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Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar
Leaf blight is a fungal disease that mainly affects the growth and development of leaves in plants. To investigate the molecular mechanisms of leaf blight defense in poplar, we performed RNA-Seq and enzyme activity assays on the Populus simonii × Populus nigra leaves inoculated with Alternaria alter...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298882/ https://www.ncbi.nlm.nih.gov/pubmed/37373194 http://dx.doi.org/10.3390/ijms241210047 |
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author | Wang, Ruiqi Wang, Yuting Yao, Wenjing Ge, Wengong Jiang, Tingbo Zhou, Boru |
author_facet | Wang, Ruiqi Wang, Yuting Yao, Wenjing Ge, Wengong Jiang, Tingbo Zhou, Boru |
author_sort | Wang, Ruiqi |
collection | PubMed |
description | Leaf blight is a fungal disease that mainly affects the growth and development of leaves in plants. To investigate the molecular mechanisms of leaf blight defense in poplar, we performed RNA-Seq and enzyme activity assays on the Populus simonii × Populus nigra leaves inoculated with Alternaria alternate fungus. Through weighted gene co-expression network analysis (WGCNA), we obtained co-expression gene modules significantly associated with SOD and POD activities, containing 183 and 275 genes, respectively. We then constructed a co-expression network of poplar genes related to leaf blight resistance based on weight values. Additionally, we identified hub transcription factors (TFs) and structural genes in the network. The network was dominated by 15 TFs, and four out of them, including ATWRKY75, ANAC062, ATMYB23 and ATEBP, had high connectivity in the network, which might play important functions in leaf blight defense. In addition, GO enrichment analysis revealed a total of 44 structural genes involved in biotic stress, resistance, cell wall and immune-related biological processes in the network. Among them, there were 16 highly linked structural genes in the central part, which may be directly involved in poplar resistance to leaf blight. The study explores key genes associated with leaf blight defense in poplar, which further gains an understanding of the molecular mechanisms of biotic stress response in plants. |
format | Online Article Text |
id | pubmed-10298882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102988822023-06-28 Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar Wang, Ruiqi Wang, Yuting Yao, Wenjing Ge, Wengong Jiang, Tingbo Zhou, Boru Int J Mol Sci Article Leaf blight is a fungal disease that mainly affects the growth and development of leaves in plants. To investigate the molecular mechanisms of leaf blight defense in poplar, we performed RNA-Seq and enzyme activity assays on the Populus simonii × Populus nigra leaves inoculated with Alternaria alternate fungus. Through weighted gene co-expression network analysis (WGCNA), we obtained co-expression gene modules significantly associated with SOD and POD activities, containing 183 and 275 genes, respectively. We then constructed a co-expression network of poplar genes related to leaf blight resistance based on weight values. Additionally, we identified hub transcription factors (TFs) and structural genes in the network. The network was dominated by 15 TFs, and four out of them, including ATWRKY75, ANAC062, ATMYB23 and ATEBP, had high connectivity in the network, which might play important functions in leaf blight defense. In addition, GO enrichment analysis revealed a total of 44 structural genes involved in biotic stress, resistance, cell wall and immune-related biological processes in the network. Among them, there were 16 highly linked structural genes in the central part, which may be directly involved in poplar resistance to leaf blight. The study explores key genes associated with leaf blight defense in poplar, which further gains an understanding of the molecular mechanisms of biotic stress response in plants. MDPI 2023-06-12 /pmc/articles/PMC10298882/ /pubmed/37373194 http://dx.doi.org/10.3390/ijms241210047 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Ruiqi Wang, Yuting Yao, Wenjing Ge, Wengong Jiang, Tingbo Zhou, Boru Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar |
title | Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar |
title_full | Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar |
title_fullStr | Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar |
title_full_unstemmed | Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar |
title_short | Transcriptome Sequencing and WGCNA Reveal Key Genes in Response to Leaf Blight in Poplar |
title_sort | transcriptome sequencing and wgcna reveal key genes in response to leaf blight in poplar |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298882/ https://www.ncbi.nlm.nih.gov/pubmed/37373194 http://dx.doi.org/10.3390/ijms241210047 |
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