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Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery
Bone defects and infections pose significant challenges for treatment, requiring a comprehensive approach for prevention and treatment. Thus, this study sought to evaluate the efficacy of various bone allografts in the absorption and release of antibiotics. A specially designed high-absorbency, high...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299409/ https://www.ncbi.nlm.nih.gov/pubmed/37367268 http://dx.doi.org/10.3390/jfb14060305 |
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author | Bormann, Nicole Schmock, Aysha Hanke, Anja Eras, Volker Ahmed, Norus Kissner, Maya S. Wildemann, Britt Brune, Jan C. |
author_facet | Bormann, Nicole Schmock, Aysha Hanke, Anja Eras, Volker Ahmed, Norus Kissner, Maya S. Wildemann, Britt Brune, Jan C. |
author_sort | Bormann, Nicole |
collection | PubMed |
description | Bone defects and infections pose significant challenges for treatment, requiring a comprehensive approach for prevention and treatment. Thus, this study sought to evaluate the efficacy of various bone allografts in the absorption and release of antibiotics. A specially designed high-absorbency, high-surface-area carrier graft composed of human demineralized cortical fibers and granulated cancellous bone (fibrous graft) was compared to different human bone allograft types. The groups tested here were three fibrous grafts with rehydration rates of 2.7, 4, and 8 mL/g (F(2.7), F(4), and F(8)); demineralized bone matrix (DBM); cortical granules; mineralized cancellous bone; and demineralized cancellous bone. The absorption capacity of the bone grafts was assessed after rehydration, the duration of absorption varied from 5 to 30 min, and the elution kinetics of gentamicin were determined over 21 days. Furthermore, antimicrobial activity was assessed using a zone of inhibition (ZOI) test with S. aureus. The fibrous grafts exhibited the greatest tissue matrix absorption capacity, while the mineralized cancellous bone revealed the lowest matrix-bound absorption capacity. For F(2.7) and F(4), a greater elution of gentamicin was observed from 4 h and continuously over the first 3 days when compared to the other grafts. Release kinetics were only marginally affected by the varied incubation times. The enhanced absorption capacity of the fibrous grafts resulted in a prolonged antibiotic release and activity. Therefore, fibrous grafts can serve as suitable carrier grafts, as they are able to retain fluids such as antibiotics at their intended destinations, are easy to handle, and allow for a prolonged antibiotic release. Application of these fibrous grafts can enable surgeons to provide longer courses of antibiotic administration for septic orthopedic indications, thus minimizing infections. |
format | Online Article Text |
id | pubmed-10299409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102994092023-06-28 Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery Bormann, Nicole Schmock, Aysha Hanke, Anja Eras, Volker Ahmed, Norus Kissner, Maya S. Wildemann, Britt Brune, Jan C. J Funct Biomater Article Bone defects and infections pose significant challenges for treatment, requiring a comprehensive approach for prevention and treatment. Thus, this study sought to evaluate the efficacy of various bone allografts in the absorption and release of antibiotics. A specially designed high-absorbency, high-surface-area carrier graft composed of human demineralized cortical fibers and granulated cancellous bone (fibrous graft) was compared to different human bone allograft types. The groups tested here were three fibrous grafts with rehydration rates of 2.7, 4, and 8 mL/g (F(2.7), F(4), and F(8)); demineralized bone matrix (DBM); cortical granules; mineralized cancellous bone; and demineralized cancellous bone. The absorption capacity of the bone grafts was assessed after rehydration, the duration of absorption varied from 5 to 30 min, and the elution kinetics of gentamicin were determined over 21 days. Furthermore, antimicrobial activity was assessed using a zone of inhibition (ZOI) test with S. aureus. The fibrous grafts exhibited the greatest tissue matrix absorption capacity, while the mineralized cancellous bone revealed the lowest matrix-bound absorption capacity. For F(2.7) and F(4), a greater elution of gentamicin was observed from 4 h and continuously over the first 3 days when compared to the other grafts. Release kinetics were only marginally affected by the varied incubation times. The enhanced absorption capacity of the fibrous grafts resulted in a prolonged antibiotic release and activity. Therefore, fibrous grafts can serve as suitable carrier grafts, as they are able to retain fluids such as antibiotics at their intended destinations, are easy to handle, and allow for a prolonged antibiotic release. Application of these fibrous grafts can enable surgeons to provide longer courses of antibiotic administration for septic orthopedic indications, thus minimizing infections. MDPI 2023-06-01 /pmc/articles/PMC10299409/ /pubmed/37367268 http://dx.doi.org/10.3390/jfb14060305 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bormann, Nicole Schmock, Aysha Hanke, Anja Eras, Volker Ahmed, Norus Kissner, Maya S. Wildemann, Britt Brune, Jan C. Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery |
title | Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery |
title_full | Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery |
title_fullStr | Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery |
title_full_unstemmed | Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery |
title_short | Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery |
title_sort | analysis of the ability of different allografts to act as carrier grafts for local drug delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299409/ https://www.ncbi.nlm.nih.gov/pubmed/37367268 http://dx.doi.org/10.3390/jfb14060305 |
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