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Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers

Heat stress (HS) can inhibit the growth performance of broilers and cause substantial economic losses. Alterations in bile acid (BA) pools have been reported to be correlated with chronic HS, yet the specific mechanism and whether it is related to gut microbiota remains unclear. In this study, 40 Ru...

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Autores principales: Zhang, Yuting, Chen, Huimin, Cong, Wei, Zhang, Ke, Jia, Yimin, Wu, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299590/
https://www.ncbi.nlm.nih.gov/pubmed/37373380
http://dx.doi.org/10.3390/ijms241210238
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author Zhang, Yuting
Chen, Huimin
Cong, Wei
Zhang, Ke
Jia, Yimin
Wu, Lei
author_facet Zhang, Yuting
Chen, Huimin
Cong, Wei
Zhang, Ke
Jia, Yimin
Wu, Lei
author_sort Zhang, Yuting
collection PubMed
description Heat stress (HS) can inhibit the growth performance of broilers and cause substantial economic losses. Alterations in bile acid (BA) pools have been reported to be correlated with chronic HS, yet the specific mechanism and whether it is related to gut microbiota remains unclear. In this study, 40 Rugao Yellow chickens were randomly selected and distributed into two groups (20 broilers in each group) when reaching 56-day age: a chronic heat stress group (HS, 36 ± 1 °C for 8 h per day in the first 7 days and 36 ± 1 °C for 24 h in the last 7 days) and a control group (CN, 24 ± 1 °C for 24 h within 14 days). Compared with the CN group, total BAs’ serum content decreased, while cholic acid (CA), chenodeoxycholic acid (CDCA), and taurolithocholic acid (TLCA) increased significantly in HS broilers. Moreover, 12α-hydroxylase (CYP8B1) and bile salt export protein (BSEP) were upregulated in the liver, and the expression of fibroblast growth factor 19 (FGF19) decreased in the ileum of HS broilers. There were also significant changes in gut microbial composition, and the enrichment of Peptoniphilus was positively correlated with the increased serum level of TLCA. These results indicate that chronic HS disrupts the homeostasis of BA metabolism in broilers, which is associated with alterations in gut microbiota.
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spelling pubmed-102995902023-06-28 Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers Zhang, Yuting Chen, Huimin Cong, Wei Zhang, Ke Jia, Yimin Wu, Lei Int J Mol Sci Article Heat stress (HS) can inhibit the growth performance of broilers and cause substantial economic losses. Alterations in bile acid (BA) pools have been reported to be correlated with chronic HS, yet the specific mechanism and whether it is related to gut microbiota remains unclear. In this study, 40 Rugao Yellow chickens were randomly selected and distributed into two groups (20 broilers in each group) when reaching 56-day age: a chronic heat stress group (HS, 36 ± 1 °C for 8 h per day in the first 7 days and 36 ± 1 °C for 24 h in the last 7 days) and a control group (CN, 24 ± 1 °C for 24 h within 14 days). Compared with the CN group, total BAs’ serum content decreased, while cholic acid (CA), chenodeoxycholic acid (CDCA), and taurolithocholic acid (TLCA) increased significantly in HS broilers. Moreover, 12α-hydroxylase (CYP8B1) and bile salt export protein (BSEP) were upregulated in the liver, and the expression of fibroblast growth factor 19 (FGF19) decreased in the ileum of HS broilers. There were also significant changes in gut microbial composition, and the enrichment of Peptoniphilus was positively correlated with the increased serum level of TLCA. These results indicate that chronic HS disrupts the homeostasis of BA metabolism in broilers, which is associated with alterations in gut microbiota. MDPI 2023-06-16 /pmc/articles/PMC10299590/ /pubmed/37373380 http://dx.doi.org/10.3390/ijms241210238 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yuting
Chen, Huimin
Cong, Wei
Zhang, Ke
Jia, Yimin
Wu, Lei
Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers
title Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers
title_full Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers
title_fullStr Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers
title_full_unstemmed Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers
title_short Chronic Heat Stress Affects Bile Acid Profile and Gut Microbiota in Broilers
title_sort chronic heat stress affects bile acid profile and gut microbiota in broilers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299590/
https://www.ncbi.nlm.nih.gov/pubmed/37373380
http://dx.doi.org/10.3390/ijms241210238
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