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Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice

Anxiety and depressive disorders are closely associated; however, the pathophysiology of these disorders remains poorly understood. Further exploration of the mechanisms involved in anxiety and depression such as the stress response may provide new knowledge that will contribute to our understanding...

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Autores principales: Herselman, Mauritz Frederick, Lin, Liying, Luo, Shayan, Yamanaka, Akihiro, Zhou, Xin-Fu, Bobrovskaya, Larisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298965/
https://www.ncbi.nlm.nih.gov/pubmed/37373499
http://dx.doi.org/10.3390/ijms241210353
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author Herselman, Mauritz Frederick
Lin, Liying
Luo, Shayan
Yamanaka, Akihiro
Zhou, Xin-Fu
Bobrovskaya, Larisa
author_facet Herselman, Mauritz Frederick
Lin, Liying
Luo, Shayan
Yamanaka, Akihiro
Zhou, Xin-Fu
Bobrovskaya, Larisa
author_sort Herselman, Mauritz Frederick
collection PubMed
description Anxiety and depressive disorders are closely associated; however, the pathophysiology of these disorders remains poorly understood. Further exploration of the mechanisms involved in anxiety and depression such as the stress response may provide new knowledge that will contribute to our understanding of these disorders. Fifty-eight 8–12-week-old C57BL6 mice were separated into experimental groups by sex as follows: male controls (n = 14), male restraint stress (n = 14), female controls (n = 15) and female restraint stress (n = 15). These mice were taken through a 4-week randomised chronic restraint stress protocol, and their behaviour, as well as tryptophan metabolism and synaptic proteins, were measured in the prefrontal cortex and hippocampus. Adrenal catecholamine regulation was also measured. The female mice showed greater anxiety-like behaviour than their male counterparts. Tryptophan metabolism was unaffected by stress, but some basal sex characteristics were noted. Synaptic proteins were reduced in the hippocampus in stressed females but increased in the prefrontal cortex of all female mice. These changes were not found in any males. Finally, the stressed female mice showed increased catecholamine biosynthesis capability, but this effect was not found in males. Future studies in animal models should consider these sex differences when evaluating mechanisms related to chronic stress and depression.
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spelling pubmed-102989652023-06-28 Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice Herselman, Mauritz Frederick Lin, Liying Luo, Shayan Yamanaka, Akihiro Zhou, Xin-Fu Bobrovskaya, Larisa Int J Mol Sci Article Anxiety and depressive disorders are closely associated; however, the pathophysiology of these disorders remains poorly understood. Further exploration of the mechanisms involved in anxiety and depression such as the stress response may provide new knowledge that will contribute to our understanding of these disorders. Fifty-eight 8–12-week-old C57BL6 mice were separated into experimental groups by sex as follows: male controls (n = 14), male restraint stress (n = 14), female controls (n = 15) and female restraint stress (n = 15). These mice were taken through a 4-week randomised chronic restraint stress protocol, and their behaviour, as well as tryptophan metabolism and synaptic proteins, were measured in the prefrontal cortex and hippocampus. Adrenal catecholamine regulation was also measured. The female mice showed greater anxiety-like behaviour than their male counterparts. Tryptophan metabolism was unaffected by stress, but some basal sex characteristics were noted. Synaptic proteins were reduced in the hippocampus in stressed females but increased in the prefrontal cortex of all female mice. These changes were not found in any males. Finally, the stressed female mice showed increased catecholamine biosynthesis capability, but this effect was not found in males. Future studies in animal models should consider these sex differences when evaluating mechanisms related to chronic stress and depression. MDPI 2023-06-19 /pmc/articles/PMC10298965/ /pubmed/37373499 http://dx.doi.org/10.3390/ijms241210353 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Herselman, Mauritz Frederick
Lin, Liying
Luo, Shayan
Yamanaka, Akihiro
Zhou, Xin-Fu
Bobrovskaya, Larisa
Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice
title Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice
title_full Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice
title_fullStr Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice
title_full_unstemmed Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice
title_short Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice
title_sort sex-dependent effects of chronic restraint stress on mood-related behaviours and neurochemistry in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10298965/
https://www.ncbi.nlm.nih.gov/pubmed/37373499
http://dx.doi.org/10.3390/ijms241210353
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