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First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula
Trichopoda pennipes is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, Nezara viridula, and leaf-footed bug, Leptoglossus phyllopus. To be used successfully as a biological control agent, the fly must selectively parasitize the ta...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298747/ https://www.ncbi.nlm.nih.gov/pubmed/37372352 http://dx.doi.org/10.3390/genes14061172 |
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author | Bogale, Mesfin Mishra, Shova Stacey, Kendall Rooney, Lillie Barreto, Paula Bishop, Gina Bossert, Katherine Bremer, Kalista Bustamante, Daniel Chan, Lila Chau, Quan Cordo, Julian Diaz, Alyssa Hacker, Jordan Hadaegh, Lily Hibshman, Taryn Lastra, Kimberly Lee, Fion Mattia, Alexandra Nguyen, Bao Overton, Gretchen Reis, Victoria Rhodes, Daniel Roeder, Emily Rush, Muhamed Salichs, Oscar Seslija, Mateo Stylianou, Nicholas Vemugunta, Vivek Yun, Min Auletta, Anthony Leppla, Norman DiGennaro, Peter |
author_facet | Bogale, Mesfin Mishra, Shova Stacey, Kendall Rooney, Lillie Barreto, Paula Bishop, Gina Bossert, Katherine Bremer, Kalista Bustamante, Daniel Chan, Lila Chau, Quan Cordo, Julian Diaz, Alyssa Hacker, Jordan Hadaegh, Lily Hibshman, Taryn Lastra, Kimberly Lee, Fion Mattia, Alexandra Nguyen, Bao Overton, Gretchen Reis, Victoria Rhodes, Daniel Roeder, Emily Rush, Muhamed Salichs, Oscar Seslija, Mateo Stylianou, Nicholas Vemugunta, Vivek Yun, Min Auletta, Anthony Leppla, Norman DiGennaro, Peter |
author_sort | Bogale, Mesfin |
collection | PubMed |
description | Trichopoda pennipes is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, Nezara viridula, and leaf-footed bug, Leptoglossus phyllopus. To be used successfully as a biological control agent, the fly must selectively parasitize the target host species. Differences in the host preference of T. pennipes were assessed by assembling the nuclear and mitochondrial genomes of 38 flies reared from field-collected N. viridula and L. phyllopus. High-quality de novo draft genomes of T. pennipes were assembled using long-read sequencing. The assembly totaled 672 MB distributed among 561 contigs, having an N50 of 11.9 MB and a GC of 31.7%, with the longest contig at 28 MB. The genome was assessed for completeness using BUSCO in the Insecta dataset, resulting in a score of 99.4%, and 97.4% of the genes were single copy-loci. The mitochondrial genomes of the 38 T. pennipes flies were sequenced and compared to identify possible host-determined sibling species. The assembled circular genomes ranged from 15,345 bp to 16,390 bp and encode 22 tRNAs, two rRNAs, and 13 protein-coding genes (PCGs). There were no differences in the architecture of these genomes. Phylogenetic analyses using sequence information from 13 PCGs and the two rRNAs individually or as a combined dataset resolved the parasitoids into two distinct lineages: T. pennipes that parasitized both N. viridula and L. phyllopus, and others that parasitized only L. phyllopus. |
format | Online Article Text |
id | pubmed-10298747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102987472023-06-28 First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula Bogale, Mesfin Mishra, Shova Stacey, Kendall Rooney, Lillie Barreto, Paula Bishop, Gina Bossert, Katherine Bremer, Kalista Bustamante, Daniel Chan, Lila Chau, Quan Cordo, Julian Diaz, Alyssa Hacker, Jordan Hadaegh, Lily Hibshman, Taryn Lastra, Kimberly Lee, Fion Mattia, Alexandra Nguyen, Bao Overton, Gretchen Reis, Victoria Rhodes, Daniel Roeder, Emily Rush, Muhamed Salichs, Oscar Seslija, Mateo Stylianou, Nicholas Vemugunta, Vivek Yun, Min Auletta, Anthony Leppla, Norman DiGennaro, Peter Genes (Basel) Article Trichopoda pennipes is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, Nezara viridula, and leaf-footed bug, Leptoglossus phyllopus. To be used successfully as a biological control agent, the fly must selectively parasitize the target host species. Differences in the host preference of T. pennipes were assessed by assembling the nuclear and mitochondrial genomes of 38 flies reared from field-collected N. viridula and L. phyllopus. High-quality de novo draft genomes of T. pennipes were assembled using long-read sequencing. The assembly totaled 672 MB distributed among 561 contigs, having an N50 of 11.9 MB and a GC of 31.7%, with the longest contig at 28 MB. The genome was assessed for completeness using BUSCO in the Insecta dataset, resulting in a score of 99.4%, and 97.4% of the genes were single copy-loci. The mitochondrial genomes of the 38 T. pennipes flies were sequenced and compared to identify possible host-determined sibling species. The assembled circular genomes ranged from 15,345 bp to 16,390 bp and encode 22 tRNAs, two rRNAs, and 13 protein-coding genes (PCGs). There were no differences in the architecture of these genomes. Phylogenetic analyses using sequence information from 13 PCGs and the two rRNAs individually or as a combined dataset resolved the parasitoids into two distinct lineages: T. pennipes that parasitized both N. viridula and L. phyllopus, and others that parasitized only L. phyllopus. MDPI 2023-05-27 /pmc/articles/PMC10298747/ /pubmed/37372352 http://dx.doi.org/10.3390/genes14061172 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bogale, Mesfin Mishra, Shova Stacey, Kendall Rooney, Lillie Barreto, Paula Bishop, Gina Bossert, Katherine Bremer, Kalista Bustamante, Daniel Chan, Lila Chau, Quan Cordo, Julian Diaz, Alyssa Hacker, Jordan Hadaegh, Lily Hibshman, Taryn Lastra, Kimberly Lee, Fion Mattia, Alexandra Nguyen, Bao Overton, Gretchen Reis, Victoria Rhodes, Daniel Roeder, Emily Rush, Muhamed Salichs, Oscar Seslija, Mateo Stylianou, Nicholas Vemugunta, Vivek Yun, Min Auletta, Anthony Leppla, Norman DiGennaro, Peter First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula |
title | First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula |
title_full | First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula |
title_fullStr | First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula |
title_full_unstemmed | First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula |
title_short | First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula |
title_sort | first description of the nuclear and mitochondrial genomes and associated host preference of trichopoda pennipes, a parasitoid of nezara viridula |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298747/ https://www.ncbi.nlm.nih.gov/pubmed/37372352 http://dx.doi.org/10.3390/genes14061172 |
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