, Layanne C. C.
Cruz, Alessandra G.
Camargo, Felipe N.
Sucupira, Felipe G.
Moreira, Gabriela V.
Matos, Sandro L.
Amaral, Andressa G.
Murata, Gilson Masahiro
Carvalho, Carla R. O.
Camporez, Joao Paulo
author_sort Araujo, Layanne C. C.
collection PubMed
description The prevalence of non-alcoholic fatty liver disease (NAFLD) and its severe form, non-alcoholic steatohepatitis (NASH), is higher in men than in women of reproductive age, and postmenopausal women are especially susceptible to developing the disease. Aim: we evaluated if female apolipoprotein E (ApoE) KO mice were protected against Western-diet (WD)-induced NASH. Methods: Female ovariectomized (OVX) ApoE KO mice or sham-operated (SHAM) mice were fed either a WD or a regular chow (RC) for 7 weeks. Additionally, OVX mice fed a WD were treated with either estradiol (OVX + E2) or vehicle (OVX). Results: Whole-body fat, plasma glucose, and plasma insulin were increased and associated with increased glucose intolerance in OVX mice fed a WD (OVX + WD). Plasma and hepatic triglycerides, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) hepatic enzymes were also increased in the plasma of OVX + WD group, which was associated with hepatic fibrosis and inflammation. Estradiol replacement in OVX mice reduced body weight, body fat, glycemia, and plasma insulin associated with reduced glucose intolerance. Treatment also reduced hepatic triglycerides, ALT, AST, hepatic fibrosis, and inflammation in OVX mice. Conclusions: These data support the hypothesis that estradiol protects OVX ApoE KO mice from NASH and glucose intolerance.
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spelling pubmed-102987102023-06-28 Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis Araujo, Layanne C. C. Cruz, Alessandra G. Camargo, Felipe N. Sucupira, Felipe G. Moreira, Gabriela V. Matos, Sandro L. Amaral, Andressa G. Murata, Gilson Masahiro Carvalho, Carla R. O. Camporez, Joao Paulo Int J Mol Sci Article The prevalence of non-alcoholic fatty liver disease (NAFLD) and its severe form, non-alcoholic steatohepatitis (NASH), is higher in men than in women of reproductive age, and postmenopausal women are especially susceptible to developing the disease. Aim: we evaluated if female apolipoprotein E (ApoE) KO mice were protected against Western-diet (WD)-induced NASH. Methods: Female ovariectomized (OVX) ApoE KO mice or sham-operated (SHAM) mice were fed either a WD or a regular chow (RC) for 7 weeks. Additionally, OVX mice fed a WD were treated with either estradiol (OVX + E2) or vehicle (OVX). Results: Whole-body fat, plasma glucose, and plasma insulin were increased and associated with increased glucose intolerance in OVX mice fed a WD (OVX + WD). Plasma and hepatic triglycerides, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) hepatic enzymes were also increased in the plasma of OVX + WD group, which was associated with hepatic fibrosis and inflammation. Estradiol replacement in OVX mice reduced body weight, body fat, glycemia, and plasma insulin associated with reduced glucose intolerance. Treatment also reduced hepatic triglycerides, ALT, AST, hepatic fibrosis, and inflammation in OVX mice. Conclusions: These data support the hypothesis that estradiol protects OVX ApoE KO mice from NASH and glucose intolerance. MDPI 2023-06-07 /pmc/articles/PMC10298710/ /pubmed/37372993 http://dx.doi.org/10.3390/ijms24129845 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Araujo, Layanne C. C.
Cruz, Alessandra G.
Camargo, Felipe N.
Sucupira, Felipe G.
Moreira, Gabriela V.
Matos, Sandro L.
Amaral, Andressa G.
Murata, Gilson Masahiro
Carvalho, Carla R. O.
Camporez, Joao Paulo
Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis
title Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis
title_full Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis
title_fullStr Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis
title_full_unstemmed Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis
title_short Estradiol Protects Female ApoE KO Mice against Western-Diet-Induced Non-Alcoholic Steatohepatitis
title_sort estradiol protects female apoe ko mice against western-diet-induced non-alcoholic steatohepatitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298710/
https://www.ncbi.nlm.nih.gov/pubmed/37372993
http://dx.doi.org/10.3390/ijms24129845
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