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Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease
Blister blight (BB) disease is caused by the obligate biotrophic fungal pathogen Exobasidium vexans Massee and seriously affects the yield and quality of Camellia sinensis. The use of chemical pesticides on tea leaves substantially increases the toxic risks of tea consumption. Botanic fungicide isob...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298858/ https://www.ncbi.nlm.nih.gov/pubmed/37373374 http://dx.doi.org/10.3390/ijms241210225 |
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author | Yang, Xiuju Cao, Kunqian Ren, Xiaoli Cao, Guangyun Xun, Weizhi Qin, Jiayong Zhou, Xia Jin, Linhong |
author_facet | Yang, Xiuju Cao, Kunqian Ren, Xiaoli Cao, Guangyun Xun, Weizhi Qin, Jiayong Zhou, Xia Jin, Linhong |
author_sort | Yang, Xiuju |
collection | PubMed |
description | Blister blight (BB) disease is caused by the obligate biotrophic fungal pathogen Exobasidium vexans Massee and seriously affects the yield and quality of Camellia sinensis. The use of chemical pesticides on tea leaves substantially increases the toxic risks of tea consumption. Botanic fungicide isobavachalcone (IBC) has the potential to control fungal diseases on many crops but has not been used on tea plants. In this study, the field control effects of IBC were evaluated by comparison and in combination with natural elicitor chitosan oligosaccharides (COSs) and the chemical pesticide pyraclostrobin (Py), and the preliminary action mode of IBC was also investigated. The bioassay results for IBC or its combination with COSs showed a remarkable control effect against BB (61.72% and 70.46%). IBC, like COSs, could improve the disease resistance of tea plants by enhancing the activity of tea-plant-related defense enzymes, including polyphenol oxidase (PPO), catalase (CAT), phenylalanine aminolase (PAL), peroxidase (POD), superoxide dismutase (SOD), β-1,3-glucanase (Glu), and chitinase enzymes. The fungal community structure and diversity of the diseased tea leaves were examined using Illumina MiSeq sequencing of the internal transcribed spacer (ITS) region of the ribosomal rDNA genes. It was obvious that IBC could significantly alter the species’ richness and the diversity of the fungal community in affected plant sites. This study broadens the application range of IBC and provides an important strategy for the control of BB disease. |
format | Online Article Text |
id | pubmed-10298858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102988582023-06-28 Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease Yang, Xiuju Cao, Kunqian Ren, Xiaoli Cao, Guangyun Xun, Weizhi Qin, Jiayong Zhou, Xia Jin, Linhong Int J Mol Sci Article Blister blight (BB) disease is caused by the obligate biotrophic fungal pathogen Exobasidium vexans Massee and seriously affects the yield and quality of Camellia sinensis. The use of chemical pesticides on tea leaves substantially increases the toxic risks of tea consumption. Botanic fungicide isobavachalcone (IBC) has the potential to control fungal diseases on many crops but has not been used on tea plants. In this study, the field control effects of IBC were evaluated by comparison and in combination with natural elicitor chitosan oligosaccharides (COSs) and the chemical pesticide pyraclostrobin (Py), and the preliminary action mode of IBC was also investigated. The bioassay results for IBC or its combination with COSs showed a remarkable control effect against BB (61.72% and 70.46%). IBC, like COSs, could improve the disease resistance of tea plants by enhancing the activity of tea-plant-related defense enzymes, including polyphenol oxidase (PPO), catalase (CAT), phenylalanine aminolase (PAL), peroxidase (POD), superoxide dismutase (SOD), β-1,3-glucanase (Glu), and chitinase enzymes. The fungal community structure and diversity of the diseased tea leaves were examined using Illumina MiSeq sequencing of the internal transcribed spacer (ITS) region of the ribosomal rDNA genes. It was obvious that IBC could significantly alter the species’ richness and the diversity of the fungal community in affected plant sites. This study broadens the application range of IBC and provides an important strategy for the control of BB disease. MDPI 2023-06-16 /pmc/articles/PMC10298858/ /pubmed/37373374 http://dx.doi.org/10.3390/ijms241210225 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Xiuju Cao, Kunqian Ren, Xiaoli Cao, Guangyun Xun, Weizhi Qin, Jiayong Zhou, Xia Jin, Linhong Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
title | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
title_full | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
title_fullStr | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
title_full_unstemmed | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
title_short | Field Control Effect and Initial Mechanism: A Study of Isobavachalcone against Blister Blight Disease |
title_sort | field control effect and initial mechanism: a study of isobavachalcone against blister blight disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298858/ https://www.ncbi.nlm.nih.gov/pubmed/37373374 http://dx.doi.org/10.3390/ijms241210225 |
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