Cargando…

Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza

Salvia miltiorrhiza Bunge is an important traditional herb. Salvia miltiorrhiza is distributed in the Sichuan province of China (here called SC). Under natural conditions, it does not bear seeds and its sterility mechanism is still unclear. Through artificial cross, there was defective pistil and pa...

Descripción completa

Detalles Bibliográficos
Autores principales: Liao, Jinqiu, Zhang, Zhizhou, Shang, Yukun, Jiang, Yuanyuan, Su, Zixuan, Deng, Xuexue, Pu, Xiang, Yang, Ruiwu, Zhang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299234/
https://www.ncbi.nlm.nih.gov/pubmed/37373407
http://dx.doi.org/10.3390/ijms241210259
_version_ 1785064313598246912
author Liao, Jinqiu
Zhang, Zhizhou
Shang, Yukun
Jiang, Yuanyuan
Su, Zixuan
Deng, Xuexue
Pu, Xiang
Yang, Ruiwu
Zhang, Li
author_facet Liao, Jinqiu
Zhang, Zhizhou
Shang, Yukun
Jiang, Yuanyuan
Su, Zixuan
Deng, Xuexue
Pu, Xiang
Yang, Ruiwu
Zhang, Li
author_sort Liao, Jinqiu
collection PubMed
description Salvia miltiorrhiza Bunge is an important traditional herb. Salvia miltiorrhiza is distributed in the Sichuan province of China (here called SC). Under natural conditions, it does not bear seeds and its sterility mechanism is still unclear. Through artificial cross, there was defective pistil and partial pollen abortion in these plants. Electron microscopy results showed that the defective pollen wall was caused by delayed degradation of the tapetum. Due to the lack of starch and organelle, the abortive pollen grains showed shrinkage. RNA-seq was performed to explore the molecular mechanisms of pollen abortion. KEGG enrichment analysis suggested that the pathways of phytohormone, starch, lipid, pectin, and phenylpropanoid affected the fertility of S. miltiorrhiza. Moreover, some differentially expressed genes involved in starch synthesis and plant hormone signaling were identified. These results contribute to the molecular mechanism of pollen sterility and provide a more theoretical foundation for molecular-assisted breeding.
format Online
Article
Text
id pubmed-10299234
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102992342023-06-28 Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza Liao, Jinqiu Zhang, Zhizhou Shang, Yukun Jiang, Yuanyuan Su, Zixuan Deng, Xuexue Pu, Xiang Yang, Ruiwu Zhang, Li Int J Mol Sci Article Salvia miltiorrhiza Bunge is an important traditional herb. Salvia miltiorrhiza is distributed in the Sichuan province of China (here called SC). Under natural conditions, it does not bear seeds and its sterility mechanism is still unclear. Through artificial cross, there was defective pistil and partial pollen abortion in these plants. Electron microscopy results showed that the defective pollen wall was caused by delayed degradation of the tapetum. Due to the lack of starch and organelle, the abortive pollen grains showed shrinkage. RNA-seq was performed to explore the molecular mechanisms of pollen abortion. KEGG enrichment analysis suggested that the pathways of phytohormone, starch, lipid, pectin, and phenylpropanoid affected the fertility of S. miltiorrhiza. Moreover, some differentially expressed genes involved in starch synthesis and plant hormone signaling were identified. These results contribute to the molecular mechanism of pollen sterility and provide a more theoretical foundation for molecular-assisted breeding. MDPI 2023-06-17 /pmc/articles/PMC10299234/ /pubmed/37373407 http://dx.doi.org/10.3390/ijms241210259 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liao, Jinqiu
Zhang, Zhizhou
Shang, Yukun
Jiang, Yuanyuan
Su, Zixuan
Deng, Xuexue
Pu, Xiang
Yang, Ruiwu
Zhang, Li
Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza
title Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza
title_full Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza
title_fullStr Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza
title_full_unstemmed Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza
title_short Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza
title_sort anatomy and comparative transcriptome reveal the mechanism of male sterility in salvia miltiorrhiza
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299234/
https://www.ncbi.nlm.nih.gov/pubmed/37373407
http://dx.doi.org/10.3390/ijms241210259
work_keys_str_mv AT liaojinqiu anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT zhangzhizhou anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT shangyukun anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT jiangyuanyuan anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT suzixuan anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT dengxuexue anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT puxiang anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT yangruiwu anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza
AT zhangli anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza