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Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet

This study aimed to investigate the effects of a high-fat and cholesterol diet (HFCD) on rats’ gastric mucosa. In the study, a total of 16 (40-day-old Sprague Dawley) male rats were used and randomly divided into two groups (each consisted of eight rats). The rats in control group had no implementat...

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Autores principales: Gündoğdu, Habibe, Sari, Ebru Karadağ
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298842/
https://www.ncbi.nlm.nih.gov/pubmed/37383650
http://dx.doi.org/10.30466/vrf.2022.551598.3441
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author Gündoğdu, Habibe
Sari, Ebru Karadağ
author_facet Gündoğdu, Habibe
Sari, Ebru Karadağ
author_sort Gündoğdu, Habibe
collection PubMed
description This study aimed to investigate the effects of a high-fat and cholesterol diet (HFCD) on rats’ gastric mucosa. In the study, a total of 16 (40-day-old Sprague Dawley) male rats were used and randomly divided into two groups (each consisted of eight rats). The rats in control group had no implementations other than normal feeding. For 10 weeks, rats in a high-fat with cholesterol diet group had daily energy amounts provided by pellet feed mixed with 65.00% butter and 2.00% cholesterol. Before beginning the study and at the end, rats live weight was recorded and their blood samples were taken for biochemical analyses. Hematoxylin and Eosin and Crossman’s triple staining techniques were used to investigate the general structure of gastric tissue. The rats fed with HFCD had statistically significant increases in live weight and total cholesterol values, and were identified to have gastric tissue degeneration. The rats’ gastric tissue in control group had more intense somatostatin (SST) immunoreactivity in parietal and chief cells than the HFCD group. It was determined that feeding with the HFCD has a negative effect on SST secretion in rats and hence, this may have important areas of use such as in gastric cancer treatment and preventing complications linked to gastric diseases.
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spelling pubmed-102988422023-06-28 Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet Gündoğdu, Habibe Sari, Ebru Karadağ Vet Res Forum Original Article This study aimed to investigate the effects of a high-fat and cholesterol diet (HFCD) on rats’ gastric mucosa. In the study, a total of 16 (40-day-old Sprague Dawley) male rats were used and randomly divided into two groups (each consisted of eight rats). The rats in control group had no implementations other than normal feeding. For 10 weeks, rats in a high-fat with cholesterol diet group had daily energy amounts provided by pellet feed mixed with 65.00% butter and 2.00% cholesterol. Before beginning the study and at the end, rats live weight was recorded and their blood samples were taken for biochemical analyses. Hematoxylin and Eosin and Crossman’s triple staining techniques were used to investigate the general structure of gastric tissue. The rats fed with HFCD had statistically significant increases in live weight and total cholesterol values, and were identified to have gastric tissue degeneration. The rats’ gastric tissue in control group had more intense somatostatin (SST) immunoreactivity in parietal and chief cells than the HFCD group. It was determined that feeding with the HFCD has a negative effect on SST secretion in rats and hence, this may have important areas of use such as in gastric cancer treatment and preventing complications linked to gastric diseases. Urmia University Press 2023 2023-06-15 /pmc/articles/PMC10298842/ /pubmed/37383650 http://dx.doi.org/10.30466/vrf.2022.551598.3441 Text en https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.https://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Gündoğdu, Habibe
Sari, Ebru Karadağ
Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
title Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
title_full Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
title_fullStr Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
title_full_unstemmed Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
title_short Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
title_sort immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298842/
https://www.ncbi.nlm.nih.gov/pubmed/37383650
http://dx.doi.org/10.30466/vrf.2022.551598.3441
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