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First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula

Trichopoda pennipes is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, Nezara viridula, and leaf-footed bug, Leptoglossus phyllopus. To be used successfully as a biological control agent, the fly must selectively parasitize the ta...

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Autores principales: Bogale, Mesfin, Mishra, Shova, Stacey, Kendall, Rooney, Lillie, Barreto, Paula, Bishop, Gina, Bossert, Katherine, Bremer, Kalista, Bustamante, Daniel, Chan, Lila, Chau, Quan, Cordo, Julian, Diaz, Alyssa, Hacker, Jordan, Hadaegh, Lily, Hibshman, Taryn, Lastra, Kimberly, Lee, Fion, Mattia, Alexandra, Nguyen, Bao, Overton, Gretchen, Reis, Victoria, Rhodes, Daniel, Roeder, Emily, Rush, Muhamed, Salichs, Oscar, Seslija, Mateo, Stylianou, Nicholas, Vemugunta, Vivek, Yun, Min, Auletta, Anthony, Leppla, Norman, DiGennaro, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298747/
https://www.ncbi.nlm.nih.gov/pubmed/37372352
http://dx.doi.org/10.3390/genes14061172
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author Bogale, Mesfin
Mishra, Shova
Stacey, Kendall
Rooney, Lillie
Barreto, Paula
Bishop, Gina
Bossert, Katherine
Bremer, Kalista
Bustamante, Daniel
Chan, Lila
Chau, Quan
Cordo, Julian
Diaz, Alyssa
Hacker, Jordan
Hadaegh, Lily
Hibshman, Taryn
Lastra, Kimberly
Lee, Fion
Mattia, Alexandra
Nguyen, Bao
Overton, Gretchen
Reis, Victoria
Rhodes, Daniel
Roeder, Emily
Rush, Muhamed
Salichs, Oscar
Seslija, Mateo
Stylianou, Nicholas
Vemugunta, Vivek
Yun, Min
Auletta, Anthony
Leppla, Norman
DiGennaro, Peter
author_facet Bogale, Mesfin
Mishra, Shova
Stacey, Kendall
Rooney, Lillie
Barreto, Paula
Bishop, Gina
Bossert, Katherine
Bremer, Kalista
Bustamante, Daniel
Chan, Lila
Chau, Quan
Cordo, Julian
Diaz, Alyssa
Hacker, Jordan
Hadaegh, Lily
Hibshman, Taryn
Lastra, Kimberly
Lee, Fion
Mattia, Alexandra
Nguyen, Bao
Overton, Gretchen
Reis, Victoria
Rhodes, Daniel
Roeder, Emily
Rush, Muhamed
Salichs, Oscar
Seslija, Mateo
Stylianou, Nicholas
Vemugunta, Vivek
Yun, Min
Auletta, Anthony
Leppla, Norman
DiGennaro, Peter
author_sort Bogale, Mesfin
collection PubMed
description Trichopoda pennipes is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, Nezara viridula, and leaf-footed bug, Leptoglossus phyllopus. To be used successfully as a biological control agent, the fly must selectively parasitize the target host species. Differences in the host preference of T. pennipes were assessed by assembling the nuclear and mitochondrial genomes of 38 flies reared from field-collected N. viridula and L. phyllopus. High-quality de novo draft genomes of T. pennipes were assembled using long-read sequencing. The assembly totaled 672 MB distributed among 561 contigs, having an N50 of 11.9 MB and a GC of 31.7%, with the longest contig at 28 MB. The genome was assessed for completeness using BUSCO in the Insecta dataset, resulting in a score of 99.4%, and 97.4% of the genes were single copy-loci. The mitochondrial genomes of the 38 T. pennipes flies were sequenced and compared to identify possible host-determined sibling species. The assembled circular genomes ranged from 15,345 bp to 16,390 bp and encode 22 tRNAs, two rRNAs, and 13 protein-coding genes (PCGs). There were no differences in the architecture of these genomes. Phylogenetic analyses using sequence information from 13 PCGs and the two rRNAs individually or as a combined dataset resolved the parasitoids into two distinct lineages: T. pennipes that parasitized both N. viridula and L. phyllopus, and others that parasitized only L. phyllopus.
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spelling pubmed-102987472023-06-28 First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula Bogale, Mesfin Mishra, Shova Stacey, Kendall Rooney, Lillie Barreto, Paula Bishop, Gina Bossert, Katherine Bremer, Kalista Bustamante, Daniel Chan, Lila Chau, Quan Cordo, Julian Diaz, Alyssa Hacker, Jordan Hadaegh, Lily Hibshman, Taryn Lastra, Kimberly Lee, Fion Mattia, Alexandra Nguyen, Bao Overton, Gretchen Reis, Victoria Rhodes, Daniel Roeder, Emily Rush, Muhamed Salichs, Oscar Seslija, Mateo Stylianou, Nicholas Vemugunta, Vivek Yun, Min Auletta, Anthony Leppla, Norman DiGennaro, Peter Genes (Basel) Article Trichopoda pennipes is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, Nezara viridula, and leaf-footed bug, Leptoglossus phyllopus. To be used successfully as a biological control agent, the fly must selectively parasitize the target host species. Differences in the host preference of T. pennipes were assessed by assembling the nuclear and mitochondrial genomes of 38 flies reared from field-collected N. viridula and L. phyllopus. High-quality de novo draft genomes of T. pennipes were assembled using long-read sequencing. The assembly totaled 672 MB distributed among 561 contigs, having an N50 of 11.9 MB and a GC of 31.7%, with the longest contig at 28 MB. The genome was assessed for completeness using BUSCO in the Insecta dataset, resulting in a score of 99.4%, and 97.4% of the genes were single copy-loci. The mitochondrial genomes of the 38 T. pennipes flies were sequenced and compared to identify possible host-determined sibling species. The assembled circular genomes ranged from 15,345 bp to 16,390 bp and encode 22 tRNAs, two rRNAs, and 13 protein-coding genes (PCGs). There were no differences in the architecture of these genomes. Phylogenetic analyses using sequence information from 13 PCGs and the two rRNAs individually or as a combined dataset resolved the parasitoids into two distinct lineages: T. pennipes that parasitized both N. viridula and L. phyllopus, and others that parasitized only L. phyllopus. MDPI 2023-05-27 /pmc/articles/PMC10298747/ /pubmed/37372352 http://dx.doi.org/10.3390/genes14061172 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bogale, Mesfin
Mishra, Shova
Stacey, Kendall
Rooney, Lillie
Barreto, Paula
Bishop, Gina
Bossert, Katherine
Bremer, Kalista
Bustamante, Daniel
Chan, Lila
Chau, Quan
Cordo, Julian
Diaz, Alyssa
Hacker, Jordan
Hadaegh, Lily
Hibshman, Taryn
Lastra, Kimberly
Lee, Fion
Mattia, Alexandra
Nguyen, Bao
Overton, Gretchen
Reis, Victoria
Rhodes, Daniel
Roeder, Emily
Rush, Muhamed
Salichs, Oscar
Seslija, Mateo
Stylianou, Nicholas
Vemugunta, Vivek
Yun, Min
Auletta, Anthony
Leppla, Norman
DiGennaro, Peter
First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula
title First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula
title_full First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula
title_fullStr First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula
title_full_unstemmed First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula
title_short First Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of Trichopoda pennipes, a Parasitoid of Nezara viridula
title_sort first description of the nuclear and mitochondrial genomes and associated host preference of trichopoda pennipes, a parasitoid of nezara viridula
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298747/
https://www.ncbi.nlm.nih.gov/pubmed/37372352
http://dx.doi.org/10.3390/genes14061172
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