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Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia
The gold standard for diagnosing invasive candidiasis still relies on blood cultures, which are inefficient and time-consuming to analyze. We developed an in-house qPCR assay to identify the 5 major Candida species in 78 peripheral blood (PB) samples from ICU patients at risk of candidemia. Blood cu...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299639/ https://www.ncbi.nlm.nih.gov/pubmed/37367571 http://dx.doi.org/10.3390/jof9060635 |
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author | Felix, Gabriel N. de Freitas, Vera L. T. da Silva Junior, Afonso R. Magri, Marcello M. C. Rossi, Flavia Sejas, Odeli N. E. Abdala, Edson Malbouisson, Luiz M. S. Guimarães, Thais Benard, Gil Del Negro, Gilda M. B. |
author_facet | Felix, Gabriel N. de Freitas, Vera L. T. da Silva Junior, Afonso R. Magri, Marcello M. C. Rossi, Flavia Sejas, Odeli N. E. Abdala, Edson Malbouisson, Luiz M. S. Guimarães, Thais Benard, Gil Del Negro, Gilda M. B. |
author_sort | Felix, Gabriel N. |
collection | PubMed |
description | The gold standard for diagnosing invasive candidiasis still relies on blood cultures, which are inefficient and time-consuming to analyze. We developed an in-house qPCR assay to identify the 5 major Candida species in 78 peripheral blood (PB) samples from ICU patients at risk of candidemia. Blood cultures and (1,3)-β-D-glucan (BDG) testing were performed concurrently to evaluate the performance of the qPCR. The qPCR was positive for DNA samples from all 20 patients with proven candidemia (positive PB cultures), showing complete concordance with Candida species identification in blood cultures, except for detection of dual candidemia in 4 patients, which was missed by blood cultures. Additionally, the qPCR detected Candida species in six DNA samples from patients with positive central venous catheters blood (CB) but negative PB cultures. BDG values were similarly high in these six samples and the ones with proven candidemia, strongly suggesting the diagnosis of a true candidemia episode despite the negative PB cultures. Samples from patients neither infected nor colonized yielded negative results in both the qPCR and BDG testing. Our qPCR assay was at least as sensitive as blood cultures, but with a shorter turnaround time. Furthermore, negative results from the qPCR provided strong evidence for the absence of candidemia caused by the five major Candida species. |
format | Online Article Text |
id | pubmed-10299639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102996392023-06-28 Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia Felix, Gabriel N. de Freitas, Vera L. T. da Silva Junior, Afonso R. Magri, Marcello M. C. Rossi, Flavia Sejas, Odeli N. E. Abdala, Edson Malbouisson, Luiz M. S. Guimarães, Thais Benard, Gil Del Negro, Gilda M. B. J Fungi (Basel) Article The gold standard for diagnosing invasive candidiasis still relies on blood cultures, which are inefficient and time-consuming to analyze. We developed an in-house qPCR assay to identify the 5 major Candida species in 78 peripheral blood (PB) samples from ICU patients at risk of candidemia. Blood cultures and (1,3)-β-D-glucan (BDG) testing were performed concurrently to evaluate the performance of the qPCR. The qPCR was positive for DNA samples from all 20 patients with proven candidemia (positive PB cultures), showing complete concordance with Candida species identification in blood cultures, except for detection of dual candidemia in 4 patients, which was missed by blood cultures. Additionally, the qPCR detected Candida species in six DNA samples from patients with positive central venous catheters blood (CB) but negative PB cultures. BDG values were similarly high in these six samples and the ones with proven candidemia, strongly suggesting the diagnosis of a true candidemia episode despite the negative PB cultures. Samples from patients neither infected nor colonized yielded negative results in both the qPCR and BDG testing. Our qPCR assay was at least as sensitive as blood cultures, but with a shorter turnaround time. Furthermore, negative results from the qPCR provided strong evidence for the absence of candidemia caused by the five major Candida species. MDPI 2023-05-31 /pmc/articles/PMC10299639/ /pubmed/37367571 http://dx.doi.org/10.3390/jof9060635 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Felix, Gabriel N. de Freitas, Vera L. T. da Silva Junior, Afonso R. Magri, Marcello M. C. Rossi, Flavia Sejas, Odeli N. E. Abdala, Edson Malbouisson, Luiz M. S. Guimarães, Thais Benard, Gil Del Negro, Gilda M. B. Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia |
title | Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia |
title_full | Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia |
title_fullStr | Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia |
title_full_unstemmed | Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia |
title_short | Performance of a Real-Time PCR Assay for the Detection of Five Candida Species in Blood Samples from ICU Patients at Risk of Candidemia |
title_sort | performance of a real-time pcr assay for the detection of five candida species in blood samples from icu patients at risk of candidemia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299639/ https://www.ncbi.nlm.nih.gov/pubmed/37367571 http://dx.doi.org/10.3390/jof9060635 |
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