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Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes

Gold nanoparticles (AuNPs) are promising candidates in various biomedical applications such as sensors, imaging, and cancer therapy. Understanding the influence of AuNPs on lipid membranes is important to assure their safety in the biological environment and to improve their scope in nanomedicine. I...

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Autores principales: Santhosh, Poornima Budime, Tenev, Tihomir, Šturm, Luka, Ulrih, Nataša Poklar, Genova, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298864/
https://www.ncbi.nlm.nih.gov/pubmed/37373371
http://dx.doi.org/10.3390/ijms241210226
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author Santhosh, Poornima Budime
Tenev, Tihomir
Šturm, Luka
Ulrih, Nataša Poklar
Genova, Julia
author_facet Santhosh, Poornima Budime
Tenev, Tihomir
Šturm, Luka
Ulrih, Nataša Poklar
Genova, Julia
author_sort Santhosh, Poornima Budime
collection PubMed
description Gold nanoparticles (AuNPs) are promising candidates in various biomedical applications such as sensors, imaging, and cancer therapy. Understanding the influence of AuNPs on lipid membranes is important to assure their safety in the biological environment and to improve their scope in nanomedicine. In this regard, the present study aimed to analyze the effects of different concentrations (0.5, 1, and 2 wt.%) of dodecanethiol functionalized hydrophobic AuNPs on the structure and fluidity of zwitterionic 1-stearoyl-2-oleoyl-sn-glycerol-3-phosphocholine (SOPC) lipid bilayer membranes using Fourier-transform infrared (FTIR) spectroscopy and fluorescent spectroscopy. The size of AuNPs was found to be 2.2 ± 1.1 nm using transmission electron microscopy. FTIR results have shown that the AuNPs induced a slight shift in methylene stretching bands, while the band positions of carbonyl and phosphate group stretching were unaffected. Temperature-dependent fluorescent anisotropy measurements showed that the incorporation of AuNPs up to 2 wt.% did not affect the lipid order in membranes. Overall, these results indicate that the hydrophobic AuNPs in the studied concentration did not cause any significant alterations in the structure and membrane fluidity, which suggests the suitability of these particles to form liposome–AuNP hybrids for diverse biomedical applications including drug delivery and therapy.
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spelling pubmed-102988642023-06-28 Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes Santhosh, Poornima Budime Tenev, Tihomir Šturm, Luka Ulrih, Nataša Poklar Genova, Julia Int J Mol Sci Article Gold nanoparticles (AuNPs) are promising candidates in various biomedical applications such as sensors, imaging, and cancer therapy. Understanding the influence of AuNPs on lipid membranes is important to assure their safety in the biological environment and to improve their scope in nanomedicine. In this regard, the present study aimed to analyze the effects of different concentrations (0.5, 1, and 2 wt.%) of dodecanethiol functionalized hydrophobic AuNPs on the structure and fluidity of zwitterionic 1-stearoyl-2-oleoyl-sn-glycerol-3-phosphocholine (SOPC) lipid bilayer membranes using Fourier-transform infrared (FTIR) spectroscopy and fluorescent spectroscopy. The size of AuNPs was found to be 2.2 ± 1.1 nm using transmission electron microscopy. FTIR results have shown that the AuNPs induced a slight shift in methylene stretching bands, while the band positions of carbonyl and phosphate group stretching were unaffected. Temperature-dependent fluorescent anisotropy measurements showed that the incorporation of AuNPs up to 2 wt.% did not affect the lipid order in membranes. Overall, these results indicate that the hydrophobic AuNPs in the studied concentration did not cause any significant alterations in the structure and membrane fluidity, which suggests the suitability of these particles to form liposome–AuNP hybrids for diverse biomedical applications including drug delivery and therapy. MDPI 2023-06-16 /pmc/articles/PMC10298864/ /pubmed/37373371 http://dx.doi.org/10.3390/ijms241210226 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santhosh, Poornima Budime
Tenev, Tihomir
Šturm, Luka
Ulrih, Nataša Poklar
Genova, Julia
Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes
title Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes
title_full Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes
title_fullStr Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes
title_full_unstemmed Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes
title_short Effects of Hydrophobic Gold Nanoparticles on Structure and Fluidity of SOPC Lipid Membranes
title_sort effects of hydrophobic gold nanoparticles on structure and fluidity of sopc lipid membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298864/
https://www.ncbi.nlm.nih.gov/pubmed/37373371
http://dx.doi.org/10.3390/ijms241210226
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