Cargando…
Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza
Salvia miltiorrhiza Bunge is an important traditional herb. Salvia miltiorrhiza is distributed in the Sichuan province of China (here called SC). Under natural conditions, it does not bear seeds and its sterility mechanism is still unclear. Through artificial cross, there was defective pistil and pa...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299234/ https://www.ncbi.nlm.nih.gov/pubmed/37373407 http://dx.doi.org/10.3390/ijms241210259 |
_version_ | 1785064313598246912 |
---|---|
author | Liao, Jinqiu Zhang, Zhizhou Shang, Yukun Jiang, Yuanyuan Su, Zixuan Deng, Xuexue Pu, Xiang Yang, Ruiwu Zhang, Li |
author_facet | Liao, Jinqiu Zhang, Zhizhou Shang, Yukun Jiang, Yuanyuan Su, Zixuan Deng, Xuexue Pu, Xiang Yang, Ruiwu Zhang, Li |
author_sort | Liao, Jinqiu |
collection | PubMed |
description | Salvia miltiorrhiza Bunge is an important traditional herb. Salvia miltiorrhiza is distributed in the Sichuan province of China (here called SC). Under natural conditions, it does not bear seeds and its sterility mechanism is still unclear. Through artificial cross, there was defective pistil and partial pollen abortion in these plants. Electron microscopy results showed that the defective pollen wall was caused by delayed degradation of the tapetum. Due to the lack of starch and organelle, the abortive pollen grains showed shrinkage. RNA-seq was performed to explore the molecular mechanisms of pollen abortion. KEGG enrichment analysis suggested that the pathways of phytohormone, starch, lipid, pectin, and phenylpropanoid affected the fertility of S. miltiorrhiza. Moreover, some differentially expressed genes involved in starch synthesis and plant hormone signaling were identified. These results contribute to the molecular mechanism of pollen sterility and provide a more theoretical foundation for molecular-assisted breeding. |
format | Online Article Text |
id | pubmed-10299234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102992342023-06-28 Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza Liao, Jinqiu Zhang, Zhizhou Shang, Yukun Jiang, Yuanyuan Su, Zixuan Deng, Xuexue Pu, Xiang Yang, Ruiwu Zhang, Li Int J Mol Sci Article Salvia miltiorrhiza Bunge is an important traditional herb. Salvia miltiorrhiza is distributed in the Sichuan province of China (here called SC). Under natural conditions, it does not bear seeds and its sterility mechanism is still unclear. Through artificial cross, there was defective pistil and partial pollen abortion in these plants. Electron microscopy results showed that the defective pollen wall was caused by delayed degradation of the tapetum. Due to the lack of starch and organelle, the abortive pollen grains showed shrinkage. RNA-seq was performed to explore the molecular mechanisms of pollen abortion. KEGG enrichment analysis suggested that the pathways of phytohormone, starch, lipid, pectin, and phenylpropanoid affected the fertility of S. miltiorrhiza. Moreover, some differentially expressed genes involved in starch synthesis and plant hormone signaling were identified. These results contribute to the molecular mechanism of pollen sterility and provide a more theoretical foundation for molecular-assisted breeding. MDPI 2023-06-17 /pmc/articles/PMC10299234/ /pubmed/37373407 http://dx.doi.org/10.3390/ijms241210259 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liao, Jinqiu Zhang, Zhizhou Shang, Yukun Jiang, Yuanyuan Su, Zixuan Deng, Xuexue Pu, Xiang Yang, Ruiwu Zhang, Li Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza |
title | Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza |
title_full | Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza |
title_fullStr | Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza |
title_full_unstemmed | Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza |
title_short | Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza |
title_sort | anatomy and comparative transcriptome reveal the mechanism of male sterility in salvia miltiorrhiza |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299234/ https://www.ncbi.nlm.nih.gov/pubmed/37373407 http://dx.doi.org/10.3390/ijms241210259 |
work_keys_str_mv | AT liaojinqiu anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT zhangzhizhou anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT shangyukun anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT jiangyuanyuan anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT suzixuan anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT dengxuexue anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT puxiang anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT yangruiwu anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza AT zhangli anatomyandcomparativetranscriptomerevealthemechanismofmalesterilityinsalviamiltiorrhiza |